Browse > Article
http://dx.doi.org/10.12717/DR.2013.17.4.451

Suppressive Effects of an Ishige okamurae extract on 3T3-L1 Preadipocyte Differentiation  

Cha, Sun-Yeong (Division of Developmental Biology and Physiology, School of Biosciences and Chemistry, Sungshin Women's University)
Cheon, Yong-Pil (Division of Developmental Biology and Physiology, School of Biosciences and Chemistry, Sungshin Women's University)
Publication Information
Development and Reproduction / v.17, no.4, 2013 , pp. 451-459 More about this Journal
Abstract
The biological activity of tissue specific stem cell is under the control of their specific microenvironment and the exogenous chemicals derived from digestive tract can be one of the constructing factors of that. It is suggested that the extract of brown algae Ishige okamurae has antioxidant-, apoptosis induction-, and antiinflammatory-effects. On the other hand, a few studies have shown that antioxidant assist inhibition of accumulation of fat. So we studied the effect of the extract of I. okamura on the cellular activity and differentiation of 3T3-L1 preadipocyte to adipose cell. The viability of cell was analyzed using 3-[4,5-dimethylthiazo-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. Adipogenesis of 3T3-L1 cell was analyzed after induction in the induction medium containing the I. okamurae extract. The cellular activity was high compared with the vehicle and 0.05 mM caffeine in all groups of I. okamurae extract treated cells. The extract of I. okamura inhibited accumulation of lipids in 10 and $50{\mu}g/ml$. The expression of the marker genes for adipocyte differentiation coincided with cytochemical results. These results suggest that the extract of I. okamurae increases the cellular viability of adipose precursor cells. On the other hand, it suppresses the differentiation of preadipocyte to adipocyte and accumulation of lipids in concentration-dependent manners. It may be possible that the major component of the extract can be applied in the control of adipose tissuegenesis.
Keywords
Extract of Ishige okamurae; 3T3-L1 preadipocyte; Adipogenesis; Viability; Differentiation;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Amer E, Westermark PO, Spalding KI, Britton T, Ryden M, Frisen J, Bernard S, Amer P (2010) Adpocyte turnover: relevance to human adipose tissue morphology. Diabetes 59:105-109.   DOI   ScienceOn
2 Fu J, Oveisi F, Gaetani S, Lin E, Piomelli D (2005b) Oleoylethanolamide, an endogenous PPAR-alpha agonist, lowers body weight and hyperlipidemia in obese rats. Neuropharmacology 48:1147-53.   DOI   ScienceOn
3 Gustafson B, Smith U (2012) The WNT inhibitor Dickkopf1 and bone morphogenetic protein 4 rescue adipogenesis in hypertrophic obesity in humans. Diabetes 61:1217- 1224.   DOI
4 Heo, SJ, Hwang JY, Choi JI, Han JS, Kim HJ, Jeon YJ (2009) Diphlorethohydroxycarmalol isolated from Ishige okamurae, a brown algae, a potent alphaglucosidase and alpha-amylase inhibitor, alleviates postprandial hyperglycemia in diabetic mice. Eur J Pharmacol 615:252-256.   DOI   ScienceOn
5 Jansson PA, Pellme F, Hammarstedt A, Sandqist M, Brekke H, Caidahl K, Forsberg M, olkmann R, Carvalho E, Funahashi T, Matsuzawa Y, Wiklund O, Yang X, Taskinen MR, Smith U (2003) A novel cellular marker of insulin resistance and early atherosclerosis in humans is related to impaired fat cell differentiation and low adiponectin. FASEB J 17:1434-1440.   DOI   ScienceOn
6 Park MH, Jeon YJ, Kim HJ, Han JS (2013b) Effect of diphlorethohydroxycarmalol isolated from Ishie okamurae on apoptosis in 3 T3-L1 preadipocytes. Phytother Res 27:931-936.   DOI   ScienceOn
7 Riedel A, Pignitter M, Hochkogler CM, Rohm B, Walker J, Bytof G, Lantz I, Somaza V (2012) Caffeine dosedependently induced thermogenesis but restores ATP in HepG2 cells in culture. Food Funct 3:955-964.   DOI   ScienceOn
8 Cha JY, Kim HJ, Yu JH, Xu J, Kim D, Paul DB, Choi H, Kim S, Lee YJ, Ho GP, Rao F, Snyder SH, Kim JW (2013) Dexras1 mediates glucocorticoid-associated adipgenesis and diet-induced obesity. Proc Natl Acad Sci U S A 110:20575-20580.   DOI   ScienceOn
9 Ross SE, Hemati N, Longo KA, Bennett CN, Lucas PC, Erickson RL, MacDougald OA (2000) Inhibition of adipogenesis by Wnt signaling. Science 289:950-953.   DOI   ScienceOn
10 Arner P, Arner E, Hammarstedt A, Smith U (2011) Geneti predisposition for type 2 diabetes, but not for overweight/ obesity, is associated with a restircted adipogenesis. Plos ONE 6:318284.
