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http://dx.doi.org/10.15204/jkobgy.2019.32.3.001

Selection of the Fittest Anti-osteoporotic Mixed Compositions Consist of Morindae Radix and Cistanchis Herba Aqueous Extracts on Ovariectomized Mice  

An, Tteul-E-Bom (Dept. of Korean Obstetrics & Gynecology, College of Korean Medicine, Daegu Haany University)
Kim, Dong-Chul (Dept. of Korean Obstetrics & Gynecology, College of Korean Medicine, Daegu Haany University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.32, no.3, 2019 , pp. 1-19 More about this Journal
Abstract
Objectives: To select the optimal ranges showing obvious synergic anti-osteoporotic potential after adjust mixed formula consisted of Morindae Radix (MR) and Cistanchis Herba (CH) as compared with those of each single formula or risedronate sodium (RES) using bilateral ovariectomized (OVX) female mice. Methods: Fourteen groups, total eight sham or 104 OVX mice were selected based on the body weights at 34 days after OVX surgery. After that, 9 types mixed compositions, single formula of MR and CH, and RES were orally administered for 35 days. And we measured changes in body weight and gain, femur weight, bone mineral density (BMD), bone strength (failure load) and mineral content - calcium (Ca) and inorganic phosphorus (IP), osteocalcin contents and bone specific alkaline phosphatase (bALP) activities of all mice. Results: The OVX-induced estrogen-deficient osteoporotic signs were significantly inhibited by 35 days of continuous oral treatment of all treated mice as compared with OVX control mice. Especially, MR:CH 1:3 and 1:1 mixed formula treated mice showed significantly more favorable inhibitory activities against estrogen-deficient osteoporosis symptoms as compared to those of each single formula of MR and CH. Although RES also ameliorated the decreases of the femur BMD, strength and trabecular bone architectures through the inhibited the increases of bone turnover, but they did not critically influenced on the bone formations. Conclusions: The results suggest that MR:CH 1:3 mixed formula showed somewhat lower anti-resorptive effects as compared to those of RES, but they also showed bone formation effects. therefore, it is expected that MR:CH 1:3 mixture will be promising as a potent protective agents for relieving the osteoporosis in menopausal women.
Keywords
Morindae Radix; Cistanchis Herba; OVX; Menopause; Osteoporosis;
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1 Sambrook P, Cooper C. Osteoporosis. Lancet. 2006;367(9527):2010-8.   DOI
2 Korea Centers for Disease Control and Prevention. 2010 National Health Statistics and 2010 Health Behavior and Chronic Disease Statistics[cited October 18, 2018]. Available from:URL: https://knhanes.cdc.go.kr/knhanes/sub04/sub04_03.do.
3 National Osteoporosis Foundation. Clinician's guide prevention and treatment of osteoporosis. 2008.
4 International Osteoporosis Foundation. Who's at risk?[cited October 18, 2018]. Available from:URL:https://www.iofbonehealth.org/whos-risk.
5 The Society of Korean Medicine Obstetrics and Gynecology. Oriental Obstetrics & Gynecology 3rd edition. Seoul:Euiseongdang publisher. 2016:252-4.
6 World Health Organization. Prevention and Management of Osteoporosis. 2003.
7 Korean Society for Bone and Mineral Research. Physician's guide for diagnosis & treatment of osteoporosis. 2015.
8 Camacho PM, et al. American Association of Clinical Endocrinologists and American College of Endocrinology Clinical Practice guidelines for the diagnosis and treatment of postmenopausal osteoporosis - 2016. Endocr Pract. 2016;22(4):1-42.   DOI
9 Gennari L, et al. Treatment needs and current options for postmenopausal osteoporosis. Expert Opin Pharmacother. 2016;17(8):1141-52.   DOI
10 Seo MS, et al. The Review on the Study of Osteoporosis in Korean Medicine Journals. The Journal of Korea CHUNA Manual Medicine for Spine & Nerves. 2013;8(2):67-78.
11 Kim JY, Song YS. The Study about the Comparison of Korean-Western Medicine on Osteoporosis. Journal of Oriental Rehabilitation Medicine. 1996;6:293-315.
12 Kim HG, Yu JY, Lee EJ. The study about the comparison of Korean- Western medicine on sin-ju-gol(腎主骨). The Journal of Korean Oriental Internal Medicine. 1991;12(2):26-9.
13 Lee CH, Lee IS. The Study of Document on Osteoporosis through Oriental Medicine. Dong-Eui Institute of Oriental Medicine. 2000 Dec;4:107-27.
14 National College of Oriental Medicine Common Textbook Compilation Committee. Herbal medicine(本草學). Seoul:Yeonglimsa. 2008:594-7.
