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Protective Effects of Paeonia japonica against Radiation-induced Damage  

Oh, Heon (Radiation Food Technology and Bioscience team, Korea Atomic Energy Research Institute)
Park, Hae-Ran (Radiation Food Technology and Bioscience team, Korea Atomic Energy Research Institute)
Jeong, Ill-Yun (Radiation Food Technology and Bioscience team, Korea Atomic Energy Research Institute)
Kim, Sung-Ho (College of Veterinary medicine, Chonnam National University)
Jo, Sung-Kee (Radiation Food Technology and Bioscience team, Korea Atomic Energy Research Institute)
Publication Information
Journal of Radiation Protection and Research / v.27, no.3, 2002 , pp. 181-188 More about this Journal
Abstract
We investigated the effect of Paeonia japonica (PJ) on radiation-induced oxidative damage to macromolecules in vitro and in vivo. The PJ reduced the tail moment (TM) which was a marker of DNA strand break in single-cell gel electrophoresis (SCGE; comet assay) in the human peripheral blood lymphocytes. Lipid peroxidation in the liver of the ICR mouse, measured as malondialdehyde (MDA), was also reduced by PJ administration. Ethanol fraction of PJ was more effective than polysaccharide fraction of that on reduction of TM in SCGE and lipid peroxidation. Also, Their activities to scavenge DPPH radicals and hydroxyl radicals were observed in vitro, and the activities were due to its ethanol fraction. It is plausible that scavenging of flee radicals by PJ extract may have played an important role in providing the protection against the radiation-induced damage. These results indicated that Paeonia japonica might be a useful radioprotector, especially since it is a relatively nontoxic natural product.
Keywords
Paeonia japonica; radioprotector; single-cell gel electrophoresis(SCGE: comet assay); lipid peroxidation; radical;
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  • Reference
1 NCP report No. 65 'Mamagement of persons Accidentally Contaminated with Radionuclides' P.77 (1980)
2 방면호, 송정춘, 이상양, 박남규, 백냥인 '작약 뿌리로부터 항산화활성 물질의 분리' 한국농화학회지, 42, 170-175 (1999)
3 IAEA safety series No. 47 'Manual on Early Medical Treatment of . Possible Radiation Injury' lAEA, Vienna, P.74 (1978)
4 Neta, R. 'Role of cytokines in radioprotection' Pharmacol. Ther., 39, 261-266 (1988)
5 Wang Y. and Zhu B. 'The effect of angelica polysaccharide on proliferation and differentiation of hematopoietic progenitor cell' Chung Hua I Hsueh Tsa Chih 76, 363-366 (1996)
6 Egger K. and Keil M. 'Flavone glycoside in the flowers of Paeonia arborea., P. suffruicosa' Planta., 88, 154-156 (1969)
7 문진영 ‘작약 약침액이 tert-butyl hydroperoxide로 유도된 흰쥐 배양 간세포의 지질과산화반응 및 항산화효소 활성에 마치는 영향’ 대한 침구학회지, 17, 176-187 (2000)
8 Cairnie AB. 'Adverse effect of radioprotector WR 2721' Radiat. Res., 94, 221-226 (1983)
9 Kim S.H., Cho C.K., Yoo S.Y., Koh K.H., Yun H.G. and Kim T.H. 'In vivo radioprotective activity of Panax ginseng and diethyldithiocarbarnate' IN VIVO, 7, 467-470 (1993)
10 한대석, ‘생약학’ 동명사, (1995)
11 Booth V.K., Roberts J.C, Warters R.L., Wilmore B.H. and Lepock J.R. 'Radioprotective thiolamines WR-1065 and WR-33278 selectively denature nonhistone nuclear proteins' Radiat. Res., 153, 813-822 (2000)
12 Gutteridge J.M. 'Reactivity of hydroxyl and hydroxyl-like radicals discriminated by release of thiobarbituric acid-reactive material from deoxy sugars, nucleosides and benzoate' Biochem. J., 224, 761-767 (1984)
13 Wang H.B., Zheng Q.Y., Ju D.W. and Fang J. 'Effects of Phytolacoa acinosa polysaccharides II on lymphocyte proliferation and colony stimulating factor production from mice splenocytes in vitro' Yao Hsueh Hsueh Pao, 28, 490-493 (1993)
