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http://dx.doi.org/10.12717/DR.2021.25.4.313

Lin28 and Imp are Required for Stability of Bowl Transcripts in Hub Cells of the Drosophila Testis  

To, Van (School of Biological Sciences and Technology, Chonnam National University)
Kim, Hyun Ju (School of Biological Sciences and Technology, Chonnam National University)
Jang, Wijeong (School of Biological Sciences and Technology, Chonnam National University)
Sreejith, Perinthottathil (School of Biological Sciences and Technology, Chonnam National University)
Kim, Changsoo (School of Biological Sciences and Technology, Chonnam National University)
Publication Information
Development and Reproduction / v.25, no.4, 2021 , pp. 313-319 More about this Journal
Abstract
Hub cells comprise a niche for germline stem cells and cyst stem cells in the Drosophila testis. Hub cells arise from common somatic gonadal precursors in embryos, but the mechanism of their specification is still poorly understood. Here we find that RNA binding proteins Lin28 and Imp mediate transcript stability of Bowl, a known hub specification factor; Bowl transcripts were reduced in the testis of Lin28 and Imp mutants, and also when RNA-mediated interference against Lin28 or Imp was expressed in hub cells. In tissue culture Luciferase assays involving the Bowl 3'UTR, stability of Luc reporter transcripts depended on the Bowl 3'UTR and required Lin28 and Imp. Our findings suggest that proper Bowl function during hub cell specification requires Lin28 and Imp in the testis hub cells.
Keywords
Lin28; Imp; Niche; Stem cells; Testis; Bowl; Drosophila;
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