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http://dx.doi.org/10.12717/DR.2020.24.3.225

Mature and Bi-Sexual Phase Gonad Occurrence in Cultured Red Spotted Grouper, Epinephelus akaara  

Hwang, In Joon (Inland Fisheries Research Institute, National Institute of Fisheries Science)
Min, Byung Hwa (Research and Development Planning Division, National Institute of Fisheries Science)
Baek, Hea Ja (Dept. of Marine Biology, Pukyong National University)
Publication Information
Development and Reproduction / v.24, no.3, 2020 , pp. 225-230 More about this Journal
Abstract
This study reports the presence of mature and bi-sexual phase gonads in red spotted grouper, Epinephelus akaara after less than a year of cultivation in a commercial indoor tank and a net cage. In December 2018, juveniles were placed in an indoor tank and cultured for five months. In June 2019, the fish were transferred to a net cage and cultured until September. The rearing temperatures ranged from 19.86℃-24.65℃ in the indoor tank and 21.86℃-27.65℃ in the net cage. During the net cage culture period, specimens were randomly selected for histological gonad examination. The highest gonadosomatic index (GSI) value was measured in July (3.38±2.53), and dramatically decreased in August (0.44±0.21) and September (0.42±0.30). In July, some mature fish showed signs of vitellogenic stage oocyte development (vitellogenic and oil droplet stage oocytes), but immature fish were in an early developmental stage containing peri-nucleolus stage (PNS) oocytes. Bi-sexual phase gonads containing spermatocytes and spermatids were observed in the lumen and several PNS oocytes. By August and September, most specimens showed early-stage ovary development. However, mature testis (in August) and bi-sexual phase gonads (in September) were also observed. These results provide evidence for early puberty and hermaphroditism in the red spotted grouper.
Keywords
Bi-sexual phase gonad; Oocyte; Red spotted grouper; Spermatocyte; Spermatid;
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