Browse > Article

KiSS1, KiSS2, GPR54 mRNA Expression of the Blacktip Grouper Epinephelus fasciatus  

Kang, Hyeong-Cheol (Marine and Environmental Research Institute of Jeju National University)
Lee, Chi-Hoon (Marine and Environmental Research Institute of Jeju National University)
Song, Young-Bo (Marine and Environmental Research Institute of Jeju National University)
Baek, Hea-Ja (Dept. of Marine Biology, Pukyong National University)
Kim, Hyung-Bae (Dept. of Marine Bio-resources, Gangwon Provincial University)
Lee, Young-Don (Marine and Environmental Research Institute of Jeju National University)
Publication Information
Development and Reproduction / v.16, no.2, 2012 , pp. 121-128 More about this Journal
Abstract
Kisspeptin has been reported to facilitate sexual maturation and ovulation by directly stimulating GnRH neurons via its receptor, GPR54. The KiSS-GPR54 system is playing an important role in the reproduction of several mammalian species. However, little is known about their function in fish. The aim of this study is to understand the physiological function and evolutionary conservation of KiSS-GPR54 system in teleost fish blacktip grouper, Epinephelus fasciatus. In the present study, we have partial cloned KiSS1, KiSS2 GPR54 mRNAs from a brain samples. Tissue distribution analysis using RT-PCR revealed that the KiSS1, KiSS2, GPR54 transcripts were expressed in different tissue. The KiSS-GPR54 system in gonadal of immature and mature stage were analyzed using qRT-PCR. The partial sequence of KiSS1, KiSS2, GPR54 were 232 bp, 304 bp, 613 bp long, respectively. KiSS1, KiSS2, GPR54 mRNAs are shown common expression in the brain. The amount of KiSS1, KiSS2 mRNAs expression were significantly higher in mature stage than immature stage. However GPR54 mRNA expression was higher in immature stage. These results are in good agreement with the hypothesis that KiSS-GPR54 system plays an important role in the regulation of reproductive function in the blacktip grouper.
Keywords
Blacktip grouper; KiSS1; KiSS2; GPR54;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Plant TM, Ramaswamy S, Dipietro MJ (2006) Repetitive administration of hypothalamic G protein-coupled receptor 54 with intravenous pulses of kisspeptin in the juvenile monkey (Macaca mulatta) elicits a sustained train of gonadotropin-releasing hormone discharges. Endocrinology 147:1007-1013.   DOI
2 Revel FG, Saboureau M, Masson-Pevet M, Pevet P, Mikkelsen JD, Simonneaux V (2006) Kisspeptin mediates the photoperiodic control of reproduction in hamsters. Curr Biol 16:1730-1735.   DOI   ScienceOn
3 Roa J, Vigo E, Castellano JM, Navarro VM, Fernandez- Fernandez R, Casanueva FF, Dieguez C, Aguilar E, Pinilla L, Tena-Sempere M (2006) Hypothalamic expression of KISS-1 system and gonadotropin releasing effects of kisspeptin in different reproductive states of the female rat. Endocrinology 147:2864-2878.   DOI   ScienceOn
4 Selvaraj S, Kitano H, Fujinaga Y, Ohga H, Yoneda M, Yamaguchi A, Shimizu A, Matsuyama M (2010) Molecular characterization, tissue distribution, and mRNA expression profiles of two Kiss genes in the adult male and female chub mackerel (Scomber japonicus) during different gonadal stages. Gen Comp Endocrinol 169:28-38.   DOI   ScienceOn
5 Lee DK, Nguyen T, O'Neill GP (1999) Discovery of a receptor related to galanin receptors. FEBS Lett 446:103-107.   DOI   ScienceOn
