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http://dx.doi.org/10.15204/jkobgy.2011.24.4.001

Inhibitory Effects of Wogonin Mixed with Hydrogel on Osteoclast Differentiation  

Yang, Na-Rae (Dept. of Gynecology, College of Oriental Medicine, Kyung-Hee University)
Lee, Jin-Moo (Dept. of Gynecology, College of Oriental Medicine, Kyung-Hee University)
Lee, Chang-Hoon (Dept. of Gynecology, College of Oriental Medicine, Kyung-Hee University)
Jang, Jun-Bock (Dept. of Gynecology, College of Oriental Medicine, Kyung-Hee University)
Lee, Kyung-Sub (Dept. of Gynecology, College of Oriental Medicine, Kyung-Hee University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.24, no.4, 2011 , pp. 1-9 More about this Journal
Abstract
Purpose: Wogonin is an active component isolated from scutellariae radix. This study was conducted to evaluate the inhibitory effect of wogonin mixed with hydrogel on osteoclast differentiation. Methods: It was performed to estimate cytotoxicity of Wogonin alginate hydrogel disk(WHD) in BMMs stimulated with RANKL, M-CSF. ROS synthesis and actin ring formation were analysed to observe the effect of WHD. Results: WHD has no cytotoxicity at the concentration of 0.1 ${\mu}g/ml$ or lower. 0.1 ${\mu}g/ml$, 1 ${\mu}g/ml$ WHD restrained the synthesis of ROS and 0.1 ${\mu}g/ml$, 1 ${\mu}g/ml$ WHD restrained the formation of actin ring. Conclusions: WHD has the inhibitory effect of osteoclast differentiation and bone resorption. Further studies are needed to treat osteoporosis by herbal medicine.
Keywords
Scutellariae Radix; Wogonin alginate hydrogel disk; osteoclast; hydrogel; RANK ligand;
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Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 김동일. 2007년 7월 폐경후 여성의 호르몬 요법에 대한 WHI 연구 발표 이 후 한의학은 어떤 기여를 할 수 있을까?. 대한한방부인과학회지. 2004;17(3) :105-115.
2 강병문. 골다공증 치료의 의학약제와 대체 약물의 비교. 대한산부회지. 2006; 49(12):2459-2473.
3 박형무. 한국의 골다공증 약제의 사용 현황. 대한산부회지. 2010;53(2):152-159.
4 손계순. 콩 섭취가 폐경 후 여성의 골밀도 및 골대사 지표에 미치는 영향. 대한간호학회지. 2006;36(6):933-941.
5 김정근 등. 숙지황과 가시오가피 추출물이 조골세포의 활성과 증식 및 파골세포의 골흡수 억제에 미치는 효과. 대한골대사학회지. 2002;9(2):133-143.
6 김희진, 이태균. 폐경기골다공증에 관한 문헌적 고찰. 대한한방부인과학회지. 1998;11(1):131-148.
7 김진혁, 유동열. 골다공증에 응용되는 수종의 강근골 약물에 문헌적 고찰. 대한한방부인과학회지. 1999;12(1):105-124.
8 허준. 대역 동의보감. 서울:동의보감출판사. 2005:770.
9 전국한의과대학 본초학교수 공편저. 본초학. 서울:영림사. 2004:178-9.
10 신정민 등. 황금 추출물이 조골세포와 파골세포의 활성에 미치는 영향. 한국식품과학회지. 2008;40(6):674-679.
11 Yoo HS, Lee EA, Park TG. Doxorubicin -conjugated biodegradable polymeric micelles having acid-cleavage linkages. J Control Release. 2002;82:17-27.   DOI   ScienceOn
12 정호연. 골다공증 진단 및 치료 지침 2007. 대한내분비학회지. 2008;23(2) :76-108.
13 김현주. 글루코코르티코이드에 의해 유도되는 골다공증의 병리학적 기전. 대한내분비학회지. 2009;24(4):231-236.
14 오세인 등. 제 1형 골다공증에서 멜라토닌이 흰쥐 경골의 항산화효소 활성에 미치는 영향. 한국노화학회지. 2001;11(3):29-35.
15 2009년 생명표. 한국통계청. 2010;http ://kostat.go.kr/.
16 이효정 등. 천연물 추출물이 파골세포분화억제 효과 검색. 한국식품과학회지. 2005;37(6):997-1004.
17 신민교 편저. 임상본초학. 서울:영림사. 2002:400-401.
