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http://dx.doi.org/10.15204/jkobgy.2021.34.4.012

Inhibitory Effects of Cheongpochukeo-tang on LPS-induced Inflammation Model  

Hong, Ka-Kyung (Dept. of OB&GY, College of Oriental Medicine, Wonkwang University)
Lee, Soo-Hyung (Dept. of OB&GY, College of Oriental Medicine, Wonkwang University)
Jung, Hyun-Tae (Dept. of OB&GY, College of Oriental Medicine, Wonkwang University)
Kim, Song-Baek (Dept. of OB&GY, College of Oriental Medicine, Wonkwang University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.34, no.4, 2021 , pp. 12-29 More about this Journal
Abstract
Objective: This study was performed to investigate the inhibitory effect of Cheongpochukeo-tang (CCT) on lipopolysaccharide (LPS)-induced inflammation model. Methods: RAW 264.7 cells were pre-treated with CCT and incubated with LPS (500 ng/ml) after 1 hour. Cell viability was measured by MTT assay to figure out cytotoxicity of CCT. The production of nitric oxide and mRNA expression of pro-inflammatory cytokine were measured. And the activation of mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NF-κB) were examined to figure out molecular mechanisms of CCT's anti-inflammatory effects. In addition, mice survival rate and cytokine levels of serum were observed after treated with CCT. And mice liver tissues were observed and their cytokines levels in liver tissue were measured. Results: CCT did not have cytotoxic effect in RAW 264.7 cells. It inhibited LPS-induced nitric oxide (NO) production, but showed an increase in NO by itself at 2 mg/ml concentration. CCT inhibited mRNA expression of IL-1β, IL-6, TNF-α in a dose dependant and the activaton of MAPKs and NF-κB. In addition, CCT reduced mortality in the LPS-induced mouse model and inhibited production of cytokines in mouse serum and liver tissue. Conclusion: The results suggest that CCT could reduce LPS-induced inflammation by inhibiting MAPKs and NF-κB activaton, NO production, and pro-inflammatory cytokines secretion. Thereby, CCT could be effective medicine for the inflammatory disease.
Keywords
Cheongpochukeo-tang; Inflammation; Lipopolysaccharide (LPS); RAW 264.7 cell; Cytokines;
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1 Borrelli E, et al. Plasma concentrations of cytokines, their soluble receptors, and antioxidant vitamins can predict the development of multiple organ failure in patients at risk. Critical care medicine. 1996;24(3):392-7.   DOI
2 Cobb MH, Goldsmith EJ. Dimerization in MAP-kinase signaling. Trends Biochem Sci. 2000;25(1):7-9.   DOI
3 Cargnello M, Philippe PR. Activation and function of the MAPKs and their substrates, the MAPK-activated protein kinases. Microbiology and molecular biology reviews. 2011;75(1):50-83.   DOI
4 Lee JE. Drug Delivery Systems - associated with Pediatric Endocrinology. Journal of Korean Society of Pediatric Endocrinology. 2011;16(1):7-12.   DOI
5 Su GL. Lipopolysaccharides in liver injury: molecular mechanisms of Kupffer cell activation. American Journal of Physiology-Gastrointestinal and Liver Physiology. 2002;283(2):256-65.
6 Loeffler S, et al. Interleukin-6 induces transcriptional activation of vascular endothelial growth factor (VEGF) in astrocytes in vivo and regulates VEGF promoter activity in glioblastoma cells via direct interaction between STAT3 and Sp1. International journal of cancer. 2005;115(2):202-13.   DOI
7 Chung JH, Song BK. Effects of Kyokhach'ugotang and Ch'onp'och'ugotang on anti-inflammatory, antipyretic, analgesic, anti-microbial and anti-thrombose action. Kyunghee Univ. Theses collection. 1986;15:101-16.
