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http://dx.doi.org/10.13106/jwmap.2022.Vol5.no3.1

A Study for Antimicrobial Susceptibility of Wetlands to Eliminate Toilet Bacteria  

LEE, Woo-Sik (Department of Chemical & Biological Engineering, Gachon University)
KWON, Woo-Taeg (Department of Environmental health and Safety, Eulji University)
Publication Information
Journal of Wellbeing Management and Applied Psychology / v.5, no.3, 2022 , pp. 1-5 More about this Journal
Abstract
Purpose: The purpose of this study is to investigate whether wetland has antimicrobial activity on pathogenic bacteria in the toilet bowl. Research design, data and methodology: Air-dried mud obtained from "Jilmoe Bog" wetland was packed and dissolved in the autoclaved saline. Antimicrobial susceptibility was assessed against three Gram-negative bacteria using disk diffusion method and broth dilution method. Identification of specific bacterium presented in wetland supernatant was performed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Results: Incubation of three Gram-negative bacteria with wetland supernatant inhibited bacterial growth of the bacteria, otherwise increased prevalence of specific bacterium. It was confirmed that Pseudomonas putida was presented in wetland supernatant. Conclusions: The results presented in this study might provide the possibility to utilize wetland supernatant as a bioremediation of toilet bowl bacteria.
Keywords
Antimicrobial susceptibility; Wetland; Disk diffusion method; Broth dilution method; Toilet hygiene;
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