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http://dx.doi.org/10.12717/DR.2013.17.1.017

Decreased Expression of Inhibitor of DNA-binding (Id) Proteins and Vascular Endothelial Growth Factor and Increased Apoptosis in Ovarian Aging  

Park, Min Jung (Center for Reproductive Medicine, Good Moonhwa Hospital)
Park, Sea Hee (Center for Reproductive Medicine, Good Moonhwa Hospital)
Moon, Sung Eun (Department of Obstetrics and Gynecology, Good Moonhwa Hospital)
Koo, Ja Seong (Department of Obstetrics and Gynecology, Good Moonhwa Hospital)
Moon, Hwa Sook (Department of Obstetrics and Gynecology, Good Moonhwa Hospital)
Joo, Bo Sun (Center for Reproductive Medicine, Good Moonhwa Hospital)
Publication Information
Development and Reproduction / v.17, no.1, 2013 , pp. 17-24 More about this Journal
Abstract
This study examined the expression of inhibitor of DNA-binding (Id) proteins and vascular endothelial growth factor (VEGF) in the ovary according to female age using a mice model as the first step in investigating the potential role of Ids and VEGF in ovarian aging. C57BL inbred female mice of three age groups (6-9, 14-16, and 23-26 weeks) were injected with 5 IU pregnant mare's serum gonadotropin (PMSG) in order to synchronize the estrus cycle. After 48 h, ovarian expression of Ids and VEGF was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR), western blot and immunohistochemistry. Ovarian apoptosis was examined by ovarian expression of Bcl-2 and Bcl-xL. Expression of Id-1 and VEGF was decreased with advancing female age, but not Id-2, Id-3, and Id-4. In particular, their expressions were significantly decreased in aged mice of 23-26 weeks compared with the young mice of 6-9 weeks (p < 0.05). In contrast, ovarian apoptosis was greatly increased in the aged mice compared to the young mice. This result suggests that Id-1 may have an implicated role in ovarian aging by associating with VEGF.
Keywords
Ovarian aging; Apoptosis; Id proteins; VEGF;
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