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http://dx.doi.org/10.12717/DR.2019.23.3.285

A Maternal Transcription Factor, Junction Mediating and Regulatory Protein is Required for Preimplantation Development in the Mouse  

Lin, Zi-Li (College of Animal Sciences, Jilin University)
Li, Ying-Hua (Department of Animal Sciences, Yanbian University)
Jin, Yong- Xun (College of Animal Sciences, Jilin University)
Kim, Nam-Hyung (College of Animal Sciences, Jilin University)
Publication Information
Development and Reproduction / v.23, no.3, 2019 , pp. 285-295 More about this Journal
Abstract
Junction-mediating and regulatory protein (JMY) is a regulator of both transcription and actin filament assembly. The actin-regulatory activity of JMY is based on a cluster of three actin-binding Wiskott-Aldrich syndrome protein homology 2 (WH2) domains that nucleate actin filaments directly and promote nucleation of the Arp2/3 complex. In addition to these activities, we examined the activity of JMY generation in early embryo of mice carrying mutations in the JMY gene by CRISPR/Cas9 mediated genome engineering. We demonstrated that JMY protein shuttled expression between the cytoplasm and the nucleus. Knockout of exon 2, CA (central domain and Arp2/3-binding acidic domain) and NLS-2 (nuclear localization signal domain) on the JMY gene by CRISPR/Cas9 system was effective and markedly impeded embryonic development. Additionally, it impaired transcription and zygotic genome activation (ZGA)-related genes. These results suggest that JMY acts as a transcription factor, which is essential for the early embryonic development in mice.
Keywords
Transcription; Mouse; Embryo; Blastocyst;
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