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http://dx.doi.org/10.5806/AST.2020.33.6.233

Simultaneous determination of 9 preservatives in processed foods using high-performance liquid chromatography with photo diode array detector  

Lee, Do-Yeon (Department of Research & Development, Fore Front TEST)
Kim, Min-Hee (Department of Research & Development, Fore Front TEST)
Ahn, Jang-Hyuk (Department of Research & Development, Fore Front TEST)
Publication Information
Analytical Science and Technology / v.33, no.6, 2020 , pp. 233-239 More about this Journal
Abstract
This study was performed to develop an analytical method using Carrez reagents as the precipitant to effectively and easily remove proteins and lipids while pretreating samples for the simultaneous determination of preservatives, including dehydroacetic acid (DHA), sorbic acid (SA), benzoic acid (BA), methyl ρ-hydroxybenzoate (MP), ethyl ρ-hydroxybenzoate (EP), propyl ρ-hydroxybenzoate (PP), isopropyl ρ-hydroxybenzoate (IPP), butyl ρ-hydroxybenzoate (BP), and isobutyl ρ-hydroxybenzoate (IBP). The effective selectivity was determined by HPLC separation analysis for nine preservatives in the test solution, after removing interfering materials such as lipids and proteins. The method developed in this study showed excellent linearity at 0.999 or higher. The limit of detection (LOD) ranged from 0.09 to ~0.12 mg/L and the limit of quantitation (LOQ) was ~0.280.37 mg/L. The results of the recovery test on processed foods, including pickles, cheeses, processed meat products, beverages, sauces, and emulsified foods showed DHA, SA, BA, MP, EP, IPP, PP, IBP, and BP at 90.9~107.7 %, 85.4~113.7 %, 90.7~111.6 %, 84.5~111.2 %, 81.3~110.9 %, 82.5~102.2 %, 81.1~110.0 %, 80.9~109.0 %, and 82.4~110.3 %, respectively. The probability of the simultaneous analytical method developed in this study as a quantitative method was confirmed for various processed foods.
Keywords
preservatives; carrez reagent; simultaneous determination; processed foods;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 MFDS. Korean food additives codes, Standards and specifications of food additives. Ministry of Food and Drug Safety Chungcheongbuk-do, 2019.
2 MFDS. Korean food codes, Standards and specifications of foods. Ministry of Food and Drug Safety Chungcheongbuk-do, 2019.
3 A. International, 'Office Method of Analysis of AOAC international', AOAC Internatioinal, 2012.
4 M. Gonzalez, M. Gallego and M. Valcarcel, Journal of Chromatography A, 823(1-2), 321-329(1998).   DOI
5 N. J. Russell and G. W. Gould, 'Food preservatives', Springer Science & Business Media, 2003.
6 C. V. Salmond, R. G. Kroll and I. R. Booth, Microbiology, 130(11), 2845-2850 (1984).   DOI
7 H.-J. Lin and Y.-M. Choong, J. Food Drug. Anal., 7(4) (1999).
8 I. O. f. Standardization (ISO), 'Milk and milk products - Determination of the benzoic and sorbic acid contents', 2008.
9 H. Hsi-Ya, C. Chia-Ling, C. Chen-Wen and Y. JuiMing, Food Chemistry, 89(2), 315-322 (2005).   DOI
10 MFDS. Guidelines on standard procedures for preparing analysis methods. Ministry of Food and Drug Safety Chungcheongbuk-do, 2016.
11 MFDS. Korean pharmaceutical method validation guideline. Ministry of Food and Drug Safety Chungcheongbuk-do, 2015.
12 B. Saad, M. F. Bari, M. I. Saleh, K. Ahmad and M. K. M. Talib, Journal of Chromatography A, 1073(1-2), 393-397(2005).   DOI
13 A. International, 'Office Method of Analysis of AOAC international', AOAC Internatioinal, 2012.
14 E.-R. Park, S.-K. Lee, H.-S. Hwang, C.-S. Mun, I.-S. Gwak, O.-H. Kim and K.-H. Lee, Journal of the Korean Society of Food Science and Nutrition, 37(12), 1640-1646 (2008).   DOI
15 K.-H. Lee, A.-Y. Kim, S.-H. Choi, H. S. Lim, J.-C. Choi, M.-H. Kim, S.-H. Kim and S.-D. Ha, J. Fd. Hyg. Safety, 28(4), 293-298 (2013).   DOI
16 R. Sieber, U. Bütikofer and J. Bosset, International Dairy Journal, 5(3), 227-246 (1995).   DOI
17 N. Koyuncu and V. Uylaser, Asian Journal of Chemistry, 21(6), 4901-4908 (2009).