Browse > Article
http://dx.doi.org/10.15204/jkobgy.2021.34.3.015

The Effects of Liriopis Tuber Water Extract on Innate Immune Activation and Anti-Inflammation  

Kang, Nu-Ri (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
Hwang, Deok-Sang (Dept. of Korean Gynecology, College of Korean Medicine, Kyung Hee University)
Lee, Jin-Moo (Dept. of Korean Gynecology, College of Korean Medicine, Kyung Hee University)
Lee, Chang-Hoon (Dept. of Korean Gynecology, College of Korean Medicine, Kyung Hee University)
Jang, Jun-Bock (Dept. of Korean Gynecology, College of Korean Medicine, Kyung Hee University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.34, no.3, 2021 , pp. 15-28 More about this Journal
Abstract
Objectives: This study was designed to examine the anti-cancer activity by innate immunomodulating and anti-inflammatory effects of liriopis tuber water extract (LPE). Methods: Cell cytotoxicity was tested with 4T1 mouse mammary carcinoma cells, spleen cells, macrophage, and RAW264.7 cells. To investigate innate immunomodulating effects of LPE on macrophage, we measured tumor necrosis factor-alpha (TNF-α), interleukin-12 (IL-12), and interleukin-10 (IL-10). To investigate innate immunomodulating effects of LPE on RAW264.7 cell, we measured TNF-α, interleukin-6 (IL-6). In addition, TNF-α and nitric oxide (NO) induced by lipopolysaccharide (LPS) were measured after treating with LPE to observe innate immunomodulating effect of LPE on RAW264.7 cell. Also, mitogen-activated protein kinase (MAPK) and nuclear factor κB (NF-κB) were examined by western blot analysis. Results: In an in vitro cytotoxicity analysis, LPE affected tumor cell growth above specific concentration. As compared with the control group, the production of TNF-α, IL-12, and IL-10 were increased in macrophage. As compared with the control group, the production of TNF-α and IL-6 were increased in RAW 264.7 cell. The expression of TNF-α and NO induced by LPS after treating LPE was decreased. In addition, treatment of RAW 264.7 cell with LPE increased the phosphorylation levels of p-extracellular signal-regulated kinase (p-ERK), p-Jun N-terminal kinase (p-JNK), and p-p38. Conclusions: LPE might have impact on the anti-cancer effect by activation of innate immune system and inflammation control.
Keywords
Liriopis Tuber Water Extract; Cancer; Innate Immune System; Anti-Inflammation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Renping Z, et al. DT-13, a Saponin of Dwarf Lilyturf Tuber, Exhibits Anti-Cancer Activity by Down-Regulating C-C Chemokine Receptor Type 5 and Vascular Endothelial Growth Factor in MDA-MB-435 Cells. Chin J Nat Med. 2014;12(1):24-9.   DOI
2 Guha M, Mackman N. LPS Induction of Gene Expression in Human Monocytes. Cell Signal. 2001;13(2):85-94.   DOI
3 Lee AK, et al. Inhibition of Lipopolysaccharide-Inducible Nitric Oxide Synthase, TNF-α and COX-2 Expression by Sauchinone Effects on I-κBα Phosphorylation, C/EBP and AP-1 Activation. British journal of pharmacology. 2003;139(1):11-20.   DOI
4 Lee SK, Park JH, Kim YT. A Study on the Antioxidation and Antimicrobial Effect of "Megmoondong(Liriope platyphylla Wang et Tang)" Water Extracts. Korean J Food & Nutr. 2009;22(2):279-85.
5 Roh SS, et al. Studies of Anti-inflammation of Liriopis Tuber to Autoimmunune Diabetes in NOD Mice. Korean J. Oriental Physiology & Pathology. 2008;22(4):766-70.
6 An JY. The Effect of Liriopis Tuber on Liver Function Activity. Department of Pharmacy, Graduate School, Taegu-Hyosung Catholic University. 2000.
7 Song YC, et al. A Potential Herbal Adjuvant Combined With a Peptide-Based Vaccine Acts Against HPV-Related Tumors Through Enhancing Effector and Memory T-Cell Immune Responses. Frontiers in immunology. 2020;11(62):1-15.   DOI
8 Oriental Gynecology Textbook Compilation Committee. Korean Medicine Obstetric & Gynecology I. Seoul:Jeongdam. 2007:282-310.
9 Kim JH, et al. Review on Clinical Study of Oriental Medical Treatment for Cancer in Korea. J Acupunct Res. 2012;29(3):19-28.
10 Bae SK, et al. Modulatory Effects of Cytokine Release in Immune Response of RAW 264.7 and TK-1. J Int Korean Med. 2018;39(6):1244-55.   DOI
11 Heo J. Dongeuibogam. Gyeongnam: Dongeuibogam Publishing Co. 2004:2149.
12 National College of Korean Medicine Textbook Compilation Committee. Herbology. Seoul:Youglimsa. 2008:644-5.
13 Hongzhi D, et al. DT-13 Inhibits Cancer Cell Migration by Regulating NMIIA Indirectly in the Tumor Microenvironment. Oncol Rep. 2016;36(2):721-8.   DOI
14 Im JG, et al. Dietary Effect of Liriopis Tuber Water Extracts on the Level of Blood Glucose and Serum Cholesterol in Streptozotocin-Induced Diabetic Rat. J East Asian Soc Dietary Life. 2005; 15(1):20-8.
15 Baek NI, et al. Cytotoxicity of Steroid-Saponins from the Tuber of Liriope Platyphylla W.T. Agricultural Chemistry and Biotechnology. 1998;41(5):390-4.