8 Choi CM, et al. Analysis of Korean traditional medicinal treatment trend of infertility and development of questionnaire for infertility treatment. The Journal of Oriental Obstetrics & Gynecology. 2016;29(2):29-46.   DOI
9 Yun YG. Donguibangje and Cheobanghaeseol(東醫方劑와 處方解說). 4th rev. ed. Seoul: Euiseongdang. 2011:288-9.
10 Ryu IH, et al. Effects of Patrinia scabiosaefolia aqueous extract on cytokine and NF-κB activation in LPS-induced RAW 264.7 cells and mouse. The Journal of Oriental Obstetrics & Gynecology. 2017;30(2):1-15.   DOI
11 Lee EJ, et al. Inhibition of LPS-induced inflammatory biomarkers by ethyl acetate fraction of Patrinia scabiosaefolia through suppression of NF- κB activation in RAW 264.7 cells. Immunopharmacology and Immunotoxicology. 2012;34(2):282-91.   DOI
12 Westrom L, et al. Pelvic inflammatory disease and fertility. A cohort study of 1,844 women with laparoscopically verified disease and 657 control women with normal laparoscopic results. Sex Transm Dis. 1992;19(4):185-92.   DOI
13 Lee D, et al. Inhibition of Lipopolysaccharide-Inducible Nitric Oxide Synthase, TNF- α, IL-1β and COX-2 Expression by Flower and Whole Plant of Lonicera japonica. Journal of Physiology & Pathology in Korean Medicine. 2005;19(2):481-9.
14 Chae KW. Anti-inflammatory activity and osteoclast formation effects of Lonicerae Flos. Department of Herbology, Graduate school of Kyunghee university. 2008.
15 Yun KJ, Lee EY. Effects of hot aqueous and ethanol extract from Lonicera japonica Flos on NO and PGE2 in macrophage. Journal of Korean Acupuncture & Moxibustion Society. 2012;29(1):67-74.
16 Joeng JE, et al. In: Research proposal on the clinical cause and support plan of infertility. Ilsan:NHIS Ilsan Hospital Institute of Health Insurance & Clinical Research. 2017:51.
17 Spiller F, et al. Targeting nitric oxide as a key modulator of sepsis, arthritis and pain. Nitric Oxide. 2019;89(1):32-40.   DOI
18 Yun YG, et al. Inhibitory Effect of Aqueous Extract from Lonicera japonica Flower on LPS-induced Inflammatory Mediators in RAW 264.7 Macrophages. The Korea Journal of Herbology. 2007;22(3):117-25.
19 Kotowicz B, et al. The value of tumor marker and cytokine analysis for the assessment of regional lymph node status in cervical cancer patients. International Journal of Gynecologic Cancer. 2008;18(6):1279-84.   DOI
20 Tsan MF, Gao B. Pathogen-associated molecular pattern contamination as putative endogenous ligands of Toll-like receptors. Journal of endotoxin research. 2007;13(1):6-14.   DOI
21 Gang SH, Choi SH, Ahn KS. Experimental studies on effects of Qingbaozhuyutang(淸胞逐瘀湯), bee veenom on the blood stasis pattern. The journal of Korean oriental medicine society. 1995;16(1):351-69.
22 Baeuerle PA. I kappa B-NF-kappa B structures: at the interface of inflammation control. Cell. 1998;95(6):729-31.   DOI
23 Kumar V, et al. Robbins basic pathology 8th rev. ed. Seoul:Epublic. 2009:31-5, 57-8.
24 Male D, Brostoff J, Gray A. Immunology. London:Mosby. 1998:61-9, 229-32.
25 The society of Korean medicine obstetrics and gynecology. Korean medicine obstetrics and gynecology. 3rd rev. ed. Seoul: Euiseongdang. 2016:164, 171, 331-42.
26 Kyunghee university Korean medicine hospital. Hanbangjejehaeseoljip(韓方製劑解說集(全)). Seoul:Kyunghee university medicine center. 1983:134.
27 Wee HS, et al. Pregnancy of infertility with endometriosis after oriental medicine treatment diagnosed as a IVF case. A case report. The Journal Of Oriental Obstetrics & Gynecology. 2010;23(3):213-20.
28 Cho SH, Jin CS. The clinical study of PID patient by oriental medical treatment(focused on herbal medication & retention enema). The Journal of Oriental Obstetrics & Gynecology. 2002;15(3):181-9.
29 Song YR, et al. The clinical study one pelvic inflammatory disease(PID) patient treatments by Korean medicine. The Journal of Oriental Obstetrics & Gynecology.2014;27(3):158-67.   DOI
30 Wu C, et al. Neocryptotanshinone inhibits lipopolysaccharide-induced inflammation in RAW264.7 macrophages by suppression of NF-κB and iNOS signaling pathways. Acta Pharmaceutica Sinica B. 2015;5(4):323-9.   DOI
31 Kim DH, et al. Rubi Fructus Water Extract Alleviates LPS-Stimulated Macrophage Activation via an ER Stress-Induced Calcium/CHOP Signaling Pathway. Nutrients. 2020;12(11):3577.   DOI
32 Kim YH, et al. Biological Properties of Vibrio vulnificus Lipopolysaccharide and compared to those of Escherichia coli and Salmonella typhimurium Lipopolysaccharides. Korean journal of microbiology. 1989;27(2):147-54.
