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http://dx.doi.org/10.15204/jkobgy.2021.34.1.034

Anti-inflammatory Effect and Mechanism of Citri Reticulatae Viride Pericarpium Water Extract  

Ahn, Tae-Seok (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
Hwang, Deok-Sang (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
Lee, Jin-Moo (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
Jang, Jun-Bock (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
Lee, Chang-Hoon (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.34, no.1, 2021 , pp. 34-47 More about this Journal
Abstract
Objectives: This study was designed to examine anti-inflammatory effect and mechanism of Citri Reticulatae Viride Pericarpium water extract (CRE). Methods: Cell cytotoxicity was tested with RAW 264.7 cells. To investigate anti-inflammatory effect of CRE in lipopolysaccharide (LPS)-induced RAW 264.7 cell, we measured nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10). In addition, mitrogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) were examined by western blotting in LPS-induced RAW 264.7 cell with treated CRE. Results: In cytotoxicity analysis, CRE does not affect cell cytotoxicity. As compared with the control group, the expression of NO, PGE2, TNF-α, IL-6 were significantly decreased, and IL-10 was significantly increased in LPS-induced RAW 264.7 cell with treated CRE. As a result of Western blotting, there was concentration-dependent inhibition of pp38, pERK in MAPK pathway and significant reduction of pp65 in the NF-κB pathway. Conclusions: CRE might have anti-inflammatory effect in LPS-induced macrophages by promoting the production of IL-10.
Keywords
Citri Reticulatae Viride Pericarpium; Anti-inflammatory; Lipopolysaccharide; Macrophage; Interleukin-10;
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