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http://dx.doi.org/10.12717/DR.2021.25.4.235

Analysis of Tissue-Specific Interferon Regulatory Factor 3 (IRF3) Gene Expression against Viral Infection in Paralichthys olivaceus  

Kim, Kyung-Hee (Fish Genetics and Breeding Research Center, National Institute of Fisheries Science)
Lee, Sanghyun (Fish Genetics and Breeding Research Center, National Institute of Fisheries Science)
Park, Jong-Won (Fish Genetics and Breeding Research Center, National Institute of Fisheries Science)
Jung, Hyo Sun (Fish Genetics and Breeding Research Center, National Institute of Fisheries Science)
Kim, Julan (Fish Genetics and Breeding Research Center, National Institute of Fisheries Science)
Yang, Hyerim (Fish Genetics and Breeding Research Center, National Institute of Fisheries Science)
Lee, Jeong-Ho (Fish Genetics and Breeding Research Center, National Institute of Fisheries Science)
Lee, Dain (Fish Genetics and Breeding Research Center, National Institute of Fisheries Science)
Publication Information
Development and Reproduction / v.25, no.4, 2021 , pp. 235-244 More about this Journal
Abstract
Interferon Regulatory Factor 3 (IRF3) is a member of interferon-regulated transcription factor family and is known to play an important role in the innate immune response against viral infections. In this study, the expression of IRF3 in different tissues, developmental stages, and stocking densities of olive flounder was investigated. The expression of IRF3 was observed to gradually increase in early-stage juvenile fish. The highest expression was observed in later-stage juvenile fish when immune tissues were formed. High IRF3 expression was observed in the muscles and the brain tissues. The expression of IRF3 was observed in fish at different stocking densities after viral hemorrhagic septicemia virus (VHSV) infection. It yielded an interesting expression pattern in the muscles and the brain tissues of fish stocked at low density. These observations can be used as basic data for the study of the expression of immune response-related genes against viruses based on stocking density and immune systems in other fish species.
Keywords
Olive flounder Paralichthys olivaceus; Gene expression; Interferon Regulatory Factor 3 (IRF3); Viral hemorrhagic septicemia virus (VHSV); Stocking densities;
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1 Noh GE, Kim WJ, Kim HC, Park CJ, Park JW (2017) Daily rhythms and effect of short-term starvation on the of health parameters in olive flounder Paralichthys olivaceus. Korean J Fish Aquatic Sci 50:534-540.   DOI
2 Oh DH, Song JW, Kim MG, Lee BJ, Kim KW, Han HS, Lee KJ (2013) Effect of food particle size, stocking density and feeding frequency on the growth performance of juvenile Korean rockfish Sebastes schlegelii. Korean J Fish Aquatic Sci 46:407-412.   DOI
3 Pestka S, Langer JA, Zoon KC, Samuel CE (1987) Interferons and their actions. Annu Rev Biochem 56:727-777.   DOI
4 Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:e45.   DOI
5 Rowland SJ, Mifsud C, Nixon M, Boyd P (2006) Effects of stocking density on the performance of the Australian freshwater silver perch (Bidyanus bidyanus) in cages. Aquaculture 253:301-308.   DOI
6 Rozen S, Skaletsky H. 2000. Primer3 on the WWW for general users and for biologist programmers. In: Misener S, Krawetz SA (eds), Bioinformatics Methods and Protocols: Methods in Molecular biology. Humana Press, Totowa, NJ, pp 365-386.
7 Tanaka Y, Chen ZJ (2012) STING specifies IRF3 phosphorylation by TBK1 in the cytosolic DNA signaling pathway. Sci Signal 5:ra20.   DOI
8 Isshiki T, Nishizawa T, Kobayashi T, Nagano T, Miyazaki T (2001) An outbreak of VHSV (viral hemorrhagic septicemia virus) infection in farmed Japanese flounder Paralichthys olivaceus in Japan. Dis Aquat Organ 47:87-99.   DOI
9 Kim KK, Hwang HK, Kim HC, Kim KW, Cho JW, Jung MH (2016) Olive Flounder Culture Standard Manual. National Institute of Fisheries Science, Busan, Korea.
10 Tamura T, Yanai H, Savitsky D, Taniguchi T (2008) The IRF family transcription factors in immunity and oncogenesis. Annu Rev Immunol 26:535-584.   DOI
11 Xu X, Lai Q, Gu M, Liu D, Hou Q, Liu X, Mi Y, Sun Z, Wang H, Lin G, Hu C (2015) Fish IRF3 up-regulates the transcriptional level of IRF1, IRF2, IRF3 and IRF7 in CIK cells. Fish Shellfish Immunol 47:978-985.   DOI
12 Holm JC, Refstie T, Bo S (1990) The effect of fish density and feeding regimes on individual growth rate and mortality in rainbow trout (Oncorhynchus mykiss). Aquaculture 89:225-232.   DOI
13 Huang B, Qi ZT, Xu Z, Nie P (2010) Global characterization of interferon regulatory factor (IRF) genes in vertebrates: Glimpse of the diversification in evolution. BMC Immunol 11:22.   DOI
14 Kim KS (2009) Study on early development and metamorphosis of Paralichthys olivaceus. Ph.D. Dissertation, Chonnam National University, Gwangju, Korea.
