Browse > Article

The Effects of MRI on Mouse Embryos During Fetal Stage  

Nakamura, Takashi (Graduate School of Health Science, Suzuka University of Medical Science)
Ryu, Myung-Sun (Graduate School of Health Science, Suzuka University of Medical Science)
Sakazaki, Takahiko (Graduate School of Health Science, Suzuka University of Medical Science)
Itokawa, Yuka (Graduate School of Health Science, Suzuka University of Medical Science)
Maenaka, Toshihiro (Graduate School of Health Science, Suzuka University of Medical Science)
Masubuchi, Takashi (Graduate School of Health Science, Suzuka University of Medical Science)
Sekimoto, Hiroyuki (Graduate School of Health Science, Suzuka University of Medical Science)
Kanehara, Masayuki (Graduate School of Health Science, Suzuka University of Medical Science)
Kang, Young-Nam (Graduate School of Health Science, Suzuka University of Medical Science)
Gu, Yeun-Hwa (Graduate School of Health Science, Suzuka University of Medical Science)
Publication Information
Journal of Radiation Protection and Research / v.31, no.2, 2006 , pp. 91-96 More about this Journal
Abstract
The effects of Magnetic resonance imaging (MRI) on mouse embryos at the early stage of organogenesis were investigated. Pregnant ICR mice were exposed on day 8 of gestation to MRI at 0.5 T for 0.5 hour to 3 hours. The mortality rates of embryos or fetuses, the incidence of external malformations, fetal body weight and sex ratio were observed at day 18 of gestation. A significant increase in embryonic mortality was observed after exposure to either 0.5 T MRI for 0.5 hour or 2 hours. However, the exposure to MRI for 1 hour or 3 hours did not induce any significant increase in embryonic mortality when compared with control. External malformations such as exencephaly, cleft palate and anomalies of tail were observed in all experimental groups exposed to each MRI. A statistically significant increase of external malformations was observed in all groups treated with 0.5 T MRI for 0.5 hour and 3 hours. The incidence of external malformations in the mice group exposed to 0.5 T MRI for 0.5-hour was found to be higher than those of mice group exposed to 0.5 T MRI for 2 hours. The effects of MRI on the external malformations might not to be dose-dependent. There was no statistically significant difference in fetal body weight and sex ratio among each MRI exposure groups.
Keywords
Embryonic effects; Organogenesis; Malformation; Magnetic Resonance Imaging(MRI);
Citations & Related Records
연도 인용수 순위
  • Reference
1 Murakami J, Torii Y, Masuda K. Fetal development of mice following intrauterine exposure to a static magnetic field of 6.3 T. Magn. Reson. Imaging. 1992;10:433-437   DOI   ScienceOn
2 Haseman JK, Hogan MD. Selection of the experimental unit in teratology studies. Teratology 1975;12:165-172   DOI   ScienceOn
3 Nishikawa U. Biological effects of pulsing electromagnetic field (PEMFs) on ICR mice. Nippon Seikeigeka Gakkai Zasshi 1987;61:1413-1428
4 Tyndall DA. MRI effects on craniofacial size and crown-rump length in C57BL/6J mice in 1.5 T fields. Oral Surg. Oral Med. Oral Pathol. 1993;76:655-660   DOI   ScienceOn
5 Tyndall DA. MRI effects on the teratogenicity of x-irradiation in the C57BL/6J mouse. Magn. Reson. Imaging 1990;8:423-433   DOI   ScienceOn
6 Kusama T, Yoshizawa Y. The synergistic effects of radiation and caffeine on embryonic development in mice. J. Radiat. Res. 1984;25:225-233   DOI
7 Carnes KI, Magin RL. Effects of in utero exposure to 4.7 T MR imaging conditions on fetal growth and testicular development in the mouse. Magn. Reson. Imaging 1996;14:263-274   DOI   ScienceOn
8 Kusama T, Hasegawa Y. Stage differences in developmental disorders in ICR mouse embryos irradiated with gamma rays. Cong. Anom. 1993;33:115-123   DOI
9 Carney AL. Magnetic resonance imaging (MRI): is it safe? Clin Electroencephalogr XI, 1989;20:12-43
10 Kowalczuk CI, Robbins L, Thomas JM, Butland BK. Saunders, Effects of prenatal exposure to 50 Hz magnetic fields on development in mice: I. Implantation rate and fetal development. Bio. Elec. Magnet. 1994;15:349- 361
11 Gu YH, Kai M, Kusama T. The embryonic and fetal effects in ICR mice irradiated in the various stages of the preimplantation period. Radiat. Res. 1997;147:735-740   DOI
12 Gu YH, Hasegawa T, Suzuki T. Combined effects of radiation and ultrasound on ICR mice in the preimplantation stage. Ultrasound Med. Biol. 2002;28(6):831-836   DOI   ScienceOn
13 Streffer C, Molls M. Cultures of preimplantation mouse embryos: A model or radiobiological studies. Adv. Radiat. Biol. 1987;13:169-212
14 Mevissen M, Buntenkotter S, Loscher W. Effects of static and time-arying (50-Hz) magnetic fields on reproduction and fetal development in rats. Teratology 1994;50:229-237   DOI   ScienceOn
15 Wilson JG, Fraser FC. Current status of teratology - General principles and mechanisms derived from animal studies. In Handbook of Teratology. General principles and etiology. Plenum Press 1979;1:47-74
16 Miyakoshi J, Ohtsu S, Shibata T, Takebe H. Exposure to magnetic field (5 mT at 60Hz) does not affect cell growth and c-myc gene expression. J. Radiat. Res. 1996;37:185-191   DOI
17 Heinrichs WL, Fong P, Flannery M, Heinrichs SC, Crooks LE, Spindle A, Pedersen RA. Midgestational exposure of pregnant BALB/c mice to magnetic resonance imaging conditions. Magn. Reson. Imaging. 1988;6:305-313   DOI   ScienceOn
18 Zimmermann B, Hentschel D. Effect of a static magnetic field (3.5 T) on the reproductive behavior of mice, on the embryo and fetal development and on selected hematologic parameters. Digitale Bilddiagn 1987;7:155-161
19 Kusama T, Sugiura N, Kai M, Yoshizawa Y. Effects of radiation and caffeine on embryonic development in mice. Radiat. Res. 1989;117:273-281   DOI
20 Konermann G, Monig H. Effect of static magnetic fields on the prenatal development of the mouse, Radiology 1986;26:490-497
21 ICRP; The recommendations of International Commission on Radiological Protection. Nonstochastic effects of ionizing radiation. Publ.41, Pergamon Press, Oxford. 1984: 1-26
22 Streffer C. Distribution of micronuclei among single cells of pre-implantation mouse embryos after X-irradiation invitro. Cell. Tissue. Kinet. 1980;13:135-143
23 Tyndall DA, Sulik KK. Effects of magnetic resonance imaging on eye development in the C57BL/6J mouse. Teratology 1991;43:263-275   DOI   ScienceOn
24 Gu YH. The development effects of Radiation on mice embryos in preimplantation stage. J. Kor. Associ. Radiat. Prote. 1996;21:273-284   과학기술학회마을
25 Kusama T, Gu YH. Effects of radiation and ultrasound on embryonic development in ICR mice. J. Radiat. Res. 1992;33:67-68
26 Okuda T, Nishizawa K, Ejima Y, Nakatsugawa S, Ishigaki T, Ishizaki K. The effects of static magnetic fields and X-rays on instability of microsatellite repetitive sequences. J. Radiat. Res. 1998;39:279-287   DOI