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http://dx.doi.org/10.12717/DR.2022.26.1.1

Signal Transduction of C-Terminal Phosphorylation Regions for Equine Luteinizing Hormone/Chorionic Gonadotropin Receptor (eLH/CGR)  

Byambaragchaa, Munkhzaya (Institute of Genetic Engineering, Hankyong National University)
Joo, Hyo-Eun (Division of Animal Science, School of Animal Life Convergence Sciences, Hankyong National University)
Kim, Sang-Gwon (Division of Animal Science, School of Animal Life Convergence Sciences, Hankyong National University)
Kim, Yean-Ji (Division of Animal Science, School of Animal Life Convergence Sciences, Hankyong National University)
Park, Gyeong-Eun (Division of Animal Science, School of Animal Life Convergence Sciences, Hankyong National University)
Min, Kwan-Sik (Institute of Genetic Engineering, Hankyong National University)
Publication Information
Development and Reproduction / v.26, no.1, 2022 , pp. 1-12 More about this Journal
Abstract
This study aimed to investigate the signal transduction of phosphorylation sites at the carboxyl (C)-terminal region of equine luteinizing hormone/chorionic gonadotropin receptor (eLH/CGR). The eLH/CGR has a large extracellular domain of glycoprotein hormone receptors within the G protein-coupled receptors. We constructed a mutant (eLH/CGR-t656) of eLH/CGR, in which the C-terminal cytoplasmic tail was truncated at the Phe656 residue, through polymerase chain reaction. The eLH/CGR-t656 removed 14 potential phosphorylation sites in the intracellular C-terminal region. The plasmids were transfected into Chinese hamster ovary (CHO)-K1 and PathHunter Parental cells expressing β-arrestin, and agonist-induced cAMP responsiveness was analyzed. In CHO-K1 cells, those expressing eLH/CGR-t656 were lower than those expressing eLH/CGR wild-type (eLH/CGR-wt). The EC50 of the eLH/CGR-t656 mutant was approximately 72.2% of the expression observed in eLH/CGR-wt. The maximal response in eLH/CGR-t656 also decreased to approximately 43% of that observed in eLH/CGR-wt. However, in PathHunter Parental cells, cAMP activity and maximal response of the eLH/CGR-t656 mutant were approximately 173.5% and 100.8%, respectively, of that of eLH/CGR-wt. These results provide evidence that the signal transduction of C-terminal phosphorylation in eLH/CGR plays a pivotal role in CHO-K1 cells. The cAMP level was recovered in PathHunter Parental cells expressing β-arrestin. We suggest that the signal transduction of the C-terminal region phosphorylation sites is remarkably different depending on the cells expressing β-arrestin in CHO-K1 cells.
Keywords
equine luteinizing hormone/chorionic gonadotropin receptor (eLH/CGR); Chinese hamster ovary (CHO)-K1 cells; PathHunter Parental cells; Phosphorylation sites; Signal transduction;
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Times Cited By KSCI : 10  (Citation Analysis)
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