6 Messager S, Chatzidaki EE, Ma D, Hendrick AG, Zhan D, Dixon J, Thresher RR, Malinge I (2005) Kisspeptin directly stimulates gonadotropin-releasing hormone release via G protein coupled receptor 54. Proc Natl Acad Sci USA 102:1761-1766.   DOI   ScienceOn
7 Mohamed JS, Benninghoff AD, Holt GJ, Khan IA (2007) Developmental expression of the G protein-coupled receptor 54 and three GnRH mRNAs in the teleost fish cobia. J Mol Endocrinol 38:235-244.   DOI   ScienceOn
8 Muir AI, Chamberlain L, Elshourbagy NA (2001) AXOR12, a novel human G protein-coupled receptor. Nature 411:613-617.   DOI   ScienceOn
9 Nocillado JN, Levavi-Sivan B, Carrick F, Elizur A (2007) Temporal expression of G-protein-coupled receptor 54 (GPR54) gonadotropin-releasing hormones (GnRH), and dopamine receptor D2 (drd2) in pubertal female grey mullet, Mugil cephalus. Gen Comp Endocrinol 150:278-287.   DOI   ScienceOn
10 Othaki T, Shintani Y, Honda S, Matsumoto H, Hori A, Kanehashi K, Tearo Y, Kumano S, Takatsu Y, Masuda Y (2001) Metastasis suppressor gene KiSS-1 encode peptide ligand of a G protein-coupled receptor. Nature 411:613-617.   DOI   ScienceOn
11 Parhar IS, Ogawa S, Sakuma Y (2004) Laser-captured single digoxigenin-labeled neurons of gonadotropinreleasing hormone types reveal a novel g proteincoupled receptor (GPR54) during maturation in cichlid fish. Endocrinology 145:3613-3618.   DOI   ScienceOn
12 Han SK, Gottsch ML, Lee KJ, Popa SM, Smith JT, Jakawich SK, Clifton DK, Steiner RA, Herbison AE (2005) Activation of gonadotropin-releasing hormone neurons by kisseptin as a neuroendocrine switch for the onset of puberty. J Neurosci 25:11349-11356.   DOI   ScienceOn
13 Irwig MS, Fraley GS, Smith JT, Acohido BV, Popa SM, Cunningham MJ, Gottsch ML, Clifton DK, Steiner RA (2004) Kisspeptin activation of gonadotropin releasing hormone neurons and regulation of KiSS-1 mRNA in the male rat. Neuroendocrinology 80:264-272.   DOI   ScienceOn
14 Kanda S, Akazome Y, Matsunaga T, Yamamoto N, Yamada S, Tsukamura H, Maeda K-i, Oka Y (2008) Identification of KiSS-1 product kisspeptin and steroid sensitive sexually dimorphic kisspeptin neurons in medaka. Endocrinology 149:2467-2476.   DOI   ScienceOn
15 Karsch FJ, Dahl GE, Evans NP, Manning JM, Mayfield KP, Moenter SM (1993) Seasonal changes in gonadotropin- releasing hormone secretion in the ewe: alteration in response to the negative feedback action of estradiol. Biol Repro 49:1377-1383.   DOI   ScienceOn
16 Kawabe K, Kimura J (2007) Seedling production of blacktip grouper Epinephelus fasciatus in small tanks. Saibai Giken 35:11-21. (In Japanese)
17 Kitahashi T, Ogawa S, Parhar IS (2009) Cloning and expression of Kiss2 in the zebrafish and medaka. Neuroendocrinology 150:821-831.
18 Lee JH, Miele ME, Hicks DJ, Phillips KK, Trent JM, Weissman BE, Welch DR (1996) KiSS-1, a novel malignant melanoma metastasis-suppressor gene. J Natl Cancer Inst 88:1731-1737.   DOI
19 Kobayashi M, Amano M, Kim MH, Yoshiura Y, Sohn YC, Suetake H, Aida K (1997) Gonadotropin-releasing hormone and gonadotropin in goldfish and masu salmon. Fish Physiol Biochem 17:1-8.   DOI   ScienceOn
20 Lee CH, Hur SW, Song YB, Takano K, Takemura A, Lee YD (2010) Effect of 11-ketotestosterone (11-KT) on gonadal sex reversal and spermatogenesis of honeycomb grouper Epiephelus merra. Dev Reprod 14(1):1-5.
