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Methylation Patterns of Imprinting Genes, H19, Igf2r, and Snrpn, in Mouse Embryonic Stem Cells and Nuclear Transferred Embryonic Stem Cells  

Lee, Min-Ho (Genetics Laboratory, Fertility Center of CHA Gangnam Medical Center, CHA University)
Ju, Jin-Young (Dept. of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine)
Cho, Youl-Hee (Dept. of Medical Genetics, College of Medicine, Hanyang University)
Shim, Sung-Han (Genetics Laboratory, Fertility Center of CHA Gangnam Medical Center, CHA University)
Publication Information
Development and Reproduction / v.14, no.4, 2010 , pp. 253-259 More about this Journal
Abstract
DNA methylation is one of the major epigenetic regulations of gene expression. The DNA methylation patterns are dramatically changed during gametogenesis and embryogenesis, and especially, it has been known that embryonic stem cells show a distinct methylation pattern. In this study, we examined the methylation patterns of imprinting genes, H19, Igf2r, and Snrpn, in stem cells induced from fertilized embryo (fES) and somatic cell nuclear transferred embryo (ntES). The methylation pattern of H19 gene in both fES and ntES were similar. However, the methylation patterns of Igf2r and Snrpn in ntES (hypermethylated) were slightly different from fES cells.
Keywords
Imprinting genes; Methylation pattern; Embryonic stem cells;
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