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Characterization of Human Thigh Adipose-derived Stem Cells  

Heo, Jin-Yeong (Dept. of Biotechnology, College of Natural Science, Seoul Women's University)
Yoon, Jin-Ah (Dept. of Biotechnology, College of Natural Science, Seoul Women's University)
Kang, Hyun-Mi (Dept. of Biotechnology, College of Natural Science, Seoul Women's University)
Park, Se-Ah (Dept. of Biotechnology, College of Natural Science, Seoul Women's University)
Kim, Hae-Kwon (Dept. of Biotechnology, College of Natural Science, Seoul Women's University)
Publication Information
Development and Reproduction / v.14, no.4, 2010 , pp. 233-241 More about this Journal
Abstract
Human adipose stem cells are an abundant, readily available population of multipotent progenitor cells that reside in adipose tissue and these cells have characteristics very similar to bone marrow mesenchymal stromal cells (BMMSCs). However, liposuction procedure, donor age, body mass index, and harvesting sites might generate differences in the initial cell population and the preparations are a heterogeneous mixture of precursors with different subsets. Therefore, in this study, we investigated the characteristics of human thigh adipose stem cells and the differentiation potential into mesodermal and endodermal lineage. Thigh adipose stem cells maintained fibroblast-like morphology similar to BM-MSCs and they underwent average 56.5 doublings and produced $5{\times}10^{22}$ cells. These cells expressed SCF, Oct4, nanog, vimentin, CK18, FGF5, NCAM, Pax6, BMP4, HNF4a, nestin, GATA4, HLA-ABC, and HLA-DR genes at p3 and they also expressed Oct4, Thy-1, FSP, vWF, vimentin, desmin, CK18, CD54, CD4, CD106, CD31, a-SMA, HLA-ABC proteins. Moreover, they could differentiate into mesodermal lineage cells such as adipocyte, osteoblast and chondrocyte. In addition, they also differentiated into insulin secreting cells in our culture condition. In conclusion, human thigh adipose stem cells retain proliferative potential and expression patterns similar to BM-MSCs and they also differentiate into various cell types. Thus, human thigh adipose stem cells might be useful alternative cell source for clinical application.
Keywords
Thigh adipose stem cells; Characterization; Insulin-secreting cells; Differentiation potential;
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1 Kang HM, Kim J, Park S, Kim J, Kim H, Kim KS, Lee EJ, Seo SI, Kang SG, Lee JE, Lim H (2009) Insulin-secreting cells from human eyelid-derived stem cells alleviate type I diabetes in immunocompetent mice. Stem Cells 27:1999-2008.   DOI   ScienceOn
2 Tankó LB, Bagger YZ, Alexandersen P, Larsen PJ, Christiansen C (2003) Central and peripheral fat mass have contrasting effects on the progression of aortic calcification in postmenopausal women. Eur Heart J 24:1531-1537.   DOI   ScienceOn
3 Woodbury D, Schwarz EJ, Prockop DJ, Black IB (2000) Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res 61:364-370.   DOI   ScienceOn
4 Wu X, Hoffstedt J, Deeb W, Singh R, Sedkova N, Zilbering A, Zhu L, Park PK, Arner P, Goldstein BJ (2001) Depotspecific variation in protein-tyrosine phosphatase activities in human omental and subcutaneous adipose tissue: A potential contribution to differential insulin sensitivity. J Clin Endocrinol Metab 86:5973-5980.   DOI   ScienceOn
5 Xu W, Zhang X, Qian H, Zhu W, Sun X, Hu J, Zhou H, Chen Y (2004) Mesenchymal stem cells from adult human bone marrow differentiate into a cardiomyocyte phenotype in vitro. Exp Biol Med 229:623-631.   DOI
6 Van Harmelen V, Rohrig K, Hauner H (2004) Comparison of proliferation and differentiation capacity of human adipocyte precursor cells from the omental and subcutaneous adipose tissue depot of obese subjects. Metabolism 53:632-637.   DOI   ScienceOn