16 Li H, et al. DT-13, a Saponin Monomer of Dwarf Lilyturf Tuber, Induces Autophagy and Potentiates Anti-Cancer Effect of Nutrient Deprivation. Eur J Pharmacol. 2016;781:164-72.   DOI
17 Li S, et al. The Saponin Monomer of Dwarf Lilyturf Tuber, DT-13, Reduces Human Breast Cancer Cell Adhesion and Migration during Hypoxia via Regulation of Tissue Factor. Biol Pharm Bull. 2010;33(7):1192-8.   DOI
18 Renping Z, et al. The Saponin Monomer of Dwarf Lilyturf Tuber, DT-13, Inhibits Angiogenesis Under Hypoxia and Normoxia via Multi-Targeting Activity. Oncol Rep. 2013;29(4):1379-86.   DOI
19 Yamamoto, et al. Soy, Isoflavones, and Breast Cancer Risk in Japan. J of the National Cancer Institute. 2003;95(12):906-13.   DOI
20 Suh DS, et al. Apoptotic Effect of NV-196, an Isoflavone Derivative, in Epithelial Ovarian Cancer Cells. Korean J Obstet Gynecol. 2011;54(2):86-92.   DOI
21 Peroval MY, et al. A Critical Role for MAPK Signaling Pathways in the Transcriptional Regulation of Toll Like Receptors. PLoS One. 2013;8(2):e51243.   DOI
22 Bauman JE, Ferris RL. Integrating Novel Therapeutic Monoclonal Antibodies into the Management of Head and Neck Cancer. Cancer. 2014;120(5):624-32.   DOI
23 Kang YG, Lee TH. Liriopis Tuber Improves Stress-Induced Memorial Impairments in Rats. Kor J Herbology. 2006;21(2):63-75.
24 Aderem A, Ulevitch RJ. Toll-like Receptors in the Induction of the Innate Immune Response. Nature. 2000;406(6797):782-7.   DOI
25 Cho JK. Oriental Clinical Oncology. 1st ed. Daejeon:Jumin Publishing Co. 2001:9-15.
26 Kim DH, et al. Hwangjenaegyeong somunhaeseog-Ha. 1st ed. Seoul:Uiseongdang. 2001:746.
27 Chang WS. Hwalbeobgiyo. Seoul:Daeseong Publishing Co. 1986:129-30.
28 Kim IG, et al. Mixture Bombyx mori L. and Liriopis seu Ophiopogonis Tuber effects on T cells in Thymus, Lymph Nodes. Kor J Herbol. 2018;33(5):47-52.   DOI
29 Rosadini CV, Kagan JC. Early Innate Immune Responses to Bacterial LPS. Curr Opin Immunol. 2017;44:14-9.   DOI
30 Fukao T, Koyasu S. PI3K and Negative Regulation of TLR Signaling. Trends Immunol. 2003;24(7):358-63.   DOI
31 Schorey JS, Cooper AM. Macrophage Signaling upon Mycobacterial Infection: the MAP Kinases Lead the Way. Cell Microbiol. 2003;5(3):133-42.   DOI
32 Gaestel M, et al. Protein Kinases as Small Molecule Inhibitor Targets in Inflammation. Curr Med Chem. 2007;14(21):2214-34.   DOI
33 Medzhitov R, Preston-Hurlburt P, Janeway CA. A Human Homologue of the Drosophila Toll Protein Signals Activation of Adaptive Immunity. Nature. 1997;388(6640):394-7.   DOI
34 Kurebayashi J, et al. Medroxyprogesterone Acetate Inhibits Interleukin 6 Secretion from KPL-4 Human Breast Cancer Cells Both in vitro and in vivo: a Possible Mechanism of the Anticachectic Effect. Br J Cancer. 1999;79(3/4):631-6.   DOI
35 Jiang MH, Zhu L, Jiang JG. Immunoregulatory actions of polysaccharides from Chinese herbal medicine. Expert Opin Ther Targets. 2010;14(12):1367-402.   DOI
36 Ha ES, et al. Anti-metastatic Activity of Glycoprotein Fractionated from Acanthopanax Senticosus, Involvement of NK-cell and Macrophage Activation. Arch Pharm Res. 2004;27(2):217-24.   DOI
37 Abbas L, et al. Cellular and Molecular Immunology. 5th ed. Netherlands:Elsevier Health Sciences. 2003:247-63.
38 Gee K, et al. Differential Regulation of CD44 Expression by Lipopolysaccharide (LPS) and TNF-alpha in Human Monocytic Cells: Distinct Involvement of C-Jun N-terminal Kinase in LPS-Induced CD44 Expression. J Immunol. 2002;169(10):5660-72.   DOI
39 Takeda K, Kaisho T, Akira S. Toll-Like Receptors. Annu Rev Immunol. 2003;21:335-76.   DOI
40 Paik YH, et al. Toll-Like Receptor 4 Mediates Inflammatory Signaling by Bacterial Lipopolysaccharide in Human Hepatic Stellate Cells. Hepatology. 2003;37(5):979-82.   DOI
41 Adlercreutz H. Phytoestrogens: Epidemiology and a Possible Role in Cancer Protection. Environmental Health Perspectives. 1995;103 Suppl 7(Suppl 7):103-12.
42 Delgado AV, McManus AT, Chambers JP. Production of Tumor Necrosis Factor-alpha, Interleukin 1-beta, Interleukin 2, and Interleukin 6 by Rat Leukocyte Subpopulations after Exposure to Substance P. Neuropeptides. 2003;37(6):355-61.   DOI
43 William EP. Fundamental Immunology. 5th ed. USA:Lippincott Williams & Wilkins. 2003:20.