Browse > Article
http://dx.doi.org/10.7742/jksr.2022.16.5.559

A Rat Pylorus Stricture Model to Create Stent-induced Granulation Tissue Formation  

Kim, Min-Tae (Department of Radiologic Technology, Cheju Halla University)
Publication Information
Journal of the Korean Society of Radiology / v.16, no.5, 2022 , pp. 559-565 More about this Journal
Abstract
In this study, we intend to develop a granulation tissue formation model. As a pilot experiment, a contrast agent was injected into the pylorus in 3 rats, the normal pylorus lumen size was confirmed, and a stent was placed. Stent migration was confirmed in to the duodenum within 1 week. In this experiment, stent was sutured and fixed to induce granulation tissue formation after gastrostomy under a fluoroscopic guidance. Twenty rats were divided into Healthy Group / Gastrostomy Group. After anesthesia of the Gastrostomy Group, an abdominal incision was performed, and gastrostomy was performed under a fluoroscopic guidance, and a stent was placed into the pylorus. In order to prevent stent migration due to peristalsis, suture between the pylorus and the proximal end of the stent was performed. Postoperative behavior and weight changes were monitored daily. Four weeks after surgery, gastrointestinal fluoroscopy imaging was performed and rats were sacrifices. To evaluate the degree of granulation formation, the stent was sectioned transversely. Gastrostomy group was statistically significantly higher than Healthy Group in granulation area ratio (all p<.001). In conclusion, it is considered that the level of tissue overgrowth formation for preclinical evaluation of the pylorus stricture model through gastrostomy is appropriate as a research evaluation tool.
Keywords
Gastrostomy; pylorus stricture. stent; granulation tisssue formation; preclinical model;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 E. Y. Kim, H. Y. Song, J. C. Kim, Y. S. Yoon, B. D. Ye, D. H. Nam, S. J. Shin, "Mmp-9 expression after metallic stent placement in patients with colorectal cancer: association with in-stent restenosis", Radiology, Vol. 271, No. 3, pp. 901-909, 2014. http://dx.doi.org/10.1148/radiol.13121794   DOI
2 J. E. van Hooft, M. L. van Montfoort, S. M. Jeurnink, M. J. Bruno, M. G. Dijkgraaf, P. D. Siersema, P. Fockens, "Safety and efficacy of a new non-foreshortening nitinol stent in malignant gastric outlet obstruction (DUONITI study): a prospective, multicenter study", Endoscopy, Vol. 43, No. 8, pp. 671-675, 2011. https://doi.org/10.1055/s-0030-1256383   DOI
3 Y. C. Heo, D K. Han,, M. T. Kim, "Therapeutic effect of local photothermal heating of gold nanoparticle-coated self-expandable metallic stents for suppressing granulation tissue formation in the mouse colon", PLOS ONE, Vol. 16, No. 4, pp. e0249530, 2021. http://dx.doi.org/10.1371/journal.pone.0249530   DOI
4 K. Y. Kim, J. H. Park, D. H. Kim, J. W. Tsauo, M. T. Kim, W. C. Son, S. G. Kang, D. H. Kim, H. Y. Song, "Sirolimus-eluting Biodegradable Poly-l-Lactic Acid Stent to Suppress Granulation Tissue Formation in the Rat Urethra", Radiology, Vol. 286, No. 1, pp. 140-148, 2018. http://dx.doi.org/10.1148/radiol.2017170414   DOI
5 E. J. Jun, J. H. Park, J. Tsauo, S. G. Yang, D. K. Kim, K. Y. Kim, M. T. Kim, S. H. Yoon, Y. J. Lim, H. Y. Song, "EW-7197, an activin-like kinase 5 inhibitor, suppresses granulation tissue after stent placement in rat esophagus", Gastrointestinal Endoscopy, Vol. 86, No. 1, pp. 219-228, 2017. https://doi.org/10.1016/j.gie.2017.01.013   DOI
6 T. M. Geiger, B. W. Miedema, Z. Tsereteli, E. Sporn, K. Thaler, "Stent placement for benign colonic stenosis: case report, review of the literature, and animal pilot data", International journal of colorectal disease, Vol. 23, No. 10, pp. 1007-1012, 2008. http://dx.doi.org/10.1007/s00384-008-0518-9   DOI