7 Zuk PA, Zhu M, Mizuno H, Huang JI, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH (2001) Multilineage cells from human adipose tissue: Implications for cellbased therapies. Tissue Eng 7:211-228.   DOI   ScienceOn
8 Strem BM, Hicok KC, Zhu M, Wulur I, Alfonso Z, Schreiber RE (2005) Multilineage differentiation of adipose tissue-derived stem cells. Keio J Med 54:132-141.   DOI   ScienceOn
9 Tchkonia T, Giorgadze N, Pirtskhalava T, Tchoukalova Y, Karagiannides I, Forse RA, DePonte M, Stevenson M, Guo W, Han J, Waloga G, Lash TL, Jensen MD, Kirkland JL (2002) Fat depot origin affects adipogenesis in primary cultured and cloned human preadipocytes. Am J Physiol Regul Integr Comp Physiol 282:1286-1296.   DOI
10 Timper K, Seboek D, Eberhardt M, Linscheid P, Christ-Crain M, Keller U, Müller B, Zulewski H (2006) Human adipose tissue-derived mesenchymal stem cells differentiate into insulin, somatostatin, and glucagon expressing cells. Biochem Biophys Res Commun 341:1135-1140.   DOI   ScienceOn
11 Jurgens WJ, Oedayrajsingh-Varma MJ, Helder MN, Zandiehdoulabi B, Schouten TE, Kuik DJ (2008) Effect of tissue harvesting site on yield of stem cells derived from adipose tissue: Implications for cell-based therapies. Folia Histochem Cytobiol 46:307-314.   DOI   ScienceOn
12 Lee KD, Kuo TK, Whang-Peng J, Chung YF, Lin CT, Chou SH, Chen JR, Chen YP, Lee OK (2004) In vitro hepatic differentiation of human mesenchymal stem cells. Hepatology 40:1275-1284.   DOI   ScienceOn
13 Oedayrajsingh-Varma MJ, van Ham SM, Knippenberg M, Helder MN, Klein-Nulend J, Schouten TE (2006) Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure. Cytotherapy 8:166-177.   DOI   ScienceOn
14 Stenderup K, Justesen J, Clausen C, Kassem M (2003) Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells. Bone 33:919-926.   DOI   ScienceOn
15 Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284:143-147.   DOI   ScienceOn
16 Sotiropoulou PA, Perez SA, Salagianni M, Baxevanis CN, Papamichail M (2006) Chracterization of the optimal culture conditions for clinical scale production of human mesenchymal stem cells. Stem Cells 24:462-471.   DOI   ScienceOn
17 Sowers JR (2003) Obesity as a cardiovascular risk factor. Am J Med 115:37-41.   DOI   ScienceOn
18 Bacou F, el Andalousi RB, Daussin PA, Micallef JP, Levin JM, Chammas M, Casteilla L, Reyne Y, Nougues J (2004) Transplantation of adipose tissue-derived stromal cells increases mass and functional capacity of damaged skeletal muscle. Cell Transplant 13:103-111.   DOI
19 Cowan CM, Shi YY, Aalami OO, Chou YF, Mari C, Thomas R, Quarto N, Contag CH, Wu B, Longaker MT (2004) Adipose-derived adult stromal cells heal critical-size mouse calvarial defects. Nat Biotechnol 22:560-567.   DOI   ScienceOn
20 Baxter MA, Wynn RF, Jowitt SN, Wraith JE, Fairbairn LJ, Bellantuono I (2004) Study of telomere length reveals rapid aging of human marrow stromal cells following in vitro expansion. Stem Cells 22:675-682.   DOI   ScienceOn
21 Fraser JK, Wulur I, Alfonso Z, Zhu M, Wheeler ES (2007) Differences in stem and progenitor cell yield in different subcutaneous adipose tissue depots. Cytotherapy 9:459-467.   DOI   ScienceOn
22 Gimble JM, Guilak F (2003) Differential potential of adipose derived adult stem (ADAS) cells. Curr Topics Dev Biol 58:137-160.   DOI
23 Gronthos S, Franklin DM, Leddy HA, Robey PG, Storms RW, Gimble JM (2001) Surface protein characterization of human adipose-derived stromal cells. J Cell Physiol 189:54-63.   DOI   ScienceOn
24 Hicok KC, Du Laney TV, Zhou YS, Halvorsen YD, Hitt DC, Cooper LF, Gimble JM (2004) Human adipose-de-rived adult stem cells produce osteoid in vivo. Tissue Eng 10:371-380.   DOI   ScienceOn
25 Ho AD, Wagner W, Franke W (2008) Heterogeneity of mesenchymal stromal cell preparations. Cytotherapy 10:320-330.   DOI   ScienceOn