15 Zhang H, et al. Antioxidant activity and physicochemical properties of an acidic polysaccharide from Morinda officinalis. Int J Biol Macromol. 2013;58:7-12.   DOI
16 Wat E, et al. The protective effect of Herba Cistanches on statin-induced myotoxicity in vitro. J Ethnopharmacol. 2016;190:68-73.   DOI
17 Heo J. Dongeuibogam. Seoul:Bubinbooks. 2008.
18 Nam SH, et al. Topically administered Risedronate shows powerful antiosteoporosis effect in ovariectomized mouse model. Bone. 2012;50(1):149-55.   DOI
19 Kang SJ, et al. Selection of the Optimal Herbal Compositions of Red Clover and Pomegranate according to their Protective Effect against Climacteric Symptoms in Ovariectomized Mice. Nutrients. 2016;8(8):447.   DOI
20 Harris ST, et al. Effects of risedronate treatment on vertebral and nonvertebral fractures in women with postmenopausal osteoporosis: a randomized controlled trial. Vertebral Efficacy With Risedronate Therapy (VERT) Study Group. JAMA. 1999;282(14):1344-52.   DOI
21 Kang SJ, et al. Fermentation with Aquilariae Lignum enhances the antidiabetic activity of green tea in type II diabetic db/db mouse. Nutrients. 2014;6(9):3536-71.   DOI
22 Joo JH, et al. A Novel Pyrazole Derivative Protects from Ovariectomyinduced Osteoporosis through the Inhibition of NADPH oxidase. Sci Rep. 2016;6:22389.   DOI
23 Ludbrook J. Update: Microcomputer Statistics Packages. A personal view. Clin Exp Pharmacol Physiol. 1997;24(3-4):294-6.   DOI
24 Levene A. Pathological factors influencing excision of tumours in the head and neck. Part I. Clin Otolaryngol Allied Sci. 1981;6(2):145-51.   DOI
25 Choi JS, et al. Blood glycemiamodulating effects of melanian snail protein hydrolysates in mice with type II diabetes. Int J Mol Med. 2017;39(6):1437-51.   DOI
26 Manolagas SC, Kousteni S, Jilka RL. Sex steroids and bone. Recent Prog Horm Res. 2002;57:385-409.   DOI
27 Shin HD, et al. Antiosteoporotic effect of Polycan, beta-glucan from Aureobasidium, in ovariectomized osteoporotic mice. Nutrition. 2007;23(11-12):853-60.   DOI
28 Rodan GA, Martin TJ. Therapeutic Approaches to Bone Diseases. Science. 2000;289(5484):1508-14.   DOI
29 MacDonald BR, Gowen M. Emerging Therapies in Osteoporosis. Best Pract Res Clin Rheumatol. 2001;15(3):483-96.   DOI
30 Wu YB, et al. Antiosteoporotic Activity of Athraquinones from Morinda officinalis on Osteoblasts and Osteoclasts. Molecules. 2009;14(1):573-83.   DOI
31 Li TM, et al. Cistanche deserticola extract increases bone formation in osteoblasts. J Pharm Pharmacol. 2012;64(6):897-907.   DOI
32 Xu X, et al. Therapeutic Effect of Cistanoside A on bone metabolism of ovariectomized mice. Molecules. 2017;22(2):197.   DOI
33 Lorden JF, Caudle A. Behavioral and Endocrinological Effects of Single Injections of Monosodium Glutamate in the Mouse. Neurobehav Toxicol Teratol. 1986;8(5):509-19.
34 Kang SJ, et al. Dried pomegranate potentiates anti-osteoporotic and antiobesity activities of red clover dry extracts in ovariectomized rats. Nutrients. 2015;7(4):2622-47.   DOI
35 Choi JS, et al. Effect of Polycalcium, a mixture of Polycan and calcium lactate-gluconate in a 1:9 weight ratio, on rats with surgery-induced osteoarthritis. Exp Ther Med. 2015;9(5):1780-90.   DOI
36 Park SI, et al. The Effects of Topical Application of Polycal (a 2:98 (g/g) Mixture of Polycan and Calcium Gluconate) on Experimental Periodontitis and Alveolar Bone loss in Rats. Molecules. 2016;21(4):527.   DOI
37 Ke HZ, et al. Long-term treatment of lasofoxifene preserves bone mass and bone strength and dose not adversely affect the uterus in ovariectomized rats. Endocrinology. 2004;145(4):1996-2005.   DOI
38 Bain SD, et al. High-dose Estrogen Inhibits Bone Resorption and Stimulates Bone Formation in the Ovariectomized Mouse. J Bone Miner Res. 1993;8(4):435-42.   DOI
39 Andersson N et al. Repeated in vivo Determinations of Bone Mineral Density During Parathyroid Hormone Treatment in Ovariectomized Mice. J Endocrinol. 2001;170(3):529-37.   DOI
40 Yamamoto M, et al. The Integrin Ligand Echistatin Prevents Bone Loss in Ovariectomized Mice and Rats. Endocrinology. 1998;139(3):1411-9.   DOI
41 Ederveen AG, Kloosterboer HJ. Tibolone, a steroid with a tissue-specific hormonal profile, completely prevents ovariectomyinduced bone loss in sexually mature rats. J Bone Miner Res. 1999;14(11):1963-70.   DOI
42 Ismail F, et al. Serum bone gla protein and the vitamin D endocrine system in the oophorectomized rat. Endocrinology. 1988;122(2):624-30.   DOI
43 Park JH. Euiyuksinnongbonchokyung (意譯神農本草經). Seoul:Sinil seojeok. 2014:4-5.
44 Tanaka S, et al. Acute effects of ovariectomy on wound healing of alveolar bone after maxillary molar extraction in aged rats. Anat Rec. 2001;262(2):203-12.   DOI
45 Tarvainen R, et al. Clodronate Prevents Immobilization Osteopenia in Rats. Acta Orthop Scand. 1994;65(16):643-6.   DOI
46 Syed Z, Khan A. Bone Densitometry: applications and limitations. J Obstet Gynecol Can. 2002;24(6):476-84.   DOI
47 Diez F. Guidelines for the Diagnosis of Osteoporosis by Densitometric Methods. J Manipulative Physiol Ther. 2002;25(6):403-15.   DOI