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http://dx.doi.org/10.12717/DR.2017.21.1.035

Serum Cytokine Levels are related to Nesfatin-1/NUCB2 Expression in the Implantation Sites of Spontaneous Abortion Model of CBA/j×DBA/2 Mice  

Chung, Yiwa (Department of Bioenvironmental Technology, College of Natural Sciences, Seoul Women's University)
Kim, Heejeong (Department of Bioenvironmental Technology, College of Natural Sciences, Seoul Women's University)
Seon, Sojeong (Department of Bioenvironmental Technology, College of Natural Sciences, Seoul Women's University)
Yang, Hyunwon (Department of Bioenvironmental Technology, College of Natural Sciences, Seoul Women's University)
Publication Information
Development and Reproduction / v.21, no.1, 2017 , pp. 35-46 More about this Journal
Abstract
The process of spontaneous abortion involves a complex mechanism with various cytokines, growth factors, and hormones during the pregnancy. However, the mechanism underlying spontaneous abortion by pro- and anti-inflammatory cytokines in the serum during the pregnancy is not fully understood. Therefore, the purpose of this study was to examine the relationship between the serum levels of pro- and anti-inflammatory cytokines and spontaneous abortion using the $CBA/j{\times}DBA/2$ mouse model. Serum levels of pro-inflammatory cytokines, such as $IFN-{\gamma}$, $IL-1{\alpha}$ and $TNF-{\alpha}$ were not increased in abortion model mice, but anti-inflammatory cytokines, such as IL-4, IL-13 and IL-1ra were decreased compared to normal pregnant mice. In addition, serum levels of chemokine, such as SDF-1, G-CSF, M-CSF, IL-16, KC and MCP-1 were decreased in abortion model mice compared to normal pregnant mice. However, the expression levels of nesfatin-1/NUCB2 mRNA and protein in the uteri of implantation sites were significantly higher in abortion model mice than normal pregnant mice. These results suggest that uterine nesfatin-1/NUCB2 expression may be down-regulated by inflammatory cytokines and chemokines in the serum of pregnant mice. Moreover, this study suggests the possibility that nesfatin-1/NUCB2 expressed in the implantation sites may be associated with the maintenance of pregnancy.
Keywords
Nesfatin-1; Cytokine; Chemokine; Nesfatin-1/NUCB2; Spontaneous abortion;
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