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http://dx.doi.org/10.15204/jkobgy.2019.32.2.001

Anti-inflammatory Effects of Salvia Miltiorrhizae Radix Water Extract in RAW 264.7 Cells and Mouse Induced by Lipopolysaccharide  

Kim, Gun-Hee (Dept. of Korean Gynecology and Obstetrics, College of Korean Medicine, Wonkwang University)
Hong, Ka-Kyung (Dept. of Korean Gynecology and Obstetrics, College of Korean Medicine, Wonkwang University)
Cho, Han-Baek (Dept. of Korean Gynecology and Obstetrics, College of Korean Medicine, Wonkwang University)
Choi, Chang-Min (Dept. of Korean Gynecology and Obstetrics, College of Korean Medicine, Wonkwang University)
Kim, Song-Baek (Dept. of Korean Gynecology and Obstetrics, College of Korean Medicine, Wonkwang University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.32, no.2, 2019 , pp. 1-17 More about this Journal
Abstract
Objectives: This study was performed to identify the anti-inflammatory effects of Salvia miltiorrhizae radix Water extract (SMW) on lipopolysaccharide (LPS) induced inflammation. Methods: RAW 264.7 cells were treated with 500 ng/ml of LPS. SMW (0.1, 0.25, 0.5 mg/ml) was treated 1 h prior to LPS. Cell viability was measured by MTT assay. Levels of nitric oxide (NO) were measured with Griess reagent and pro-inflammatory cytokines were measured by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (PCR). We also examined molecular mechanisms such as mitogen-activated protein kinases (MAPKs) and nuclear factor-kappa B ($NF-{\kappa}B$) activation by western blot. In addition, we observed mice survival rate after LPS and examined their cytokine levels of serum and liver tissue. Results: SMW itself did not have cytotoxic effects in RAW 264.7 cells less than 0.5 mg/ml. SMW treatment inhibited the production of NO, and interleukin $(IL)-1{\beta}$ which is pro-inflammatory cytokine. And SMW treatment inhibited the LPS-induced activation of MAPKs such as extracellular signal-regulated kinase1/2 (ERK1/2), p38 kinases (p38), c-Jun NH2-terminal kinase (JNK) and $NF-{\kappa}B$. In addition, it also showed reducing the level of $IL-1{\beta}$ on the serum and liver tissue of mice. Also, death of LPS-induced mice was inhibited by SMW. Conclusions: The result suggests that treatment of SMW could reduce the LPS-induced inflammation. Thereby, SMW could be used as a protective agent against inflammation. Also, this study could give a clinical basis that SMW could be a drug or agent to prevent inflammatory diseases.
Keywords
Salvia miltiorrhiza radix; RAW 264.7 Cell; Lipopolysaccharide (LPS); Cytokine; Inflammation;
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Times Cited By KSCI : 6  (Citation Analysis)
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