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http://dx.doi.org/10.15204/jkobgy.2018.31.4.016

Anti-osteoporotic Activity of Gojineumja Aqueous Extracts on the Ovariectomized Mice  

Cho, Su-Yun (Dept. of Korean Obstetrics & Gynecology, College of Korean Medicine, Daegu Haany University)
Kim, Dong-Chul (Dept. of Korean Obstetrics & Gynecology, College of Korean Medicine, Daegu Haany University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.31, no.4, 2018 , pp. 16-38 More about this Journal
Abstract
Objectives: The objective of this in vivo study is to observe the anti-osteoporotic activities of Gojineumja aqueous extracts (GJEJ) on the ovariectomized (OVX) mice as compared to those of risedronate sodium (RES). Methods: Thirty five days after bilateral OVX, GJEJ was orally administered, for 35 days once a day and then the changes on the body weight and gain during experimental periods, femur weights, bone mineral density (BMD), bone strength (failure load), mineral contents - calcium (Ca) and inorganic phosphorus (IP), histological profiles and histomorphometrical analyses at sacrifice were conducted with serum biochemistry - osteocalcin contents and bone specific alkaline phosphatase (BALP) activities. And the results of GJEJ were compared with RES orally administered OVX mice. Results: As a result of OVX, noticeable increase of body weight and gains and serum osteocalcin levels, decrease of serum BALP activities, femur weights, femur Ca and IP contents, BMD and strength were observed as compared to those of sham control mice, respectively. Also, the decrease of all histomorphometrical indices indicating the bone mass and structure, and the increase of indices about resorption were also detected in the femur of OVX control. However, these estrogen-deficient osteoporotic signs were significantly and dose-dependently inhibited by 35 days of continuous oral treatment of GJEJ, at dose levels of 500, 250 and 125 mg/kg, respectively. Especially, GJEJ 500 mg/kg showed favorable inhibitory activities against estrogen-deficient osteoporosis symptoms induced by OVX as comparable to those of RES 2.5 mg/kg. Conclusions: The results in this study suggest that oral administrations of GJEJ have clear dose-dependent favorable anti-osteoporotic activities in OVX mice.
Keywords
Osteoporosis; Gojineumja; Ovariectomy (OVX);
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1 Editing commission of herbal medicine. Bon-Cho-Hak(本草學). 2nd rev. ed. Seoul:Yeong-Rim-Sa. 2011:581, 603-4, 607-8, 633-4, 681, 688.
2 Cho CY, et al. Effects of Bia-hwan (Feier-wan) on the Ovariectomized Rat Model of Osteoporosis. Journal of Korean Medicine Rehabilitation. 2017;27(2):19-27.   DOI
3 Kim JH, et al. Effects of Cuscutae Semen Extract on Prevention of Osteoporosis in Ovariectomized Rats. J Korean Obstet Gynecol. 2012;25(4):1-11.   DOI
4 Boonen S, et al. Preventing osteoporotic fractures with antiresorptive therapy: implications of microarchitectural changes. J Intern Med. 2004;255(1):1-12.   DOI
5 Bae DC, Stein BS. The diagnosis and treatment of osteoporosis in men on androgen deprivation therapy for advanced carcinoma of the prostate. J Urol. 2004;172(6 Pt 1):2137-44.   DOI
6 Roux C, et al. Efficacy of risedronate on clinical vertebral fractures within six months. Curr Med Res Opin. 2004;20(4):433-9.   DOI
7 Ito M, et al. Effects of risedronate on trabecular microstructure and biomechanical properties in ovariectomized rat tibia. Osteoporos Int. 2005;16(9):1042-8.   DOI
8 Lorden JF, Caudle A. Behavioral and endocrinological effects of single injections of monosodium glutamate in the mouse. Neurobehav Toxicol Teratol. 1986;8(5):509-19.
9 Bain SD, et al. High-dose estrogen inhibits bone resorption and stimulates bone formation in the ovariectomized mouse. J Bone Miner Res. 1993;8(4):435-42.   DOI
10 Sandstedt J, et al. Elevated levels of growth hormone increase bone mineral content in normal young mice, but not in ovariectomized mice. Endocrinology. 1996;137(8):3368-74.   DOI
11 Andersson N, et al. Repeated in vivo determinations of bone mineral density during parathyroid hormone treatment in ovariectomized mice. J Endocrinol. 2001;170(3):529-37.   DOI
12 Tarvainen R, et al. Clodronate prevents immobilization osteopenia in rats. Acta Orthop Scand. 1994;65(6):643-6.   DOI
13 Yamamoto M, et al. The integrin ligand echistatin prevents bone loss in ovariectomized mice and rats. Endocrinology. 1998;139(3):1411-9.   DOI
14 Ederveen AG, Kloosterboer HJ. Tibolone, a steroid with a tissue-specific hormonal profile, completely prevents ovariectomyinduced bone loss in sexually mature rats. J Bone Miner Res. 1999;14(11):1963-70.   DOI
15 Ke HZ, et al. Long-term treatment of lasofoxifene preserves bone mass and bone strength and dose not adversely affect the uterus in ovariectomized rats. Endocrinology. 2004;145(4):1996-2005.   DOI
16 Syed Z, Khan A. Bone densitometry: applications and limitations. J Obstet Gynaecol Can. 2002;24(6):476-84.   DOI
17 Diez F. Guidelines for the diagnosis of osteoporosis by densitometric methods. J Manipulative Physiol Ther. 2002;25(6):403-15.   DOI
18 Tanaka S, et al. Acute effects of ovariectomy on wound healing of alveolar bone after maxillary molar extraction in aged rats. Anat Rec. 2001;262(2):203-12.   DOI
19 Heikkinen T, Puolivali J, Tanila H. Effects of long-term ovariectomy and estrogen treatment on maze learning in aged mice. Exp Gerontol. 2004;39(9) :1277-83.   DOI
20 Jakubas-Przewlocka J, Przewlocki P, Sawicki A. Assessment of changes due to the long-term effect of estrogen and calcium deficiency in the trabecular bone structure in rats. Clin Exp Rheumatol. 2005;23(3):385-8.
