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http://dx.doi.org/10.15204/jkobgy2014.27.1.017

Osteoclast Differentiation of Polygoni Cuspidati Radix Extracts Effects and Mechanism of Inhibition Studies  

Jang, Hee-Jae (Dept. of Gynecology, College of Korean Medicine, Kyung-Hee University)
Hwang, Deok-Sang (Dept. of Gynecology, College of Korean Medicine, Kyung-Hee University)
Lee, Jin-Moo (Dept. of Gynecology, College of Korean Medicine, Kyung-Hee University)
Lee, Chang-Hoon (Dept. of Gynecology, College of Korean Medicine, Kyung-Hee University)
Lee, Kyung-Sub (Dept. of Gynecology, College of Korean Medicine, Kyung-Hee University)
Jang, Jun-Bok (Dept. of Gynecology, College of Korean Medicine, Kyung-Hee University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.27, no.1, 2014 , pp. 17-27 More about this Journal
Abstract
Objectives: This study was conducted to evaluate the inhibitory effect of polygoni cuspidati radix (PCR) extract on osteoclast differentiation. Methods: MTT-assay was performed to estimate cytotoxicity of PCR extract in BMMs stimulated with RANKL. Tartrate resistant acid phosphatase (TRAP) staining, TRAP activity and RT-PCR were performed to know the inhibitory effect on osteoclast differentiation. actin ring formation were analysed to observe the effect of PCR extract. Results: PCR decreased the number of TRAP positive cells and TRAP activities in BMMs stimulated with RANKL and M-CSF. PCR restrained the formation of actin ring. PCR down regulated the induction of NFATc1, c-Fos, TRAP and OSCAR by RANKL. PCR inhibited NF-${\kappa}B$ activity by inducing degradation of $I{\kappa}B{\alpha}$. Conclusions: We suggest that PCR Extracts can be an effective therapeutic agent on osteoclast differentiation caused by diseases such as osteoporosis.
Keywords
Polygoni Cuspidati Radix Extracts (PCR Extracts); Osteoclast; Differentiation; Tartrate Resistant Acid Phosphatase (TRAP) Activity; NF-${\kappa}B$; C-FOS; NFATc1;
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Times Cited By KSCI : 7  (Citation Analysis)
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