Browse > Article
http://dx.doi.org/10.12717/DR.2020.24.4.277

Susceptibility of Koi, Koi×Red Common Carp, and Red Common Carp×Koi to Koi Herpesvirus (KHV)  

Hwang, Ju-Ae (Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS))
Kim, Jung Eun (Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS))
Kim, Hyeong Su (Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS))
Park, Junseong (Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS))
Lee, Jeong-Ho (Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS))
Publication Information
Development and Reproduction / v.24, no.4, 2020 , pp. 277-285 More about this Journal
Abstract
The disease-causing koi herpes virus (KHV), also known as cyprinid herpesvirus-3 (CyHV3), causes mass mortality of koi and carp. Koi (Cyprinus carpio) is a host for KHV, one of 12 virus species in the Alloherpesviridae family. We examined the effects of KHV disease koi (KK), and on koi×red common carp (KR) and red common carp×koi (RK) cross, using a virus challenge test. The infected fish had clinical signs that included gill necrosis and skin lesions. The RK and KR were highly more resistant (cumulative mortality: RK; 6% and KR; 8%) to KHV infection than KK fish (cumulative mortality: 28%). KHV DNA was confirmed in the tissues of all dead fish in groups by use of polymerase chain reaction (PCR), and the presence of the KHV protein in kidney was confirmed by immunohistochemistry. Histological analysis showed severe gill lesions and fusion of the lamellae in KK fish, but less severe damage in RK fish. In immunohistochemistry analysis, the KHV protein localized in the cytoplasm of infected kidney cells of KK, but the cross groups had lower levels of KHV antigen. Our data indicate that the cross groups had increased resistance to KHV disease.
Keywords
Koi; Breeding; Koi herpes virus (KHV); Gill; Kidney;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Cheng L, Chen CY, Tsai MA, Wang PC, Hsu JP, Chern R, Chen SC (2011) Koi herpesvirus epizootic in cultured carp and koi, Cyprinus carpio L., in Taiwan. J Fish Dis 34:547-554.   DOI
2 Haenen OLM, Way K, Bergmann SM, Ariel E (2004). The emergence of koi herpesvirus and its significance to European aquaculture. Bull Eur Assoc Fish Pathol 24:293-307.
3 Zhao XJ, Liu ZZ, Li XS, Wang Y, Wang CH (2012) Major histocompatibility complex class II B allele polymorphism and its association with resistance/susceptibility to Aeromonas hydrophila in the "whole white" color patterns of Cyprinus carpio var. color. J Fish Chin 19:399-407.
4 Bercovier H, Fishman Y, Nahary R, Sinai S, Zlotkin A, Eyngor M, Gilad O, Eldar A, Hedrick RP (2005) Cloning of the koi herpesvirus (KHV) gene encoding thymidine kinase and its use for highly sensitive PCR based diagnosis. BMC Microbiol 5:13-22.   DOI
5 Pikarsky E, Ronen A, Abramowitz J, Levavi-Sivan B, Hutoran M, Shapira Y, Steinitz M, Perelberg A, SofferD, Kotler M (2004) Pathogenesis of acute viral disease in fish induced by the carp interstitial nephritis and gill necrosis virus. J Virol 78:9544-9551.   DOI
6 Aoki T, Hirono I, Kurokawa K, Fukuda, Nahary R, Eldar A, Davison AJ, Waltzek TB, Bercovier B, Hedrick RP (2007) Genome sequences of three koi herpesvirus isolates representing the expending distribution of an emerging disease threatening koi and common carp worldwide. J Virol 81:5058-5065.   DOI
7 Aoki T, Takanoa T, Unajaka S, Takagi M, Kim YR, Park SB, Kondoa H, Hironoa I, Saito-Taki T, Hikimac JI, Jung TS (2011) Generation of monoclonal antibodies specific for ORF68 of koi herpesvirus. Comp Immunol Microbiol Infect Dis 34:209-216.   DOI
8 Liu J, Liu ZZ, Zhao XJ, Wang CH (2014) MHC class IIα alleles associated with resistence to Aeromonas hydrophila in purse red common carp, Cyprinus carpio Linnaeus. J Fish Dis 37:571-575.   DOI
9 Hwang JA, Kim JE, Kim HS, Lee JH (2017) Immune response to koi herpesvirus (KHV) of koi and koi×red common carp (Cyprinus carpio). Dev Reprod 21:361-370.   DOI
10 ICTV (2020) International Committee on Taxonomy of Viruses ICTV. Available from: http://www.ictvonline.org. Accessed at September 28, 2020.
