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http://dx.doi.org/10.3857/jkstro.2010.28.4.211

Catalase Induced by All-Trans Retinoic Acid Is Involved in Antiproliferation of 36B10 Cells  

Park, Woo-Yoon (Department of Radiation Oncology, Chungbuk National University College of Medicine)
Yu, Jae-Ran (Department of Environmental and Tropical Medicine, Konkuk University College of Medicine)
Publication Information
Radiation Oncology Journal / v.28, no.4, 2010 , pp. 211-218 More about this Journal
Abstract
Purpose: All-trans retinoic acid (ATRA) has anti proliferative effects against brain tumor cells. Recently, ATRA has been reported to induce catalase. We investigated whether catalase induction by ATRA is associated with its anti proliferative effects. Materials and Methods: 36B10 cells were exposed to 0~50${\mu}M$ ATRA for 24 or 48 hours and mRNA, protein, and activity of catalase were measured. Reactive oxygen species (ROS) were measured using 2',7'-dichlorofluorescin diacetate. A clonogenic assay was used to confirm the cytotoxic effect. Results: The mRNA, protein, and activity of catalase were found to increase in a concentration- and incubationtime-dependent manner. The increase in catalase activity induced by ATRA was decreased by the addition of 3-amino-1,2,4-triazole (ATZ). ROS was also increased with ATRA and decreased by the addition of ATZ. The decrease in cell survival induced by ATRA was partly rescued by ATZ. Conclusion: Catalase induction by ATRA is involved in ROS overproduction and thus inhibits the proliferation of 36B10 cells.
Keywords
All-trans retinoic acid; Catalase; Reactive oxygen species;
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