14 Ohta S., Sakurai N., Sato Y., Inoue T. and Shinoda M. 'Studies on chemical protectors against radiation. XXX. Radioprotective substances of cnidii rhizoma' Yakugaku Zasshi, 110, 746-754 (1990)
15 Patt, H., Tyree, M. and Straube, R. L. 'Cystein protects against x-irradiation' Science, 110, 213-214 (1949)
16 Blois M.S. 'Antioxidant determination by the use of a stable free redical' Nature, 26, 1199-1200 (1958)
17 MacVittie, T. J., Monroy, R. L., Patchen, M. L. and Souza, L. M. 'Therapeutic use of recombinant human G-CSF (rhG-CSF) in a canine model of sublethal and lethal whole body irradiation' Int. J. Radiat. Biol., 57, 723-736 (1990)
18 Miyanomae T. and Frindel E. 'Radioprotection of hemopoiesis conferred by Acanthopanax senticosus Harms (Shigoka) administered before or after irradiation' Exp. Hematol., 16, 801-806 (1988)   PUBMED
19 Quan H.X. and Li H.S. 'Effects of radix Astragali on hemopoiesis in irradiated mice' Chung Kuo Chung Yao Tsa Chih, 19, 741-743 (1994)   PUBMED
20 Washburn LC, Carlton JE, Hayes RL. 'Distribution of WR-2721 in normal and malignant tissue of mice and rats bearing solid tumors: Dependence on turner type, drug dose and species' Radiat. Res., 59, 483-575 (1974)
21 Yuan Y, Hou S, Lian T and Han Y. 'Studies of Rehmannia glutinosa Libosch f. hueichingensis as a blood tonic' Chung Kuo Chung Yao Tsa Chih, 17: 366-368 (1992)
22 Lu G., Yang M., Shen Y. and Meng J. 'The absorption of Fe, Zn, Cu in siwu, sijunzi, and Liuwei dihuang decoction by smail intestine in rats' Chung Kuo Chung Yao Tsa Chih, 16, 297-298 (1991)
23 임창수, 김갑성 '작약 약침의 항산화 효능에 미치는 영향' 대한침구학회지, 16, 269-286 (1999)
24 Mei Q.B., Tao T.Y. and Cui B. 'Advances in the pharmacological studies of radix Angelica sinensis(Oliv) Diels (Chinese Danggui)' Chin Med J Engl, 104, 776-781 (1991)
25 Hanson W.R. 'Radiation protection of murine intestine by WR-2721, 16, 16-dimethyl prostaglandin $E_2$ and the combination of both agents' Radiat. Res., 111, 361-373, (1987)
26 Aimi N., Inaba M., Watanabe M. and Shibata S. 'Chemical studies on the oriental plant drugs. XXIII. Paeoniflorin, a glucoside of Chinese paeony root' Tertahedron., 25, 1825-1838 (1969)
27 Ohkawa H., Ohnishi N. and Yagi K 'Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction' Anal. Biochem, 95, 351-358 (1979)
28 한약위원회, 조제지침연구소위원회 : 한약조제지침서 해설, 사단법인 대한약사회 (1995)
29 Soka T. 'Encyclopedia of Chinese Medicine' vol. 3. 2066-2070. Tokyo, Japan, 1985
30 Milas, L., Hunter, N. Reid, B. O. and Thames, Jr. H. D. 'Protective effects of S-2-(3-aminopropylamino) ethylphosphorothioic acid against radiation damage of normal tissues and a fibrosarcoma in mice' Cancer Res., 42, 1888-1987 (1982)
31 Zneg X.L., Li X.A. and Zhang B.Y. 'Immunological and hematopoietic effect of Codonopsis pilosula on cancer patients during radiotherapy' Chung Kuo Chung Hsi I Chieh Ho Tsa Chih, 12, 607-608 (1992)
32 Hsu H.Y., Lian S.L. and Lin CC. 'Radioprotective effect of Ganoderma lucidum (Leyss. ex. Fr) Karst after X-ray irradiation in mice' Am J. Chin Med, 18, 61-69 (1990)
33 Okubo T., Nagai F., Seto T., Satoh K., Ushiyama K. and Kano I. 'The inhibition of phenylhydroquinone-induced oxidative DNA cleavage by constituents of Moutan Cortex and Paeoniae Radix' Biol. Pharm Bull., 23, 199-203 (2000)
34 Singh N.P., Stephens R.E. and Schneider E.L. 'Induction of DNA single- strand breaks in human lymphocytes by low doses of $\gamma$-ray' Int. J. Radiat Biol., 66, 563-569 (1995)
35 Li N.Q. 'Clinical and experimental study on shen-qi injection with chemotherapy in the treatment of malignant tumor of digestive tract' Chung Kuo Chung Hsi I Chieh Ho Tsa Chih, 12, 588-592 (1992)
36 Kligerman, M. M., Shaw. M. T., Slavid, M. and Yudas, J. M. 'Phase I clinical studies with WR2721' Cancer Clin. Trials, 3, 217-221 (1980)