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http://dx.doi.org/10.15204/jkobgy.2015.28.2.026

Anti-cancer Effects of Dendropanax Morbifera Extract in MCF-7 and MDA-MB-231 Cells  

Im, Kyu-Jung (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University)
Jang, Sae-Byul (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University)
Yoo, Dong-Youl (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.28, no.2, 2015 , pp. 26-39 More about this Journal
Abstract
Objectives : Dendropanax morbifera is known as a tree that has been used in traditional medicine for various diseases. However, its biological activities in cancer have not yet been clearly elucidated. In this study, we investigated the anti-cancer effects of water extract of Dendropanax morbifera (DP) on 2 human breast cancer cell lines (estrogen dependent MCF-7 and estrogen independent MDA-MB-231). Methods : The MTT assay and flow cytometry were used to assess cell proliferation, along with cell cycle analysis. Nitric oxide production was detected by Griess assay. The expression of apoptosis related gene was assessed by quantitative real-time PCR. Results : Our data revealed that DP inhibits the cell growth in a dose dependent manner (0, 50, 100, 250, and 500 μg/ml) of both estrogen independent MDA-MB-231 and estrogen dependent MCF-7 breast cancer cells. Also, LPS induced nitric oxide production was significantly reduced by DP. Cell cycle analysis showed an increased G1 phase in the MCF-7 cell and G2/M phase in the MDA-MB-231 cell. DP decreased mRNA expression of apoptotic suppressor gene Bcl-xL, and increased mRNA expression of pro-apoptotic genes. DP increased mRNA expression of p21, and Rip1 in both cell. And DP decreased mRNA expression of survivin in the MCF-7 cell. Conclusions : Taken together, these results indicate that DP extract are source of anti-cancer potential and could be developed botanical drug.
Keywords
Dendropanax morbifera; breast cancer; anticancer; MCF-7; MDA-MB-231;
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1 Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol. 2007;35(4):495-516.   DOI   ScienceOn
2 Yu MH, et al. Induction of Apoptosis by Immature Prunus salicina Lindi. cv. Soldam. Korean J food Sci Thechnol. 2005;37(2):221-7.
3 Gross A, McDonnell JM, Korsmeyer SJ. Bcl-2 family members and the mitochondria in apoptosis. Genes. 1999;13(15):1899-911.   DOI   ScienceOn
4 Yang J, et al. Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked. Science. 1997;275(5303):1129-32.   DOI   ScienceOn
5 Boatright KM, Salvesen GS. Mechanisms of caspase activation. Curr Opin Cell Biol. 2003;15(6):725-31.   DOI   ScienceOn
6 Sah NK, et al. Structural, functional and therapeutic biology of survivin. Cancer Letters. 2006;244(2):164-71.   DOI   ScienceOn
7 Zhang D, Lin J, Han J. Receptor-interacting protein (RIP) kinase family. Cellular & Molecular Immunology. 2010;7:243-9.   DOI   ScienceOn
8 Lee JW, et al. Induction of human leukemia U937 cell apoptosis by an ethanol extract of Dendropanax morbifera Lev. through the caspase-dependent pathway. Oncol Rep. 2013;30(3):1231-8.   DOI
9 Kim HR, Chung HJ. Chemical Characteristics of the Leaves and the Seeds of Korea Dendropanax. J. Korean Soc. Agric. Chem. Biotechnol. 2000;43(1):63-6.
10 An DK. A color-illustrated guide to Korean herb. Seoul:Kyohak. 1998:349.
11 Hyun TK, et al. Evaluation of anti-oxidant and anti-cancer properties of Dendropanax morbifera Leveille. Food chemistry. 2013;141(3):1947-55.   DOI   ScienceOn
12 Moon HI. Antidiebetic effects of dendropanoxide from leaves of Dendropanax morbifera Leveille in normal and streptozotocin-induced diabetic rats. Hum Exp Toxicol. 2011; 30(8):870-5.   DOI   ScienceOn
13 Park SA, et al. Cellular Antioxidant Activity and Whitening Effects of Dendropanax morbifera Leaf Extracts. Korean J. Microbiol. Biotechnol. 2013; 41(4):407-15.   DOI   ScienceOn
14 Lee SH, et al. Screening of Immune Activation Activities in the Leaves of Dendropanax morbifera Lev. Korean J. Medicinal Crop Sci. 2002;10(2):109-15.
15 Korea central cancer registry, Ministry of Health&wealfare : Annual report of cancer statistics in Korea in 2011. 2013. Available from URL:http://meta.narastat.kr/metasvc/index.do?confmNo=11744&inputYear=2011. Accessed September 1st, 2014.
