Browse > Article
http://dx.doi.org/10.12717/DR.2019.23.3.231

Interleukin-8 (IL-8) Expression in the Olive Flounder (Paralichthys olivaceus) against Viral Hemorrhagic Septicemia Virus (VHSV) Challenge  

Kim, Kyung-Hee (Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS))
Kim, Hyun Chul (Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS))
Park, Choul-Ji (Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS))
Park, Jong-Won (Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS))
Lee, Young Mee (Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS))
Kim, Woo-Jin (Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS))
Publication Information
Development and Reproduction / v.23, no.3, 2019 , pp. 231-238 More about this Journal
Abstract
Interleukin-8 (IL-8) is an inflammatory cytokine that plays an important role in the inflammatory response through the activation of neutrophil cells. The expression of IL-8 was investigated in early developmental stages of the olive flounder and in tissues of 8-month-old individuals. The expression of IL-8 increased after the initiation of the immune system rather than at the early stage of development, and high expression was observed in the gills and spleen, the organs associated with immunity and metabolism. In addition, IL-8 expression after infection by viral hemorrhagic septicemia virus significantly increased in the fin, gill, muscles, and spleen. These results suggest that IL-8 is closely related to inflammation and immune regulation in the immune response of the olive flounder and may be used as a basis for studies on the immune systems of other fish.
Keywords
Olive flounder Paralichthys olivaceus; Gene expression; Interleukin-8 (IL-8); Viral hemorrhagic septicemia virus (VHSV);
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Zhao B, Katagiri T, Kondo H, Hirono I (2015) Comparative analysis of two types of CXCL8 from Japanese flounder (Paralichthys olivaceus). Dev Comp Immunol 52:37-47.   DOI
2 Zhou S, Mu Y, Ao J, Chen X (2018) Molecular characterization and functional activity of CXCL8_L3 in large yellow croaker Larimichthys crocea. Fish Shellfish Immunol 75:124-131.   DOI
3 Zlotnik A, Yoshie O (2012) The chemokine superfamily revisited. Immunity 36:705-716.   DOI
4 Corripio-Miyar Y, Bird S, Tsamopoulos K, Secombes CJ (2007) Cloning and expression analysis of two proinflammatory cytokines, IL-$1{\beta}$ and IL-8, in haddock (Melanogrammus aeglefinus). Mol Immunol 44:1361-1373.   DOI
5 Beck G, Habicht GS (1996) Immunity and the inverte brates. Sci Am 275:60-66.   DOI
6 Chen L, He C, Baoprasertkul P, Xu P, Li P, Serapion J, Waldbieser G, Wolters W, Liu Z (2005) Analysis of a catfish gene resembling interleukin-8: cDNA cloning, gene structure, and expression after infection with Edwardsiella ictaluri. Dev Comp Immunol 29:135-142.   DOI
7 Chu CQ, Lu XJ, Li CH, Chen J (2014) Molecular characterization of a CXCL8-like protein from ayu and its effect on chemotaxis of neutrophils and monocytes/macrophages. Gene 548:48-55.   DOI
8 Fernandez EJ, Lolis E (2002) Structure, function, and inhibition of chemokines. Annu Rev Pharmacol Toxicol 42:469-499.   DOI
9 Duesund H, Nylund S, Watanabe K, Ottem KF, Nylund A (2010) Characterization of a VHS virus genotype III isolated from rainbow trout (Oncorhychus mykiss) at a marine site on the west coast of Norway. Virol J 7:19.   DOI
10 Esche C, Stellato C, Beck LA (2005) Chemokines: Key players in innate and adaptive immunity. J Invest Dermatol 125:615-628.   DOI
11 Fischer U, Koppang EO, Nakanishi T (2013) Teleost T and NK cell immunity. Fish Shellfish Immunol 35:197-206.   DOI
12 Guo M, Tang X, Sheng X, Xing J, Zhan W (2018) The effects of IL-$1{\beta}$, IL-8, G-CSF and TNF-$\alpha$ as molecular adjuvant on the immune response to an E. tarda subunit vaccine in flounder (Paralichthys olivaceus). Fish Shellfish Immunol 77:374-384.   DOI
13 Harun NO, Zou J, Zhang YA, Nie P, Secombes CJ (2008) The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8. Dev Comp Immunol 32:673-681.   DOI
14 Huising MO, Stolte E, Flik G, Savelkoul HF, Verburg-van Kemenade BM (2003) CXC chemokines and leukocyte chemotaxis in common carp (Cyprinus carpio L.) Dev Comp Immunol 27:875-888.   DOI
15 Kapp A, Zeck-Kapp G, Czech W, Schopf E (1994) The chemokine RANTES is more than a chemoattractant: Characterization of its effect on human eosinophil oxidative metabolism and morphology in comparison with IL-5 and GM-CSF. J Invest Dermatol 102:906-914.   DOI
16 Kim SM, Lee JI, Hong MJ, Park HS, Park SI (2003). Genetic relationship of the VHSV (viral hemorrhagic septicemia virus) isolated from cultured olive flounder, Paralichthys olivaceus in Korea. J Fish Pathol 16:1-12.
