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http://dx.doi.org/10.15204/jkobgy.2012.25.1.001

Inhibitory Effects of Achyranthis Radix Extract Mixed with Hydrogel on Osteoclast Differentiation  

Choi, Jin-Young (Dept. of Oriental Gynecology, college of Oriental Medicine, Kyung-Hee University)
Lee, Chang-Hoon (Dept. of Oriental Gynecology, college of Oriental Medicine, Kyung-Hee University)
Jang, Jun-Bock (Dept. of Oriental Gynecology, college of Oriental Medicine, Kyung-Hee University)
Lee, Kyung-Sub (Dept. of Oriental Gynecology, college of Oriental Medicine, Kyung-Hee University)
Lee, Jin-Moo (Dept. of Oriental Gynecology, college of Oriental Medicine, Kyung-Hee University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.25, no.1, 2012 , pp. 1-10 More about this Journal
Abstract
Purpose: This study was conducted to evaluate the inhibitory effect of Achyranthis Radix extract(ARE) loaded hydrogel on osteoclast differentiation. Methods: MTT-assay was performed to estimate cytotoxicity of ARE, Achyranthis Radix-alginate hydrogel disk(ARHD) in bone marrow macrophages stimulated(BMMs) with human receptor activator of nuclear factor-${\kappa}B$ ligand(RANKL), human macrophage-colony stimulating factor(M-CSF). Tartrate resistant acid phosphatase staining and RT-PCR were performed to know the inhibitory effect on osteoclast differentiation. Reactive oxygen species and actin ring formation were analysed to observe the effect of ARHD. Results: ARE has no cytotoxicity at the concentration of 0.1 $mg/m{\ell}$ or lower. ARE decreased the number of TRAP positive cells in RANKL, M-CSF stimulated BMMs and the gene expression. ARHD has no cytotoxicity at the concentration of 10 ${\mu}g/m{\ell}$ (24, 48hours), 50 ${\mu}g/m{\ell}$ (24 hours). ARHD restrained the synthesis of reactive oxygen species and the formation of actin ring. Conclusions: Achyranthis Radix has the inhibitory effect of osteoclast differentiation and bone resorption. Further studies are needed to treat osteoporosis by Achyranthis Radix.
Keywords
Achyranthis Radix; Osteoclast differentiation; Hydrogel; RANKL;
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