7 C. Brasselet, E. Durand, F. Addad, F. Vitry, G. Chatellier, C. Demerens, M. Lemitre, R. Garnotel, D. Urbain, P. Bruneval, A. Lafont, "Effect of local heating on restenosis and in-stent neointimal hyperplasia in the atherosclerotic rabbit model: a dose-ranging study", European Heart Journal, Vol. 29, No. 3, pp. 402-412, 2008. http://dx.doi.org/10.1093/eurheartj/ehm596   DOI
8 Y. Wu, L. Chen, P. G. Scott, E. E. Tredget, "Mesenchymal stem cells enhance wound healing through differentiation and angiogenesis", Stem cells (Dayton, Ohio), Vol. 25, No. 10, pp. 2648-2659, 2007. http://dx.doi.org/10.1634/stemcells.2007-0226   DOI
9 M. T. Kim, "A Mouse Colon Model to Investigate Stent-Induced Tissue Hyperplasia", Journal of the Korean Society of Radiology, Vol. 14, No. 4, pp. 439-445, 2020. http://dx.doi.org/http://dx.doi.org/10.7742/jksr.2020.14.4.439   DOI
10 E. M. L. Verschuur, M. Y. V. Homs, E. W. Steyerberg, J. Haringsma, P. J. Wahab, E. J. Kuipers, P. D. Siersema, "A new esophageal stent design (Niti-S stent) for the prevention of migration: a prospective study in 42 patients", Gastrointestinal Endoscopy, Vol. 63, No. 1, pp. 134-140, 2006. http://dx.doi.org/10.1016/j.gie.2005.07.051   DOI
11 J. Li, J. Chen, R. Kirsner, "Pathophysiology of acute wound healing", Clinics in Dermatology, Vol. 25, No. 1, pp. 9-18, 2007. https://doi.org/10.1016/j.clindermatol.2006.09.007   DOI
12 E. E. van Halsema, P. Fockens, J. E. van Hooft, "Palliation of Gastric Outlet Obstruction", Clinical Gastrointestinal Endoscopy, pp. 367-373, 2019. https://doi.org/10.1016/B978-0-323-41509-5.00033-5   DOI
13 J. H. Kim, H. Y. Song, J. H. Shin, E. Choi, T. W. Kim, H. Y. Jung, G. H. Lee, S. K. Lee, M. H. Kim, M. H. Ryu, Y. K. Kang, B. S. Kim, J. H. Yook, "Metallic stent placement in the palliative treatment of malignant gastroduodenal obstructions: prospective evaluation of results and factors influencing outcome in 213 patients", Gastrointestinal Endoscopy, Vol. 66, No. 2, pp. 256-264, 2007. http://dx.doi.org/10.1016/j.gie.2006.12.017   DOI
14 L. Li, R. Wang, H. H. Shi, L. Xie, J. D. S. Li, W. C. Kong, J. T. Tang, D. N. Ke, L. Y. Zhao, "In vitro study on the feasibility of magnetic stent hyperthermia for the treatment of cardiovascular restenosis", Experimental and Therapeutic Medicine, Vol. 6, No. 2, pp. 347-354, 2013. http://dx.doi.org/10.3892/etm.2013.1177   DOI
15 J. E. Lopera, A. Brazzini, A. Gonzales, W. R. Castaneda-Zuniga, "Gastroduodenal Stent Placement: Current Status", Radiographics :a review publication of the Radiological Society of North America, Inc., Vol. 24, No. 6, pp. 1561-1574, 2004. http://dx.doi.org/10.1148/rg.246045033   DOI
16 J. S. Ji, B. I. Lee, H. K. Kim, Y. S. Cho, H. Choi, B. W. Kim, S. W. Kim, S. S. Kim, H. S. Chae, K. Y. Choi, L. S. Maeng, "Antimigration property of a newly designed covered metal stent for esophageal stricture: an in vivo animal study", Gastrointestinal Endoscopy, Vol. 74, No. 1, pp. 148-153, 2011. http://dx.doi.org/10.1016/j.gie.2011.03.1252   DOI
17 J. H. Kim, H. Y. Song, J. H. Park, H. J. Yoon, H. G. Park, D. K. Kim, "IN-1233, an ALK-5 inhibitor: prevention of granulation tissue formation after bare metallic stent placement in a rat urethral model", Radiology, Vol. 255, No. 1, pp. 75-82, 2010. http://dx.doi.org/10.1148/radiol.09090670   DOI
18 R. F. Diegelmann, M. C. Evans, "Wound healing: an overview of acute, fibrotic and delayed healing", Frontiers in Bioscience (Landmark Edition), Vol. 9, pp. 283-9, 2004. http://dx.doi.org/10.2741/1184   DOI
19 F. Arnold, D. C. West, "Angiogenesis in wound healing", Pharmacology and Therapeutics, Vol. 52, No. 3, pp. 407-422, 1991. http://dx.doi.org/10.1016/0163-7258(91)90034-J   DOI
20 M. C. Zhang, N. Cresswell, F. Tavora, E. Mont, Z. Zhao, A. Burke, "In-stent restenosis is associated with neointimal angiogenesis and macrophage infiltrates", Pathology Research and Practice, Vol. 210, No. 12, pp. 1026-30, 2014. http://dx.doi.org/10.1016/j.prp.2014.04.004   DOI