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http://dx.doi.org/10.15204/jkobgy.2017.30.4.001

Effects of Hyeolbuchugeo-tang on Osteoclast Differentiation and Bone Resorption  

Jang, Sae-Byul (Dept. of Oriental Gynecology, College of Korean Medicine, Dae-Jeon University Graduate School)
Yoo, Dong-Youl (Dept. of Oriental Gynecology, College of Korean Medicine, Dae-Jeon University Graduate School)
Yoo, Jeong-Eun (Dept. of Oriental Gynecology, College of Korean Medicine, Dae-Jeon University Graduate School)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.30, no.4, 2017 , pp. 1-17 More about this Journal
Abstract
Objectives: This study was conducted to evaluate the effects of Hyeolbuchugeo-tang (HBC) on Osteoporosis. Methods: We induced RAW 264.7 cells to differentiate to Osteoclasts by RANKL and treated RANKL-induced RAW 264.7 cells with HBC (0, 150, 350, $700{\mu}g/ml$). To measure osteoclast differentiation and activation, we counted TRAP (+) MNCs and measured mRNA expressions of its related genes (TRAP, MMP-9, cathepsin K, NFATc1, c-Fos, MITF, iNOS, COX-2, TNF-${\alpha}$) by RT-PCR. To assess bone resorption, the Bone pit formation were examined under a microscope. Results: HBC decreased TRAP (+) MNCs and inhibited mRNA expressions of TRAP, MMP-9, cathepsin K, NFATc1, c-Fos, MITF in osteoclast. And HBC inhibited Bone pit formation. Conclusions: HBC inhibited osteoclast differentiation and activation and bone resorption. Taken together, these results indicate that HBC might have potentials for prevention and treatment of Osteoporosis.
Keywords
Hyeolbuchugeo-tang; Osteoporosis; Osteoclasts; Osteoclastogenesis; Bone Resorption; Bone Pit;
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Times Cited By KSCI : 9  (Citation Analysis)
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