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Effects of Recombinant Human Epidermal Growth Factor on the Proliferationand Radiation Survival of Human Fibroblast Cell Lines in Vitro  

Kim, Hyun-Sook (Department of Radiology, Gyeongsang National University College of Medicine)
Kang, Ki-Mun (Department of Radiation Oncology, Gyeongsang National University College of Medicine)
Lee, Sang-Wook (Department of Radiation Oncology, University of Ulsan College of Medicine, Asan Medical Center)
Na, Jae-Boem (Department of Radiology, Gyeongsang National University College of Medicine)
Chai, Gyu-Young (Department of Radiation Oncology, Gyeongsang National University College of Medicine)
Publication Information
Radiation Oncology Journal / v.24, no.3, 2006 , pp. 179-184 More about this Journal
Abstract
[ $\underline{Purpose}$ ]: To explore the effect of recombinant human EGF on the proliferation and survival of human fibroblast cell lines following irradiation. $\underline{Materials\;and\;Methods}$: Fibroblast was originated human skin and primary cultured. The trypan blue stain assay and MTT assay were used to study the proliferative effects of EGF on human fibroblast cell lines in vitro. An incubation of fibroblasts with rhEGF for 24 hours immediately after irradiation was counted everyday. Cell cycle distributions were analyzed by FACS analysis. $\underline{Results}$: Number of fibroblast was significantly more increased rhEGF (1.0 nM, 10 nM, 100 nM, 1,000 nM) treated cell than control after 8 Gy irradiation. Most effective dose of rhEGF was at 160 nM. These survival differences were maintained at 1 week later. Proportion of S phase was significantly increased on rhEGF treated cells. $\underline{Conclusion}$: rhEGF cause increased fibroblast proliferation following irradiation. We expect that rhEGF was effective for radiation induced wound healing.
Keywords
Recombinant human epidermal growth factor; Radiation; Fibroblast cell;
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