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http://dx.doi.org/10.3857/jkstro.2010.28.4.219

In vivo Radiosensitization Effect of H DAC Inhibitor, SK-7041 on RIF-1 Cell Line  

Chie, Eui-Kyu (Department of Radiation Oncology, Seoul National University College of Medicine)
Shin, Jin-Hee (Department of Radiation Oncology, Seoul National University College of Medicine)
Kim, In-Ah (Department of Radiation Oncology, Seoul National University College of Medicine)
Kim, Il-Han (Department of Radiation Oncology, Seoul National University College of Medicine)
Publication Information
Radiation Oncology Journal / v.28, no.4, 2010 , pp. 219-223 More about this Journal
Abstract
Purpose: To test the radiosensitizing effect of the newly synthesized novel histone deacetylase inhibitor, SK-7041 in vivo. Materials and Method: The RIF-l cell line was implanted into the back of a 6-week-old female C3H mouse, intradermally, The mice were grouped into control, drug, radiation (RT), and RT+drug group. SK-7041, 4 mg/kg was administered intraperitoneally for six cycles every 12 hours for mice in the drug and RT+drug group, An identical volume of phosphate buffered saline (PBS) was administered at the same frequency to mice in the control and RT groups. A single 5 Gy fraction was delivered to mice in RT and RT+drug group 6 hours after the fourth delivery. The volume of the implanted tumor was measured every 2~3 days to formulate the growth delay curve. Results: For the control, drug, RT, and RT +drug groups, the average duration for implanted tumor to reach a volume of $1,500mm^3$ was 10 days, 10 days, 9 days, and 12 days, respectively. Moreover, the tumor volume on D14 was $276.7mm^3$, $279.9mm^3$, $292.5mm^3$, and $185.5mm^3$, respectively (p=0.0004). The difference for the change in slope for the control and drug versus the RT and RT+drug groups were borderline significant (p=0.0650). Conclusion: The results of this study indicate that SK-7041 has a radiosensitizing effect for the RIF-1 cell line in vivo at a low concentration and this effect may be synergistic. Implementing this result to clinical trial is warranted.
Keywords
Radiation; Histone deacetylase inhibitor; SK-7041; in vivo;
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