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http://dx.doi.org/10.17946/JRST.2022.45.2.165

Photodynamic Inactivation of Staphylococcus Aureus Based on Dose of Laser Transmission  

Koo, Bon-Yeoul (Department of Radiology, Vision College of Jeonju)
Kim, Ji-Won (Department of Radiological Science, Jeonju University)
Publication Information
Journal of radiological science and technology / v.45, no.2, 2022 , pp. 165-170 More about this Journal
Abstract
Staphylococcus aureus is a major pathogen that causes clinical infections in humans and can also cause massively colonized in lesion skin, particularly in atopic dermatitis patients. This study investigated the effects of photodynamic inactivation with radachlorin and diode laser irradiation on the viability of S. aureus in vitro and assessed the effects of the dose of laser transmission. In the PDI group, 5 𝜇L of S. aureus suspension and 5 𝜇L of radachlorin were inoculated in a 55 mm petri dish (63.6 cm2). The samples were placed in a 37° incubator for 30 min and then irradiated with light (660 nm diode laser). After laser irradiation, the cells were stored for 24 h at 37° in an incubator with 5% CO2, and the number of colonies was counted. All CFU/mL of S. aureus were reduced by diode laser in the presence of radachlorin, with a killing rate of 87.9% at an energy dose of 9 J/cm2. This study contribute to treat colonized with S. aureus in atopic dermatitis patients and wound infections by providing information on the optimal dose of laser transmission using PDI to eliminate S. aureus.
Keywords
Photodynamic inactivation; Staphylococcus aureus; Laser dose; Photosensitizer; Radaclorin;
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Times Cited By KSCI : 3  (Citation Analysis)
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