21 de Roux N, Genin E, Carel JC, Matsuda F, Chaussain JL, Milgrom E (2003) Hypogonadotropic hypogonadism due to loss of function in the KiSS1-derived peptide receptor GPR54. Proc Natl Acad Sci USA 100:10972-10976.   DOI   ScienceOn
22 Colledge WH (2004) GPR54 and puberty. Trends Endocrinol Metab 15:448-453.   DOI
23 Colledge WH (2009) Kisspeptins and GnRH neuronal signalling. Trends Endocrinol Metab 20:115-121.   DOI   ScienceOn
24 Dungan HM, Clifton DK, Steiner RA (2006) Kisspeptin neurons as central processors in the regulation of GnRH secretion. Endocrinology 147:1154-1158.   DOI
25 Felip A, Zanuy S, Pineda R, Pinilla L, Carrillo M, Tena- Sempere M, Gomez A (2009) Evidence for two distinct KiSS genes in non-placental vertebrates that encode kisspeptins with different gonadotropin-releasing activities in fish and mammals. Mol Cell Endocrinol 312:61-71.   DOI   ScienceOn
26 Funes S, Hendrick JA, Vassileva G, Markowitz L, Abbondanzo S, Golovko A, Yang S, Monsma FJ, Gustafson EL (2003) The KiSS-1 receptor GPR54 is essential for the development of the murine reproductive system. Biochem Biophys Res Commun 312: 1357-1363.   DOI   ScienceOn
27 Seminara SB, Messager S, Chatzidaki EE, Thresher RR, Acierno JS, Shagoury JK, Bo-Abbas Y, Kuohung W, Schwinof KM, Hendrick AG (2003) The GPR54 gene as a regulator of puberty. N Engl J Med 349:1614-1627.   DOI   ScienceOn
28 Goos HJT, Facchinetti F, Henderson IW, Pierantoni R, Polzonetti-Magni AM (1993) Pubertal development: big questions, small answers. Cell Comm Repord 11-20.
29 Gottsch ML, Cunningham MJ, Smith JT, Popa SM, Acohido BV, Crowley WF, Seminara S, Clifton DK, Steiner RA (2004) A role for kisspeptinsin the regulation of gonadotropin secretion in the mouse. Endocrinology 145:4073-4077.   DOI   ScienceOn
30 Greives TJ, Mason AO, Scott, MAL, Levine J, Ketterson ED, Kriegsfeld LJ, Demas GE (2007) Environmental control of kisspeptin: implications for seasonal reproduction. Endocrinology 148:1158-1166.   DOI
31 Shahab M, Mastronardi C, Seminara SB, Crowley WF, Ojeda SR, Plant TM (2005) Increased hypothalamic GPR54 signaling: a potential mechanism for initiation of puberty in primates. Proc Natl Acad Sci USA 102: 2129-2134.   DOI   ScienceOn
32 Shahjahan M, Motohashi E, Doi H, Ando H (2010) Elevation of Kiss2 and its receptor gene expression in the brain and pituitary of grass puffer during the spawning season. Gen Comp Endocrinol 169:48-57.   DOI   ScienceOn
33 Shimizu Y, Tomikawa J, Hirano K, Nanikawa Y, Akazome Y, Kanda S, Kazeto Yi, Okuzawa K, Uenoyama Y, Ohkura S, Tsukamura H, Maeda K-i, Gen K, Oka Y, Yamamoto N (2012) Central distribution of kiss2 neurons and peri-pubertal changes in their expression in the brain of male and female red seabream Pagrus major. Gen Comp Endocrinol 175:432-442.   DOI   ScienceOn
34 Smith JT, Dungan HM, Stoll EA, Gottsch ML, Braun RE, Eacker SM, Clifton DK, Steiner RA (2005) Differential regulation of KiSS-1 mRNA expression by sex steroids in the brain of the male mouse. Endocrinology 146:2976-2984.   DOI   ScienceOn
35 Yang B, Jiang Q, Chan T, Ko WKW, Wong AOL (2010) Goldfish kisspeptin: Molecular cloning, tissue distribution of transcript expression, and stimulatory effects on prolactin, growth hormone and luteinizing hormone secretion and gene expression via direct actions at the pituitary level. Gen Comp Endocrinol 165:60-71.   DOI   ScienceOn
36 Song YB, Oh SR, Seo JP, Ji BG, Lim BS, Lee YD (2005) Larval development and rearing of longtooth grouper Epinephelus bruneus in Jeju Island, Korea. J World Aquacult Soc 36: 209-216.
37 Tena-Sempere M. Huhtaniemi I (2003) Gonadotropins and gonadotropin receptors. In: Fauser BCJM (ed.), Reproductive Medicine-Molecular, Cellular and Genetic Fundamentals. Parthenon Publishing, New York, pp 225-244.
38 Thompson EL, Patterson M, Murphy KG, Smith KL, Dhillo WS, Todd JF, Ghatei MA, Bloom SR (2004) Central and peripheral administration of kisspeptin-10 stimulates the hypothalamo-pituitary-gonadal axis. J Neuroendocrinol 16:850-858.   DOI   ScienceOn
39 Mechaly AS, Vinas J, Piferrer F (2009) Identification of two isoforms of the Kisspeptin-1 receptor (kiss1r) Generated by Alternative splicing in a Modern Teleost, the senegalese sole (Solea senegalensis). Biol Reprod 80:60-69.   DOI   ScienceOn