11 Li XS, Liu ZZ, Zhao XJ, Ma XQ, Feng W, Wang CH (2011) Major histocompatibility complex DAB allele polymorphism and its association with resistance/susceptibility to Aeromonas hydrophila in "whole red" color patterns of Cyprinus carpio var. color. J Fish Chin 35:1293-1301.
12 Bretzinger A, Fischer-Scherl T, Oumouna M, Hoffmann R, Truyen U (1999) Mass mortalities in koi carp, Cyprinus carpio, associated with gill and skin disease. Bull Eur Assoc Fish Pathol 19:182-199.
13 Bergmann SM, Riechart M, Fichtner D, Lee P, Kempter J (2010a) Investigation on the Disgnostic sensitivity of molecular tools used for detection of koi herpes virus. J Virol Methods 163:229-233.   DOI
14 Bergmann SM, Sadowski J, Kielpinski M, Bartlomiejczyk M, Fichtner D, Riebe R, Lenk M, Kempter J (2010b) Susceptibility of koi×crucian carp and koi×goldfish hybrids to koi herpesvirus (KHV) and development of KHV disease (KHVD). J Fish Dis 33:267-272.   DOI
15 Bergmann SM, Schutze H, Fischer U, Fichtner D, Riechardt M, Meyer K, Schrudde, Kempter J (2009) Detection of koi herpes virus (KHV) genome in apparently healthy fish. Bull Eur Assoc Fish Pathol 29:145-152.
16 Chevassus B, Dorson M (1990) Genetics of resistance to disease in fishes. Aquac 85:83-107.   DOI
17 Dishon A, Perelberg A, Bishara-Shieban J, Ilouze M, Davidovich, M, Werker, S, Kotler M. (2005) Detection of carp interstitial nephritis and gill necrosis virus in fish droppings. Appl Environ Microbiol 71:7285-7291.   DOI
18 Gilad O, Yun S, Andree KB, Adkison MA, Zlotkin A, Bercovier H, Eldar A, Hedrick RP (2002) Initial characteristics of koi herpesvirus and development of a polymerase chain reaction assay to detect the virus in koi, Cyprinus carpio koi. Dis Aquat Org 48:101-108.   DOI
19 Hedrick RP, Gilad O, Yun S, Spangenberg JV, Marty, GD, Nordhausen RW, Kebus MJ, Bercovier H, Eldar A (2000) A herpesvirus associated with mass mortality of juvenile and adult koi, a strain of common carp. J Aquat Anim Health 12:44-57.   DOI
20 Rahmati-Holasoo H, Zargar A, Ahmadivand S, Shokrpoor S, Ezhari S, Ebrahimzadeh Mousavi HA (2016) First detection of koi herpesvirus from koi, Cyprinus carpio L. experiencing mass mortalities in Iran: clinical, histopathological and molecular study. J Fish Dis 39:1153-1163.   DOI
21 Roizman B, Pellet P (2001) The family herpesviridae: a brief introduction. In: Knipe DM, Howley PM (eds), Fields Virology. 4th ed. Lippincott Williams & Wilkins, Philadelphia, PA, pp 2381-2397.
22 Shapira Y, Magen Y, Zak T, Ktler M, Hulata G, Levavi-Sivan B (2005) Differential resistance to koi herpes virus (KHV)/carp interstitial nephritis and gill necrosis virus (CNGV) among common carp (Cyprinus carpio L.) strains and crossbreds. Aquac 245:1-11.   DOI
23 Gilad O, Yun S, Zagmutt-Vergara FJ, Leutenegger CM, Bercovier HV, Hedrick RP (2004) Concentrations of a koi herpesvirus (KHV) tissues of experimentally-infected Cyprinus carpio koi as assessed by real-time TaqMan PCR. Dis Aquat Org 60:179-187.   DOI
24 OIE (2007) Koi herpesvirus disease. In: Manual of Diagnostic Tests for Aquatic Animals. World Organisation for Animal Health, Paris, France.
