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http://dx.doi.org/10.15204/jkobgy.2021.34.2.001

Innate Immunity Activation and Anti-Inflammation Effects of Evodia Rutaecarpine Water Extract  

Jeong, So-Mi (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
Lee, Jin-Moo (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
Lee, Chang-Hoon (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
Hwang, Deok-Sang (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
Jang, Jun-Bock (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.34, no.2, 2021 , pp. 1-15 More about this Journal
Abstract
Objectives: This study was designed to examine immuno-modulatory effects of Evodia Rutaecarpine by activating innate immune system and inhibiting inflammation. Methods: First, Cell cytotoxicity was examined with 4T1 breast carcinoma and TG-induced macrophage. To investigate activating innate immune system of Evodiamine Rutacarpine Extract (ERE) on macrophage, we tested tumor necrosis factor-alpha (TNF-α), interleukin-12 (IL-12), and interleukin-6 (IL-6). In addition, TNF-α and nitric oxide (NO) induced by lipopolysaccharide (LPS) were measured after treating with ERE to observe innate immune modulating effect of ERE on RAW 264.7 cell. Also, mitogen-activated protein kinase (MAPK) and nuclear factor κB (NF-κB) were examined by western blot analysis. Results: In cytotoxicity analysis, ERE significantly affected tumor cell growth above specific concentration. Also, ERE significantly affected macrophage growth above specific concetration. As compared with the control group, the production of TNF-α, IL-12 and IL-6 were increased in TG-induced macrophage. As compared with the control group, TNF-α and IL-6 were significantly up-regulated in RAW 264.7 cell. The expression of TNF-α and NO induced by LPS after treating ERE was significantly decreased compared with control group. In addition, We observed ERE inhibited the phosphorylation levels of p-extracellular signal-regulated kinase (p-ERK), p-Jun N-terminal kinase (p-JNK), and p-p38 in western blotting by treating ERE on RAW 264.7 cell. Conclusions: ERE seems to have considerable impact on the anti-cancer effect by activation of innate immune system and inflammation control.
Keywords
Evodia Rutaecarpine; Anti-Inflammation; Innate Immunity; Cancer; Immune-Modulatory;
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