Browse > Article

Downstream Genes Regulated by Bcl2l10 RNAi in the Mouse Oocytes  

Kim, Eun-Ah (Dept. of Biomedical Science, College of Life Science, CHA University, CHA Research Institute, Fertility Center, CHA General Hospital)
Kim, Kyeoung-Hwa (Dept. of Biomedical Science, College of Life Science, CHA University, CHA Research Institute, Fertility Center, CHA General Hospital)
Lee, Hyun-Seo (Dept. of Biomedical Science, College of Life Science, CHA University, CHA Research Institute, Fertility Center, CHA General Hospital)
Lee, Su-Yeon (Dept. of Biomedical Science, College of Life Science, CHA University, CHA Research Institute, Fertility Center, CHA General Hospital)
Kim, Eun-Young (Dept. of Biomedical Science, College of Life Science, CHA University, CHA Research Institute, Fertility Center, CHA General Hospital)
Seo, You-Mi (Dept. of Biomedical Science, College of Life Science, CHA University, CHA Research Institute, Fertility Center, CHA General Hospital)
Bae, Jee-Hyeon (Dept. of Pharmaceuticals, CHA University)
Lee, Kyung-Ah (Dept. of Biomedical Science, College of Life Science, CHA University, CHA Research Institute, Fertility Center, CHA General Hospital)
Publication Information
Development and Reproduction / v.15, no.1, 2011 , pp. 61-69 More about this Journal
Abstract
Previously, we have shown that Bcl2l10 as a member of Bcl-2 family, key regulators of the apoptotic process, is dominantly expressed in oocytes of ovary but several member of the Bcl-2 family are not expressed in oocytes. Recent our studies had been processed about roles and regulatory mechanisms of Bcl2l10 in oocytes. Microinjection of Bcl2l10 RNAi into the cytoplasm of germinal vesicle oocytes resulted in metaphase I (MI) arrest and exhibited abnormalities in their spindles and chromosome configurations (Yoon et al., 2009). The present study was conducted to elucidate the downstream genes regulated by Bcl2l10 and signaling networks in Bcl2l10 RNAi microinjected oocytes by using microarray analysis. Surprisingly, we found that a large proportion of genes regulated by Bcl2l10 RNAi were involved in the cell cycle and actin skeletal system regulation as important upstream genes of Bcl2l10. Among the transcripts with highly significant fold changes more than 2-fold, Tpx2 and Cep192 are 16.1- and 8.2-fold down regulated respectively by Bcl2l10 RNAi. Tpx2 and Cep192 are known as cofactors that control Aurora A kinase activity and localization. Therefore, we concluded that Bcl2l10 may have important roles during oocyte meiosis as functional upstream regulator of Tpx2 and Cep192.
Keywords
Bcl2l10; RNAi; Microarray; Spindle; Tpx2;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Yoon SJ, Kim EY, Kim YS, Lee HS, Kim KH, Bae J, Lee KA (2009) Role of Bcl2-like 10 (Bcl2110) in regulating mouse oocyte maturation. Biol Reprod 81:497-506.   DOI   ScienceOn
2 Lu PJ, Lu QL, Rughetti A, Taylor-Papadimitriou J (1995) Bcl-2 overexpression inhibits cell death and promotes the morphogenesis, but not tumorigenesis of human mammary epithelial cells. J Cell Biol 129:1363-1378.   DOI   ScienceOn
3 Naumann U, Weit S, Wischhusen J, Weller M (2001) Diva/Boo is a negative regulator of cell death in human glioma cells. FEBS Lett 505:23-26.   DOI   ScienceOn
4 Ozlu N, Srayko M, Kinoshita K, Habermann B, O'Toole ET, Muller-Reichert T, Schmalz N, Desai A, Hyman AA (2005) An essential function of the C. elegans ortholog of TPX2 is to localize activated aurora A kinase to mitotic spindles. Dev Cell 9:237-248.   DOI   ScienceOn
5 Song Q, Kuang Y, Dixit VM, Vincenz C (1999) Boo, a novel negative regulator of cell death, interacts with Apaf-1. EMBO J 18:167-178.   DOI
6 Tsai MY, Wiese C, Cao K, Martin O, Donovan P, Ruderman J, Prigent C, Zheng Y (2003) A Ran signalling pathway mediated by the mitotic kinase Aurora A in spindle assembly. Nat Cell Biol 5:242-248.   DOI   ScienceOn
7 Inohara N, Gourley TS, Carrio R, Muniz M, Merino J, Garcia I, Koseki T, Hu Y, Ches S, Nunez G (1998) Diva, a Bcl-2 homologue that binds directly to Apaf-1 and induces BH3-independent cell death. J Biol Chem 273:32479-32486.   DOI
8 Joukov V (2011) Aurora kinases and spindle assembly: variations on a common theme? Cell Cycle 10:895-903.   DOI   ScienceOn
9 Kim JH, Yoon S, Won M, Sim SH, Ko JJ, Han S, Lee KA, Lee K, Bae J (2009) HIP1R interacts with a member of Bcl-2 family, BCL2L10, and induces BAK-dependent cell death. Cell Physiol Biochem 23:43-52.   DOI   ScienceOn
10 Kufer TA, Sillje HH, Korner R, Gruss OJ, Meraldi P, Nigg EA (2002) Human TPX2 is required for targeting Aurora-A kinase to the spindle. J Cell Biol 158:617-623.   DOI   ScienceOn
11 Adams JM, Cory S (1998) The Bcl-2 protein family: arbiters of cell survival. Science 281:1322-1326.   DOI   ScienceOn
12 Barr AR, Gergely F (2007) Aurora-A: the maker and breaker of spindle poles. J Cell Sci 120:2987-2996.   DOI   ScienceOn
13 Wu MY, Tsai TF, Beaudet AL (2006) Deficiency of Rbbpl/Arid4a and Rbbp111/Arid4b alters epigenetic modifications and suppresses an imprinting defect in the PWS/AS domain. Genes Dev 15:2859-2870.
14 Xu X, Wang X, Xiao Z, Li Y, Wang Y (2011) Two TPX2-dependent swithches control the activity of Aurora A. PLoS One 6:e16757.   DOI
15 Yoon SJ, Kim JW, Choi KH, Lee SH, Lee KA (2006) Identification of oocyte-specific diva-associated proteins using mass spectrometry. Kor J of Fertil Steril 33:189-198.
16 Bayliss R, Sardon T, Vernos I, Conti E (2003) Structural basis of Aurora-A activation by TPX2 at the mitotic spindle. Mol Cell 12:851-862.   DOI   ScienceOn