17 Laing KJ, Secombes CJ (2004) Chemokines. Dev Comp Immunol 28:443-460.   DOI
18 Kim WS, Kim SR, Kim DW, Kim JO, Park MA, Kitamura SI, Kim HY, Kim DH, Han HJ, Jung SJ, Oh MJ (2009) An outbreak of VHSV (viral hemorrhagic septicemia virus) infection in farmed olive flounder Paralichthys olivaceus in Korea. Aquaculture 296:165-168.   DOI
19 Kong HJ, Hong GE, Kim WJ, Kim YO, Nam BH, Lee CH, Do JW, Lee JH, Lee SJ, Kim KK (2009) Cloning and characterization of hypusine-containing protein eIF5A from the olive flounder Paralichthys olivaceus. Comp Biochem Physiol B Biochem Mol Biol 153:281-287.   DOI
20 Korean Statistical Information Service [KOSIS] (2018) 2018 survey on the status of Fish culture. Available at: http://kosis.kr. Accessed 23 July 2019.
21 Laing KJ, Zou JJ, Wang T, Bols N, Hirono I, Aoki T, Secombes CJ (2002) Identification and analysis of an interleukin 8-like molecule in rainbow trout Oncorhynchus mykiss. Dev Comp Immunol 26:433-444.   DOI
22 Lee EY, Park HH, Kim YT, Choi TJ (2001) Cloning and sequence analysis of the interleukin-8 gene from flounder (Paralichthys olivaceous). Gene 274:237-243.   DOI
23 Mu Y, Wang K, Ao J, Chen X (2015) Molecular characterization and biological effects of a CXCL8 homologue in large yellow croaker (Larimichthys crocea). Fish Shellfish Immunol 44:462-470.   DOI
24 Sun JS, Zhao L, Sun L (2011) Interleukin-8 of Cynoglossus semilaevis is a chemoattractant with immunoregulatory property. Fish Shellfish Immunol. 30:1362-1367.   DOI
25 Nguyen TTT, Nguyen HT, Wang PC, Chen SC (2017) Identification and expression analysis of two proinflammatory cytokines, TNF- $\alpha$ and IL-8, in cobia (Rachycentron canadum L.) in response to Streptococcus dysgalactiae infection. Fish Shellfish Immunol 67:159-171.   DOI
26 Noh GE, Kim WJ, Kim HC, Park CJ, Park JW (2017) Daily rhythms and effect of short-term starvation on the of health parameters in olive flounder Paralichthys olivaceus. Korean J Fish Aquat Sci 50:534-540.   DOI
27 OIE [World Organisation for Animal Health] (2018) Viral haemorrhagic septicaemia. In: Manual of Diagnostic Tests for Aquatic Animals. OIE, Paris, France.
28 Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:e45.   DOI
29 Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Misener, S, Krawetz SA (ed), Bioinformatics Methods and Protocols: Methods in Molecular Biology. Humana Press, Totowa, NJ, pp 365-386.
30 Taub DD, Anver M, Oppenheim JJ, Longo DL, MurphyWJ (1996). T lymphocyte recruitment by interleukin-8 (IL-8). IL-8-induced degranulation of neutrophils releases potent chemoattractants for human T lymphocytes both in vitro and in vivo. J Clin Invest 97:1931-1941.   DOI
31 Xiao Y, Yu L, Gui G, Gong Y, Wen X, Xia W, Yang H, Zhang L (2019) Molecular cloning and expression analysis of interleukin-8 and -10 in yellow catfish and in response to bacterial pathogen infection. BioMed Res Int 2019.
32 Tian C, Chen Y, Ao J, Chen X (2010) Molecular characterization and bioactivity of a CXCL13 chemokine in large yellow croaker Pseudosciaena crocea. Fish Shellfish Immunol 28:445-452.   DOI
33 Wang T, Liang J, Xiang X, Yuan J, Chen X, Xiang X (2019a) Functional identification and expressional responses of large yellow croaker (Larimichthys crocea) interleukin-8 and its receptor. Fish Shellfish Immunol 87:470-477.   DOI
34 Wang X, Ma G, Zhang R, Liu L, Zhu J, Zhu H (2019b) Molecular characterization and biological functioning of interleukin-8 in Siberian sturgeon (Acipenser baeri). Fish shellfish Immunol 90:91-101.   DOI