11 Chen H, Wang S, Chen L, Chen Y, Wu M, Zhang Y, Yu K, Huang Z, Qin L, Mo D (2013a) MicroRNA-344 inhibits 3T3-L1 cell differentiaiton via targeting GSK3$\beta$ of Wnt/$\beta$-catenin signaling pathway. FEBS Lett.
12 Chen Y, Xue P, Hou Y, Zhang H, Zheng H, Zhou T, Qu W, Teng W, Zhang Q, Andersen ME, Pi J (2013b) Isoniazid suppresses antioxidant response element activities and impairs adipogenesis in mouse and human preadipocytes. Toxicol Appl Pharmacol 273:435-441.   DOI   ScienceOn
13 Farmer SR (2006) Transcriptional control of adipocyte formation. Cell Metab 4:263-273.   DOI   ScienceOn
14 Farmer SR, Auwerx J (2004) Adipose tissue: new therapeutic targets from molecular and genetic studies- IASO Stock Conference 2003 report. Obes Rev 5: 189-196.   DOI   ScienceOn
15 Fruhbeck G, Gomez-Ambrosi J, Muruzabal FJ, Burrel MA (2001) The adipocyte: a model for integration of endocrine and metabolic signaling in energy metabolism regulation. Am J Physiol Endocrinol Metab 280:E827-47.
16 Ahn MJ, Yoon KD, Kim CY, Min SY, Kim Y, Kim HJ, Kim JH, Shin CG, Lee CK, Kim TG, Kim SH, Huh H, Kim J (2002) Inhibition of HIV-1 reverse transcriptase and HIV-1 integrase and antiviral activity of Korean seaweed extracts. J Appl Phycol 14:325- 329.   DOI   ScienceOn
17 Jeon Y, Song S, Kim H, Cheon YP (2013) Diphlorethohydroxycarmalol of Ishige okamurae and caffeine moidfied the expression of extracellular fibrillars during adipogenesis of mouse subcutaneous adipose derived stem cell. Dev Reprod 17:275-287.   DOI   ScienceOn
18 Kim MM, Rajapakse N, Kim SK (2009) Anti-inflammatory effect of Ishige okamurae ehtanolic extract via inhibition of NF-kappaB transcription factor in RAW 264.7 cells. Phytother Res 23:628-634.   DOI   ScienceOn
19 Kang SI, Shin HS, Kim HM, Yoon SA, Kang SW, Kim JH, Kim SJ (2012) Pepalonia binghamiae extract and its constituent fucoxanthin ameliorate high-fat diet-induced obesity by activating AMP-activated protein kinase. J Agric Food Chem 60:3389-3395.   DOI   ScienceOn
20 Kim KJ, Lee BY (2012) Fucoidan form the sporophyll of Undaria pinnatifida suppresses adipocyte differentiation by inhibiton of inflammation-related cytokines in 3T3- L1 cells. Nutr Res 32:439-447.   DOI   ScienceOn
21 Lai PY, Tsai CB, Tseng MJ (2013) Active form Notch4 promotes the proliferation and differentiation of 3T3-L1 preadipocytes. Biochem Biophys Res Commun 430: 1132-1139.   DOI   ScienceOn
