Browse > Article
http://dx.doi.org/10.12717/DR.2018.22.3.283

Genetic Distances of Paralichthys olivaceus Populations Investigated by PCR  

Yoon, Jong-Man (Dept. of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
Publication Information
Development and Reproduction / v.22, no.3, 2018 , pp. 283-288 More about this Journal
Abstract
The author carried out PCR-based genetic platform to investigate the hierarchical polar dendrogram of Euclidean genetic distances of one bastard halibut population, particularly for Paralichthys olivaceus, which was further connected with those of the other fish population, by involving with the precisely designed oligonucleotide primer sets. Eight oligonucleotides primers were used generating excessively alterating fragments, ranging in size of DNA bands from larger than approximately 100 bp to less than 2,000 bp. As regards average bandsharing value (BS) results, individuals from Hampyeong population (0.810) displayed lower bandsharing values than did individuals from Wando population (0.877). The genetic distance between individuals approved the existence of close relationship in the cluster II. Relatively, individuals of one bastard halibut population were fairly related to that of the other fish population, as shown in the polar hierarchical dendrogram of Euclidean genetic distances. The points of a noteworthy genetic distance between two P. olivaceus populations demonstrated this PCR procedure is one of the quite a few means for individuals and/or populations biological DNA investigates, for species security and proliferation of bastard halibut individuals in coastal region of the Korea.
Keywords
Euclidean genetic distances; Polar dendrogram; Bastard halibut population;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Kim SR, Ko SM, Choi H, Park JJ (2017) The first report of marine leech, Austrobdella sp. parasited on the wild flounder, Paralithytis olivaceus and histopathological characteristics of the host. J Fish Mar Sci Edu 29:1394-1404.
2 Kim JY, Sung GH, Lim JJ, Suo SA, Cho YR, Kim JH (2018) Effects of exposure to hexavalent chromium on hematological parameters and plasma components in flatfish, Paralichthys olivaceus. Korean J Environ Biol 36:124-130.   DOI
3 Lee HY, Yoo HK (2016) Effects of various diets on growth and body composition of juvenile olive flounder, Paralichthys olivaceus. Korean J Ichthyol 28:200-206.
4 Mamuris Z, Stamatis C, Bani M, Triantaphyllidis C (1999) Taxonomic relationships between four species of the Mullidae family revealed by three genetic methods: Allozymes, random amplified polymorphic DNA and mitochondrial DNA. J Fish Biol 55:572-587.   DOI
5 McCormack GP, Powell R, Keegan BF (2000) Comparative analysis of two populations of the brittle star Amphiura filiformis (Echinodermata: Ophiuroidae) with different life history strategies using RAPD markers. Mar Biotechnol 2:100-106.
6 Muchmore ME, Moy GW, Swanson WJ, Vacquier VD (1998) Direct sequencing of genomic DNA for characterization of a satellite DNA in five species of Eastern Pacific abalone. Mol Mar Biol Biotechnol 7:1-6.
7 Oh H, Yoon JM (2014) Genetic distances of three mollusk species investigated by PCR analysis. Dev Reprod 18:43-49.   DOI
8 Partis L, Wells RJ (1996) Identification of fish species using random amplified polymorphic DNA (RAPD). Mol Cell Probes 10:435-441.   DOI
9 Song YJ, Yoon JM (2013) Genetic differences of three Pollicipes mitella population identified by PCR analysis. Dev Reprod 17:199-205.   DOI
10 Tassanakajon A, Pongsomboon S, Jarayabhand P, Klinbunga S, Boonsaeng V (1998) Genetic structure in wild populations of black tiger shrimp (Penaeus monodon) using randomly amplified polymorphic DNA analysis. J Mar Biotechnol 6:249-254.
11 Yoon JM, Park HY (2002) Genetic similarity and variation in the cultured and wild crucian carp (Carassius carassius) estimated with random amplified polymorphic DNA. Asian-Australas J Anim Sci 15:470-476.   DOI
12 Chenyambuga SW, Hanotte O, Hirbo J, Watts PC, Kemp SJ, Kifaro GC, Gwakisa PS, Petersen PH, Rege JEO (2004) Genetic characterization of indigenous goats of sub-Saharan Africa using microsatellite DNA markers. Asian-Australas J Anim Sci 17:445-452.   DOI
13 Yoon JM, Kim JY (2004) Genetic differences within and between populations of Korean catfish (S. asotus) and bullhead (P. fulvidraco) analysed by RAPD-PCR. Asian-Australas J Anim Sci 17:1053-1061.   DOI
14 Yoke-Kqueen C, Radu S (2006) Random amplified polymorphic DNA analysis of genetically modified organisms. J Biotechnol 127:161-166.   DOI
15 Zhou L, Wang Y, Gui JF (2000) Analysis of genetic heterogeneity among five gynogenetic clones of silver crucian carp, Carassius auratus gibelio Block, based on detection of RAPD molecular markers. Cytogenet Cell Genet 88:133-139.   DOI
16 Bae SH, Kim KW, Kim SK, Kim JH, Kim JH (2017) Lethal toxicity and hematological changes exposed to nitrate in flatfish, Paralichthys olivaceus in biofloc and seawater. Environ Biol Res 35:373-379.   DOI
17 Callejas C, Ochando MD (1998) Identification of Spanish barbel species using the RAPD technique. J Fish Biol 53:208-215.   DOI
18 Diaz-Jaimes P, Uribe-Alcocer M (2003) Allozyme and RAPD variation in the eastern Pacific yellowfin tuna (Thunnus albacares). Fish Bull 101:769-777.
19 Gwakisa PS, Kemp SJ, Teale AJ (1994) Characterization of zebu cattle breeds in Tanzania using random amplified polymorphic DNA markers. Anim Genet 25:89-94.   DOI
20 Islam MS, Ahmed ASI, Azam MS, Alam MS (2005) Genetic analysis of three river populations of Catla catla (HAMILTON) using randomly amplified polymorphic DNAs markers. Asian-Australas J Anim Sci 18:453-457.   DOI
21 Jeffreys AJ, Morton DB (1987) DNA fingerprints of dogs and cats. Anim Genet 18:1-15.