16 Lee JJ. The Cytotoxic and Hormonal Treatment for Breast Cancer. Kyung Hee Univ Med Cent. 2004;20(2):92-6.
17 Dorssers LC, et al. Tamoxifen resistance in breast cancer. Elucidation mechaniam. Drug. 2001;61:1721-33.
18 Shanmugam MK, et al. Targeted inhibition of tumor proliferation, survival, and metastasis by pentacyclic triterpenoids: potential role in prevention and therapy of cancer. Cancer letters. 2012;320(2):158-70.   DOI   ScienceOn
19 Navindra P, Seeram NP. Berry fruit for cancer prevention; Current status and future prospects. J Agric Food Chem. 2008;56:630-5.   DOI   ScienceOn
20 Yoon WK, Kim DC. Toosendan Fructus Induces Apoptotic Cell Death in MCF-7 Cell, Via the Inhibition of Bcl-2 Expression. The journal of Korean obstetrics & gynecology. 2008;21(3) :18-33.
21 Zhou J, Zhong Y. Breast cancer immunotherapy. Cell Mol Immunol. 2004;1(4):247-55.
22 Korean obstetrics & gynecology. Oriental obstetrics & gynecology(下). Seoul: Euiseongdang. 2012:399.
23 Jeong KA, Park KM, Cho SH. Anti-proliferative effects of Sam-nueng (Sparganii Rhizoma) extract on MCF-7 cells. The journal of korean obstetrics & gynecology. 2006;19(1):166-77.
24 Cho SI, et al. Inhibition of Cellular Proliferation by CURCUMAE LONGAE Rhizoma Extracts on MCF-7. Kor. J. Herbology. 2006;21(1):71-7.
25 Park YA, Kim DC. Corydalis Tuber Induces Apoptosis in MCF-7 Cells, Via Inhibition of Bcl-2 and Bcl-xL Expression. The journal of korean obstetrics & gynecology. 2008;21(4) :90-103.
26 Cho JK. The clinical oncology in oriental medicine. Korea:Joomin publisher. 2001:119-20.
27 Lee JW, et al. Dendropanoxide induces autophagy through ERK1/2 activation in MG-63 human osteosarcoma cells and autophagy inhibition enhances dendropanoxide-induced apoptosis. PLoS One. Dec 17 2013;8(12):e83611. Available from:URL:http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0083611.   DOI
28 Levy SM, et al. Prognostic risk assessment in primary breast cancer by behavioral and immunological parameters. Health Psychol. 1985; 4(2):99-113.   DOI   ScienceOn
29 Jeong BS, et al. Studies on the Distribution of Dendropanax morbifera and Component Analysis of the Golden Lacquer. Korean J. Biotechnol. Bioeng. 1995;10(4):393-400.
30 Jin CY, et al. Oleifolioside B-mediated autophagy promotes apoptosis in A549 human non-small cell lung cancer cells. Int J Oncol. 2013;43(6):1943-50.   DOI
31 Yu HY, et al. Oleifolioside A, a New Active Compound, Attenuates LPS-Stimulated iNOS and COX-2 Expression through the Downregulation of NF-κB and MAPK Activities in RAW 264.7 Macrophages. Evid Based Complement Alternat Med. 2012. Available from: URL:http://www.hindawi.com/journals/ecam/2012/637512/.
32 Yu HY, et al. Oleifolioside A mediates caspase-independent human cervical carcinoma HeLa cell apoptosis involving nuclear relocation of mitochondrial apoptogenic factors AIF and EndoG. J Agric Food Chem. 2012;60(21): 5400-6.   DOI   ScienceOn
33 Lee SN, Kang KJ. The Effect of blueberry extract on gene expression related to apoptosis in human breast cancer cells. J. East Asian Soc. Dietary Life. 2010;20(1):30-6.
34 Macchiarini P, et al. Relation of neovascularization to metastasis of non-small-cell-lung cancer. Lancet. 1992;340(8812):145-6.   DOI   ScienceOn
35 Wink DA, et al. Chemical biology of nitric oxide: regulation and protective and toxic mechanisms. Curr. Top. Cell Regul. 1996;34:159-87.   DOI
36 Ambs S, et al. Relationship between p53 mutation and inducible nitric oxide synthases expression in human colorectal cancer. J. Natl. Cancer Inst. 1999;91(1):86-8.   DOI   ScienceOn
37 Lauren Pecorino. Molecular Biology of Cancer. 2nd ed. Seoul:Hanmi. 2009 :239-62.
38 Yang XH, Edgerton S, Thor AD. Reconstitution of caspase-3 sensitized MCF-7 breast cancer cells to radiation therapy. Int J oncol. 2005;26(6): 1675-80.
39 Lauren Pecorino. Molecular Biology of Cancer : Mechanisms, Targets, and Therapeutics. 3rd ed. Oxford:Oxford University Press. 2012:136-7.