33 John MAS, et al. Interleukin 6 and interleukin 8 as potential biomarkers for oral cavity and oropharyngeal squamous cell carcinoma. Archives of Otolaryngology-Head & Neck Surgery. 2004;130(8):929-35.   DOI
34 Abul K, et al. Cellular and molecular immunology 7th rev. ed. Seoul: Panmuneducation. 2013:84-9, 180-6.
35 Tsevat DG, et al. Sexually transmitted diseases and infertility. American journal of obstetrics and gynecology. 2017;216(1):1-9.   DOI
36 Lee KS, Song BK. Clinical observation on Cheongpochookeo-tang. The Journal Of Oriental Obstetrics & Gynecology. 1987;1(1):33-7.
37 Ban HR, Lee JA, Cho SH. A case of chronic pelvic inflammatory disease. The Journal of Oriental Obstetrics & Gynecology. 2005;18(1):253-61.
38 Chung WS. The anti-inflammatory effects of Achyranthes japonica. Department of Medicine, Graduate school of Wonkwang university. 2011.
39 Patel S, et al. RIP1 inhibition blocks inflammatory diseases but not tumor growth or metastases. Cell Death & Differentiation, 2020;27(1):161-75.   DOI
40 Ren K, Torres R. Role of interleukin-1β during pain and inflammation. Brain research reviews. 2009;60(1):57-64.   DOI
41 Tateda K, et al. Lipopolysaccharide-induced lethality and cytokine production in aged mice. Infect Immun. 1996;64(3):769-74.   DOI
42 Ryu HK, et al. Research of the Anti-inflammatory Effects of Forsythiae Fructus and Lonicerae Flos Ethanol Extracts. The Journal of Korean Obstetrics and Gynecology. 2020;33(3):40-59.   DOI
43 Lee AK, et al. Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-alpha and COX-2 expression by sauchinone effects on I-kappa B alpha phosphorylation, C/EBP and AP-1 activation. Br J Pharmacol. 2003;139(1):11-2.   DOI
44 Korean Society of Obstetrics and Gynecology. Gynecology. 5th rev. ed. Seoul:Korea Medical Book Publishing Company. 2015:154, 546.
45 Boveris A, Alvarez S, Navarro A. The role of mitochondrial nitric oxide synthase in inflammation and septic shock. Free radical biology and medicine. 2002;33(9):1186-93.   DOI
46 Kishimoto T, et al. Interleukin-6 family of cytokines and gp130. Blood. 1995;86(4):1243-54.   DOI
47 Ramseyer VD, Garvin JL. Tumor necrosis factor-α: regulation of renal function and blood pressure. American Journal of Physiology-Renal Physiology. 2013;304(10):1231-42.
48 Turner PV, et al. Administration of substances to laboratory animals: routes of administration and factors to consider. Journal of the American Association for Laboratory Animal Science. 2011;50(5):600-13.
49 Jones SA, Brendan JJ. Recent insights into targeting the IL-6 cytokine family in inflammatory diseases and cancer. Nature Reviews Immunology. 2018;18(12):773-89.   DOI
50 Kim M, et al. The anti-inflammatory effect of Achyranthes japonica on lipopolysaccharide-induced inflammatory activity in murine macrophages. The Korea Journal of Herbology. 2011;26(2):51-7.   DOI
51 Grodstein F, Goldman MB, Cramer DW. Relation of tubal infertility to history of sexually transmitted diseases. American journal of epidemiology. 1993;137(5):577-84.   DOI
52 Sharma JN, Al-Omran A, Parvathy S.S. Role of nitric oxide in inflammatory diseases. Inflammopharmacology. 2007;15(6):252-9.   DOI
53 Tewari D, et al. Role of Nitric oxide in neurodegeneration: Function, regulation and inhibition. Curr Neuropharmacol. 2020;18(1):1-13.   DOI
54 Yun HJ, et al. Inhibitory effect of Coicis semen extract(CSE) on pro-inflammatory mediatory. The Journal of Pediatrics of Korean Medicine. 2009;23(1):159-71.