21 Murakami H, et al. Effects of tiludronate on bone mass, structure, and turnover at the epiphyseal, primary, and secondary spongiosa in the proximal tibia of growing rats after sciatic neurectomy. J Bone Miner Res. 1994;9(9):1355-64.   DOI
22 Huh J. Dongeuibogam(東醫寶鑑). Seoul: Namsandang. 1966:447.
23 Korean Society of Obstetrics and Gynecology. Gynecology, 5th ed. Seoul:Korea Medical Publishing Company. 2015:659-61.
24 The Korean Association of Internal Medicine. Harrison's Internal Medicine, 17th ed. Seoul:MIP publication. 2010:2883-95.
25 Yamaguchi K, et al. Suppressive effect of norzoanthamine hydrochloride on experimental osteoporosis in ovariectomized mice. Biol Pharm Bull. 1999;22(9):920-4.   DOI
26 Weinreb M, et al. Short-term healing kinetics of cortical and cancellous bone osteopenia induced by unloading during the reloading period in young rats. Virchows Arch. 1997;431(6):449-52.   DOI
27 Kang SG, Park YB, Ahn HS. The Bibliographical Studies on the Acupuncture Treatment of the Osteoporosis. K.A.M.S. 1995;15(2):171-90.
28 Kim HJ, Lee TG. Literature Review of Menopausal osteoporosis. J Korean Obstet Gynecol. 1998;11(1):131-48.
29 Lee C. Introduction to Medicine(醫學入門). Seoul:Bubin Publisher. 2009:1892.
30 Kang DW, Kang SB. Effects of Gojineumja on immunosuppression induced by methotrexate in rat. Korean J Orient Int Med. 2004;25(4):117-28.
31 Kim HJ, Jung IC, Lee SR. Effect of Gojineumja(Guzhenyinzi) on neural tissue degeneration in mouse model of Alzheimer disease. J Oriental Neuropsychiatry. 2009;20(2):31-46.
32 Won CH, et al. Effect of Kojinyumja on galactosamine induced hepatotoxicity in rats. Dongguk J the Institute of Oriental Medicine. 1997;6(1):137-49.
33 Lim DW, Kim YT. Dried root of Rehmannia glutinosa prevents bone loss in ovariectomized rats. Molecules. 2013;18(5):5804-13.   DOI
34 Han N, et al. The in vivo effects of a fraction from Dioscorea spongiosa on glucocorticoid-induced osteoporosis. J Ethnopharmacol. 2016;185:53-9.   DOI
35 Shin HD, et al. Antiosteoporotic effect of Polycan, beta-glucan from Aureobasidium, in ovariectomized osteoporotic mice. Nutrition. 2007;23(11-12):853-60.   DOI
36 Joo JH, et al. A novel pyrazole derivative protects from ovariectomyinduced osteoporosis through the inhibition of NADPH oxidase. Sci Rep. 2016;6:22389.   DOI
37 Kang SJ, et al. Selection of the optimal herbal compositions of red clover and pomegranate according to their protective effect against climacteric symptoms in ovariectomized mice. Nutrients. 2016;8(8):447.   DOI
38 Kang SJ, et al. Fermentation with Aquilariae Lignum enhances the antidiabetic activity of green tea in type II diabetic db/db mouse. Nutrients. 2014;6(9):3536-71.   DOI
39 Harris ST, et al. Effects of risedronate treatment on vertebral and nonvertebral fractures in women with postmenopausal osteoporosis: a randomized controlled trial. Vertebral Efficacy With Risedronate Therapy (VERT) Study Group. JAMA. 1999;282(14):1344-52.   DOI
40 Nam SH, et al. Topically administered Risedronate shows powerful anti-osteoporosis effect in ovariectomized mouse model. Bone. 2012;50(1):149-55.   DOI
41 Choi JS, et al. Blood glycemia-modulating effects of melanian snail protein hydrolysates in mice with type II diabetes. Int J Mol Med. 2017;39(6):1437-51.   DOI
42 Manolagas SC, Kousteni S, Jilka RL. Sex steroids and bone. Recent Prog Horm Res. 2002;57:385-409.   DOI
43 Melton LJ 3rd, et al. How many women have osteoporosis? J Bone Miner Res. 2005;20(5):886-92.   DOI
44 Rodan GA, Martin TJ. Therapeutic approaches to bone diseases. Science. 2000;289(5484):1508-14.   DOI
45 Gowen M, Emery JG, Kumar S. Emerging therapies for osteoporosis. Emerging Drugs. 2000;5:1-43.   DOI
46 Devipriya N, et al. Effect of ellagic acid, a natural polyphenol, on alcoholinduced prooxidant and antioxidant imbalance: a drug dose dependent study. Singapore Med J. 2007;48(4):311-8.
47 Kim HS, et al. Single oral dose toxicity test of blue honeysuckle concentrate in mice. Toxicol Res. 2015;31(1):61-8.   DOI
48 Noh JR, et al. Hepatoprotective effect of Platycodon grandiflorum against chronic ethanolinduced oxidative stress in C57BL/6 mice. Ann Nutr Metab. 2011;58(3):224-31.   DOI