25 Sano M, Ito T, Kurita J, Yanai T, Watanabe N, Miwa S, Lida T (2004) First detection of koi herpesvirus in cultured common carp Cyprinus carpio in Japan. Fish Pathol 39:165-167.   DOI
26 Cho MY, Won KM, Kim JW, Jee BY, Park MA, Hong S (2014) Detection of koi herpesvirus (KHV) in healthy cyprinid stock. Dis Aquat Org 112:29-36.   DOI
27 Costes B, Raj VS, Michel B, Fournier G, Thirion M, Gillet L, Mast J, Lieffrig F, Bremont M, Vanderplasschen A (2009) The major portal of entry of Koi herpesvirus in Cyprinus carpio is the Skin. J Virol 83:2819-2830.   DOI
28 Dixon PF, Joiner CL, Way K, Reese RA, Jeney G, Jeney Z (2009) Comparison of the resistance of selected families of a common carp, Cyprinus carpio L., to koi herpesvirus: preliminary study. J Fish Dis 32:1035-1039.   DOI
29 Haramoto E, Kitajima M, Katayama H, Ito T, Ohgaki S (2009) Development of virus concentration methods for detection of koi herpesvirus in water. J Fish Dis 32:297-300.   DOI
30 Gomez DK, Joh SJ, Jang H, Shin SP, Choresca CH Jr, Han JE, Kim JH, Jun JW, Park SC (2011) Detection of koi herpesvirus (KHV) from koi (Cyprinus carpio koi) broodstock in South Korea. Aquac 311:42-47.   DOI
31 Hedrick RP, Gilad O, Yun SC, McDowell TS, Waltzek TB, Kelley GO, Adkison MA (2005) Initial isolation and characterization of a herpes-like virus (KHV) from koi and common carp. Bull Fish Res Agency S2: 1-7.
32 Hedrick RP, Waltzek TB, McDowell TS (2006) Susceptibility of koi carp, common carp, Goldfish, and goldfish×common carp hybrids to cyprinid herpesvirus-2 and herpesvirus-3. J Aquat Anim Health 18:26-34.   DOI
33 Dong C, Weng S, Li W, Li X, Yi Y, Liang Q, He J (2011) Characterization of a new cell line from caudal fin of koi, Cyprinus carpio koi, and first isolation of cyprinid herpesvirus 3 in China. Virus Res 161:140-149.   DOI
34 Wang CH, Li SF, Xiang SP, Wang J, Liu ZG, Pang ZY, Duan JP, Xu ZB (2006) Additive, dominance genetic effects for growth-related traits in common carp, Cyprinus carpio L. Aquac Res 37:1481-1486.   DOI
35 Minamoto T, Honjo MN, Yamanaka H, Uchii K, Kawabata Z (2012) Nationwide Cyprinid herpesvirus 3 contamination in natural rivers of Japan. Res Vet Sci 93:508-514.   DOI
36 OIE (2013) Manual of Diagnostic Tests for Aquatic Animals. Office International Des Epizooties, Paris, France.
37 Rakus KL, Wiegertjes GF, Jurecka PM, Walker PD, Pilarczyk A, Irnazarow I (2009) Major histocompatibility (MH) class II B gene polymorphism influences disease resistance of common carp (Cyprinus carpio L.). Aquaculture 288:44-50.   DOI
38 Sano M, Ito T, Kurita J, Miwa S, Lida T (2005) Diagnosis of koi Herpesvirus (KHV) disease in Japan. Bull Fish Res Agency S2 :59-64.
39 Tu C, Weng MC, Shiau JR, Lin SY (2004) Detection of koi herpesvirus in koi Cyprinus carpio in Taiwan. Fish Pathol 39:109-110.   DOI