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http://dx.doi.org/10.15204/jkobgy.2014.27.2.034

Activation of Immune System & Antimetastatic Effects of Ojeok-san by Oral Administration  

Lee, Mi-Joo (Dept. of Korean Gynecology, College of Korean Medicine, Kyung-Hee University)
Hwang, Deok-Sang (Dept. of Korean Gynecology, College of Korean Medicine, Kyung-Hee University)
Lee, Jin-Moo (Dept. of Korean Gynecology, College of Korean Medicine, Kyung-Hee University)
Jang, Jun-Bock (Dept. of Korean Gynecology, College of Korean Medicine, Kyung-Hee University)
Lee, Kyung-Sub (Dept. of Korean Gynecology, College of Korean Medicine, Kyung-Hee University)
Lee, Chang-Hoon (Dept. of Korean Gynecology, College of Korean Medicine, Kyung-Hee University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.27, no.2, 2014 , pp. 34-45 More about this Journal
Abstract
Objectives: This study was designed to investigate intestinal immune system activation and antimetastatic effect of Ojeok-san on cancer cells by oral administration. Methods: Cell viability of Ojeok-san was tested with colon 26-M3.1 carcinoma cells and Peyer's patch cells in vitro. Antimetastatic experiments were conducted in vivo mouse model by using colon 26-M3.1 carcinoma cell. To observe immunomodulating effects of Ojeok-san on Peyer's patch cells, we measured interleukin (IL)-4, GM-CSF. In addition to observing effects of Ojeok-san on hematopoiesis, we measured proliferation of bone marrow cells mediated by Peyer's patch cells in vitro. IgA induction activated in serum and intestinal content was measured to observe the effect of orally administered Ojeok-san on mucosal immune system. After administering Ovalbumin (OVA) with Ojeok-san, Proliferation of Peyer's patch cell was measured to investigate gut immunostimulatory effect. Results: in vitro cytotoxicity analysis, the inhibitory concentration $(IC)_{50}$ of the colon 26-M3.1 carcinoma cell was $890{\mu}g/ml$. $IC_{50}$ of the Peyer's patch cells with LPS was $990{\mu}g/ml$. We found that orally administered Ojeok-san significantly inhibited tumor metastasis in vivo. In addition, the amounts of IL-4 and GM-CSF in the culture supernatant of Peyer's patch cells were significantly increased compared to the control group. The proliferation of bone marrow cell was significantly up-regulated with Ojeok-san. These results indicate that oral administration of Ojeok-san enhances the secretion of hematopoietic growth factors such as GM-CSF and IL-4 from Peyer's patch cells, and these cytokines also act on modulator of bone marrow cell proliferation. After orally administering Ovalbumin (OVA) with Ojeok-san, IgA induction and Proliferation of peyer's patch cell was up-regulated with Ojeok-san. These results means orally administered Ojeok-san activates intestinal immune system and has an inhibitory effect on tumor metastasis. Conclusions: Orally administered Ojeok-san appears to have considerable activity on the anti-metastasis by activation of immune system.
Keywords
Ojeok-san; Cancer; Anti-Metastasis; Complementary Therapies; Immune System;
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Times Cited By KSCI : 9  (Citation Analysis)
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