18 Chena YC et al. Wogonin, baicalin, and baicalein inhibition of inducible nitric oxide synthase and cyclooxygenase-2 gene expressions induced by nitric oxide synthase inhibitors and lipopolysaccharide. Biochem Pharmacol. 2001;61(11) :1417-1427.   DOI
19 Krakauer T, Li B, Young H. The flavonoid baicalin inhibits superantigen -induced inflammatory cytokines and chemokines. FEBS Lett. 2001;500(1-2) :52-55.   DOI   ScienceOn
20 Jerome AR, Kim BG. Melatonin promotes osteoblast differentiation and bone formation. J Biol Chem. 1999;274(31):22041-22047.   DOI
21 Gao Z et al. Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi. Biochim Biophys Acta(BBA). 1999;1472(3):643-650.   DOI   ScienceOn
22 Liaw J, Gau YY, Chao YC. Effect of baicalin on tracheal permeability in ovalbumin(OA)-sensitized guinea pigs. Pharm Res. 1999;16(10):1653-1657.   DOI   ScienceOn
23 You KM, Jong HG, Kim HP. Inhibition of cyclooxygenase/lipoxygenase from human platelets by polyhydroxylated /methoxylated flavonoids isolated from medicinal plants. Arch Pharm Res. 1999;22(1):18-24.   DOI
24 Guoa Q et al. Anti-hepatitis B virus activity of wogonin in vitro and in vivo. Antiviral Res. 2007;74(1):16-24.   DOI   ScienceOn
25 Gao Z, Huang K, Xu H. Protective effects of flavonoids in the roots of scutellaria baicalensis georgi against hydrogen peroxide-induced oxidative stress in hs-sy5y cells. Pharmacol Res. 2001;43(2):173-178.   DOI   ScienceOn
26 Cho J, Lee HK. Wogonin inhibits excitoxic and oxidative neuronal damage in primary cultured rat cortical cells. Eur J Pharmacol. 2004;485(1-3) :105-110.   DOI
27 Lim BO. Effects of wogonin, wogonoside and 3, 5, 7, 2', 6' - pentahydroxyflavone on chemical mediator production in peritoneal exduate cells and immunoglobulin E of rat mesenteric lymph node lymphocytes. J Ethnopharmacol. 2003;84(1):23-29.   DOI   ScienceOn
28 Kim HC et al. The plant flavonoid wogonin suppresses death of activated C6 rat glial cells by inhibiting nitric oxide production. Neurosci Lett. 2001; 309(1):67-71.   DOI   ScienceOn
29 Kim YO et al. Cytoprotective effect of Scutellaria baicalensis in CA1 hippocampal neurons of rats after global cerebral ischemia. J Ethnopharmacol. 2001;77(2-3):183-188.   DOI
30 Wakabayashi I, Yasui K. Wogonin inhibits inducible prostaglandin E(2) production in macrophages. Eur J Pharmacol. 2000;406(3):477-481.   DOI   ScienceOn
31 Ma S et al. Antiviral Chinese medicinal herbs against respiratory syncytial virus. J Ethnopharmacol. 2002;79(2) :205-211.   DOI   ScienceOn
32 이성수 등. 건강한 폐경 후 여성에서 혈청 산화스트레스 표식자와 골밀도 및 골대사와의 상관 관계. 대한골대사학회지. 2005;12(2):171-177.
33 Hui KM et al. Anxiolytic effect of wogonin, a benzodiazepine receptor ligand isolated from Scutellaria baicalensis Georgi. Biochem Pharmacol. 2002;64(9):1415-1424.   DOI   ScienceOn
34 Kang SS et al. Neuroprotective effects of flavones on hydrogen peroxide induced apoptosis in SH-SY5Y neuroblostoma cells. Bioorg Med Chem Lett. 2004;14(9):2261-2264.   DOI   ScienceOn
35 Hoffman AS. Hydrogels for biomedical applications. Adv Drug Deliv Rev. 2002;43:3-12.
36 조형래 등. 폐경후기 여성들이 호르몬 보충요법. 대한한방부인과학회지. 2002; 15(3):75-81.
37 대한산부인과학회 교과서편찬위원회. 부인과학 제4판. 서울:도서출판 고려의학. 2008:563-567.
38 Lee NK et al. A crucial role for reactive oxygen species in RANKL-induced osteoclast differentiation. Blood. 2005; 106(3):852-859.   DOI   ScienceOn
39 Ha H et al. Reactive oxygen species mediate RANK signaling in osteoclasts. Exp Cell Res. 2004;301(2):119-127.   DOI   ScienceOn
40 오성렬 등. 골 흡수에 M-CSF의 효과. 대한골대사학회지. 2009;16(1):15-21.