22 Lee IK, Kang JW (1986) A check list of marine algae in Korea. Algae 1:311-325.
23 Lee WJ, Koh EH, Won JC, Kim MS, Park JY, Lee KU (2005) Obesity: the role of hypothalamic AMPactivated protein kinase in body weight regulation. Int J Biochem Cell Biol 37:2254-2259.   DOI   ScienceOn
24 Lefterova MJ, Lazar MA (2009) New developments in adipogenesis. Trends Endocrinol Metab 20:107-114.   DOI   ScienceOn
25 Muenzner M, Tuvia N, Deutschmann C, Witte N, Tolkachov A, Valai A, Henze A, Sander LE, Raila J, Schupp M (2013) Retinol-dinding protein 4 and its membrane receptor STRA6 control adipogenesis by regulating cellular retinoid homeostasis and retinoic acid receptor $\alpha$ activity. Mol Cell Biol 33:4068-4082.   DOI   ScienceOn
26 Nakabayashi H, Hashimoto T, Ashida H, Nishiumi S, Kanazawa K (2008) Inhibitory effects of caffeine and its metabolites on intracellular lipod accumulation in murine 3T3-L1 adipocytes. BioFactors 34:293-302.   DOI
27 Park J, Park J, Nahm SS, Choi I, Kim I (2013a) Identification of anti-adipogenic proteins in adult bovine serum suppressing 3T3-L1 preadipocyte differentiation. BMB Rep 46:582-587.   DOI   ScienceOn
28 Smas CM, Sul HS (1993) Pref-1, a protein containing EGF-like repeats, inhiits adipocyte differentiation. Cell 73:725-734.   DOI   ScienceOn
29 Seres I, Bajnok L, Harangi M, Sztanek F, Koncsos P, Paragh G (2010) Alteration of PON1 activity in adult and childhood obesity and its relation to adipokine levels. Adv Exp Med Biol 660:129-142.   DOI   ScienceOn
30 Shirosaki M, Koyama T (2011) Laminaria japonica as a food for the prevention of obesity and diatetes. Adv Food Nutr Res 64:199-212.   DOI   ScienceOn
31 Spalding KL, Arner E, Westermark PO, Bernard S, Buchholz BA, Bergmann O, Blomqvist L, Hoffstedt J, Naslund E, Britton T, Concha H, Hassan M, Ryden M, Frisen J, Arner P (2008) Dynamics of fat cell turnover in humans. Nature 453:783-787.   DOI   ScienceOn
32 Student AK, Hsu RY, Lane MD (1980) Induction of fatty acid synthetase in differentiating 3T3-L1 preadipocytes. J Biol Chem 255:4745-4750.
33 Tontonoz P, Hu E, Spiegelman BM (1994) Stimulation of adipogenesis in fibroblasts by PAR gamma 2, a lipid-activated transcription factor. Cell 79:1147-1156.   DOI   ScienceOn
34 Tontonoz P, Spiegelman M (2008) Fat and beyond: the diverse biology of PPARgamma. Annu Rev Biochem 77:289-312.   DOI   ScienceOn
35 Ullah M, Stich S, Haupl T, Eucker J, Sittinger M, Ringe J (2013) Reverse differentiation as a gene filtering tool in genome expression profiling of adipogenesis for fat marker gene selection and their analysis. PLOS One 8:e69754.   DOI
36 Xue P, Hou Y, Chen Y, Yang B, Fu J, Zheng H, Yarborough K, Woods CG, Liu D, Yamamoto M, Zhang Q, Andersen ME, Pi J (2013) Adipose dificinecy of Nrf2 in ob/ob mice results in severe metabolic syndrome. Diabetes 62:845-854.   DOI
37 Wang ZW, Pan WT, Lee Y, Kakuma T, Zhou YT, Unger RH (2001) The role of leptin resistance in the abnormalities of aging. FASEB J 15:108-114.   DOI   ScienceOn
38 Yeh WC, Cao Z, Classon M, McKnihgt SL (1995) Cascade regulation of terminal adipocyte differentiation by three members of the C/EBP family of leucine zipper proteins. Genes Dev 9:168-181.   DOI   ScienceOn
39 Fu Y, Luo N, Klein RL, Garvey WT (2005a) Adiponectin promotes adipocyte differentiation, insulin sensitivity, and lipid accumulation. J Lipid Res 46:1369-1379.   DOI   ScienceOn
40 Min KH, Kim HJ, Han JS (2011) Ishige okamurae ameliorates hyperglycemia and insulin resistance in C57BL/KsJ-db/db mice. Diabetes Res Clin Pract 93:70-76.   DOI   ScienceOn