Browse > Article
http://dx.doi.org/10.14480/JM.2021.19.3.256

Optimization of protoplast isolation and PEG-mediated transformation in Agaricus bisporus  

Kim, Minseek (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
Jang, Kab-yeul (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
Lee, Yun-Sang (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
Oh, Min Ji (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
Im, Ji-Hoon (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
Oh, Youn-Lee (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
Publication Information
Journal of Mushroom / v.19, no.3, 2021 , pp. 256-259 More about this Journal
Abstract
Currently, button mushroom, Agaricus bisporus is one of the most consumed mushroom in the world. However, despite of its importance in food market, molecular genetic modification method for breeding of A. bisporus is not well established. In this study, we optimized yield of A. bisporus protoplast with Lysing enzyme, Chimax-N and cellulase. With this composition, 1.0 × 108/mL of protoplasts were obtained reliably. PEG-mediated transformation with spermidine showed almost 100-fold higher yield than non-spermidine method.
Keywords
Agaricus bisporus; protoplast isolation; PEG transformation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Gang Li, Ruixue Li, Qiuyun Liu, Qiang Wang, Min Chen, Baojian Li. 2006. A highly efficient polyethylene glycol-mediated transformation method for mushrooms. FEMS Microbiol Lett 256(2): 203-208   DOI
2 Chen X, Stone M, Schlagnhaufer C, Romaine CP. 2000. A Fruiting Body Tissue Method for Efficient Agrobacterium-Mediated Transformation of Agaricus bisporus. Appl Environ Microbiol 66(10): 4510-4513.   DOI
3 Oh YL, Jang KY, Oh MJ, Im JH. 2021. Development of a spawning method using liquid inoculum of Agaricus bisporus. J mushroom 19(2): 109-113   DOI
4 Sonnenberg AS, Baars JJ, Gao W, Visser RG. 2017. Developments in breeding of Agaricus bisporus var. bisporus: progress made and technical and legal hurdles to take. Appl Microbiol Biotechnol 101(5): 1819-1829.   DOI
5 Kim H, Kim ST, Ryu J, Choi MK, Kweon J, Kang BC, Ahn HM, Bae S, Kim JS, Kim SG. 2016 A simple, flexible and high-throughput cloning system for plant genome editing via CRISPR-Cas system. J Integr Plant Biol 58(8): 705-712   DOI
6 Royse DJ, Baars J, Tan Q. 2017. Current Overview of Mushroom Production in the World. Edible and Medicinal Mushrooms: Technology and Applications 5-13.
7 MAFRA. 2020. Ministry of Agriculture, Food and Rural Affairs 2019. 64-67.