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The Effect of Gingko Biloba Extract on Energy Metabolic Status in C3H Mouse Fibrosarcoma : Evaluated by in vivo $^{31}P$ Magnetic Resonance Spectroscopy  

Ha Sung Whan (Department of Therapeutic Radiology, Laboratory of Radiation Biology, Cancer Research Center, Seoul National University, College of Medicine)
Kim Won Dong (Department of Therapeutic Radiology, Laboratory of Radiation Biology, Cancer Research Center, Seoul National University, College of Medicine)
Ahn Yong Chan (Department of Therapeutic Radiology, Laboratory of Radiation Biology, Cancer Research Center, Seoul National University, College of Medicine)
Park Chan Il (Department of Therapeutic Radiology, Laboratory of Radiation Biology, Cancer Research Center, Seoul National University, College of Medicine)
Lim Tae Hwan (NMR Division, Asan Institute for Life Sciences, Ulsan University)
Lee Tae Kuen (NMR Division, Asan Institute for Life Sciences, Ulsan University)
Publication Information
Radiation Oncology Journal / v.20, no.2, 2002 , pp. 147-154 More about this Journal
Abstract
Purpose : Gingko biloba extract (GBE), a natural product extracted from Gingko leaves, is known to increase the radiosensitivity of tumors. This radiosensitization probably arises from the increase in the peripheral blood flow by decreasing the blood viscosity and relaxing the vasospasm. The influence of a GBE on the metabolic status in fibrosarcoma II (FSall) of a C3H mouse was investigated using $^{31}P$ magnetic resonance spectroscopy (MRS). Materials and Methods : Eighteen C3H mice with fibrosarcoma II $(from\;100\;mm^3\;to\;130\;mm^3)$ were prepared for this experiment. The mice were divided into 2 groups; one (9 mice) without a priming dose, and the other (9 mice) with a priming dose of GBE. The GBE priming dose (100 mg/kg) was administered by an intraperitoneal (i.p.) injection 24 hours prior to the measurement. First $^{31}P$ MRS spectra were measured in the mice from each group as a baseline and test dose of GBE (100 mg/kg) was then administered to each group. One hour later, the $^{31}P$ MRS spectra were measured again to evaluate the change in the energy metabolic status. Results : In the group without the priming dose, the mean pH, PCr/Pi, PME/ATP, Pi/ATP, PCr/(Pi+PME) values 1 hour after the test dose were not changed significantly compared to the values at the baseline. However, in the group with the priming dose, the mean PCr/Pi, Pi/ATP, PCr/(Pi+PME) values 1 hour after the test dose changed from the baseline values of 0.49, 0.77, 0.17 to 0.74, 0.57, 0.28 respectively. According to the paired t-test, the differences were statistically significant. Conclusion : The above findings suggest that the metabolic status is significantly improved after administering GBE if the priming dose is given 24 hours earlier. This shows that the radiosensitizing effect of GBE is based on the increase of tumor blood flow and the improvement in the metabolic status.
Keywords
Gingko biloba extract; Mouse fibrosarcoma; Energy metabolism; $^{31}P$ magnetic resonance spectroscopy;
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