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http://dx.doi.org/10.14480/JM.2014.12.3.181

Genetic variation of local varieties and mutants groups induced by gamma ray in Hypsizigus marmoreus  

Kim, Jong-Bong (Department of Medical Life Science, Catholic University of Daegu)
Yu, Dong-Won (Department of Medical Life Science, Catholic University of Daegu)
Publication Information
Journal of Mushroom / v.12, no.3, 2014 , pp. 181-186 More about this Journal
Abstract
This research was carried out to analyze the genetic variation of 18 wild strain, 2 breed varieties and 20 mutants of Hypsizygus marmoreus by random amplification of polymorphic DNA(RAPD). Also, 3 strains of Lyophyllum decartes and 1 strain of Lyophyllum shimeji were used. These mushrooms were collected from korea, china, Taiwan and Japan. Spores of H. marmoreus JV-2 strain were irradiated by gamma ray for mutagenesis. 40 kind of primers were used for this reaserch. Number of reaction primer were 31. Electrophorectic patterns of RAPD showed genetic variation. In phylogenetic tree, they were divided into seven group. Discriminative differences were observed between wild strain and mutants in H. marmoreus. These results might suggest that these primers and gamma ray irradiation of spores were useful tools for developing new strain for mushroom.
Keywords
Gamma ray; Genetic variation; Hypsizygus marmoreus; RAPD;
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Times Cited By KSCI : 6  (Citation Analysis)
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