15 Kim WS, Kim SR, Kim D, Kim JO, Park MA, Kitamura SI, Kim HY, Kim DH, Han HJ, Jung SJ, Oh MJ (2009) An outbreak of VHSV (viral hemorrhagic septicemia virus) infection in farmed olive flounder Paralichthys olivaceus in Korea. Aquaculture 296:165-168.   DOI
16 Lengyel P (1982) Biochemistry of interferons and their actions. Annu Rev Biochem 51:251-282.   DOI
17 Bjornsson B (1994) Effects of stocking density on growth rate of halibut (Hippoglossus hippoglossus L.) reared in large circular tanks for three years. Aquaculture 123:259-270.   DOI
18 Krishnan R, Kurcheti PP, Mushtaq Z, Jeena K, Naik VT (2019) Interferon-regulatory factors, IRF3 and IRF7 in Asian seabass, Lates calcarifer: Characterization, ontogeny and transcriptional modulation upon challenge with nervous necrosis virus. Fish Shellfish Immunol 89:468-476.   DOI
19 Magnadottir B (2006) Innate immunity of fish (overview). Fish Shellfish Immunol 20:137-151.   DOI
20 Barnes B, Lubyova B, Pitha PM (2002) On the role of IRF in host defense. J Interferon Cytokine Res 22:59-71.   DOI
21 Espinosa V, Dutta O, McElrath C, Du P, Chang YJ, Cicciarelli B, Pitler A, Whitehead I, Obar JJ, Durbin JE, Kotenko SV, Rivera A (2017) Type III interferon is a critical regulator of innate antifungal immunity. Sci Immunol 2:5357.
22 Holland JW, Bird S, Williamson B, Woudstra C, Mustafa A, Wang T, Zou J, Blaney SC, Collet B, Secombes CJ (2008) Molecular characterization of IRF3 and IRF7 in rainbow trout, Oncorhynchus mykiss: Functional analysis and transcriptional modulation. Mol Immunol 46:269-285.   DOI
23 Kim KH, Cho DH, Joo MS, Choi KM, Park CI, Kim MC (2020) Molecular characterization and expression analysis of the interferon regulatory factor 3 (IRF3) gene from red sea bream Pagrus major. J Fish Mar Sci Educ 32:1153-1167.   DOI
24 Au WC, Moore PA, Lowther W, Juang YT, Pitha PM (1995) Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes. Proc Natl Acad Sci USA 92:11657-11661.   DOI
25 Eh YY (2011) Productivity of the flounder stocking density on the flounder culture farms. J Fish Bus Adm 42:85-96.
26 Wang Z, Ji J, Peng D, Ma F, Cheng G, Qin FXF (2016) Complex regulation pattern of IRF3 activation revealed by a novel dimerization reporter system. J Immunol 196:4322-4330.   DOI
27 Okorie OE, Bae JY, Kim KW, Son MH, Kim JW, Bai SC (2013) Optimum feeding rates in juvenile olive flounder, Paralichthys olivaceus, at the optimum rearing temperature. Aquac Nutr 19:267-277.   DOI
28 Refstie T (1977) Effect of density on growth and survival of rainbow trout. Aquaculture 11:329-334.   DOI
29 Seo J (2020) Effects of stocking density on productivity and stress of olive flounder (Paralichthys olivaceus) in recirculation aquaculture system. M.S. Thesis, Pukyong National University, Busan, Korea.
30 Yeow WS, Au WC, Juang YT, Fields CD, Dent CL, Gewert DR, Pitha PM (2000) Reconstitution of virus-mediated expression of interferon α genes in human fibroblast cells by ectopic interferon regulatory factor-7. J Biol Chem 275:6313-6320.   DOI
31 Collins SE, Noyce RS, Mossman KL (2004) Innate cellular response to virus particle entry requires IRF3 but not virus replication. J Virol 78:1706-1717.   DOI
32 Brock JA (1992) Current diagnostic methods for agents and diseases of farmed marine shrimp. In: Proceeding Asian Interchange Program Workshop on the Diseases of Cultured Penaeid Shrimp, Honolulu, HI, pp 209-231.
33 Kong HJ, Hong GE, Kim WJ, Kim YO, Nam BH, Lee CH, Do JW, Lee JH, Lee SJ, Kim KK (2009) Cloning and characterization of hypusine-containing protein eIF5A from the olive flounder Paralichthys olivaceus. Comp Biochem Physiol B Biochem Mol Biol 153:281-287.   DOI
34 Zapata A, Diez B, Cejalvo T, Gutierrez-de Frias C, Cortes A (2006) Ontogeny of the immune system of fish. Fish Shellfish Immunol 20:126-136.   DOI
35 Main KL, Fulks W (1992) Diseases of cultured penaeid shrimp in Asia and the United States. The Oceanic Institute, Honolulu, HI.
36 Han KH, Kim KS, Lee SH (2007) Morphological studies on the digestive tracts of the larvae and juveniles of the flounder, Paralichthys olivaceus. Dev Reprod 11:121-125.
37 Hiscott J, Pitha P, Genin P, Nguyen H, Heylbroeck C, Mamane Y, Algarte M, Lin R (1999) Triggering the interferon response: The role of IRF-3 transcription factor. J Interferon Cytokine Res 19:1-13.   DOI