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http://dx.doi.org/10.5806/AST.2015.28.3.196

Analysis of selenoaminoacids and selenoproteins in blood serum of sows fed by selenium fortified feed  

Park, Myungsoon (Department of Chemistry Education, Korea National University of Education)
Lee, Sung Hoon (Livestock Experiment Station, Gyungsangnamdo Livestock production Research Institute)
Pak, Yong-Nam (Department of Chemistry Education, Korea National University of Education)
Publication Information
Analytical Science and Technology / v.28, no.3, 2015 , pp. 196-203 More about this Journal
Abstract
Selenium species (inorganic selenium, selenoaminoacids, and selenoproteins) were analyzed using anion exchange and affinity chromatography, which were connected to ICP/MS for the blood serum of sows fed by seleniumfortified feed. The Anion Exchange PRP X-100 column was used for the analysis of inorganic selenium (Se4+ and Se6+) and selenoaminoacids. The HEP column was used to separate SelP from GPx+SeAlb in selenoproteins. A quantitative analysis was performed using the post-column isotope dilution technique. The lactating sows were divided into three groups and fed by selenium fortified feed (organic 0.3 mg/kg, 0.6 mg/kg and inorganic 0.6 mg/kg) for four weeks. The test groups showed increases in selenoaminoacids compared with the control group, except the inorganic feed group. There was no significant difference between the organic feed groups. All test groups showed increases in selenoproteins. In particular, SelP showed a large increase that was 1.5 times higher than the other proteins.
Keywords
sow blood serum; AE & AF HPLC-ICP/MS; selenoproteins; selenoaminoacids; selenium fortified feed;
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1 H. H. Kim, H. Yang and H. Y. Kim, Korean J. Nutr., 44(2), 112-118 (2011).   DOI   ScienceOn
2 Y. Park, I.-S. Kim, S. H. Lee, S. H. lee, D. W. Jung, C. J. Park and Y. M. Kim, J. Life Sci., 2(1), 55-61 (2012).
3 I. H. Jo, S. H. Lee, G. W. Kim, S. Hwangbo, O. K. Han, T. H. Song, T. I. Park and I. B. Choi, J Korean Soc Grassl Forage Sci., 32(1), 39-48 (2012).   DOI   ScienceOn
4 Y. D. Jang, H. B. Choi, S. Durosoy, P. Schlegel, B. R. Choi and Y. Y. Kim, Asian-Aust. J. Anim. Sci., 23(7), 931-936 (2010).   DOI   ScienceOn
5 J. Travnicek, L. Pisek, I. Herzig, J. Doucha, H. Kvicala, V. Kroupova and H. Rodinova, Veterinarni Medicina, 52, 42-48 (2007).
6 J. S. Jung, J. H. Lee and Y. N. Pak, Bull. Korean Chem. Soc., 34(12), 3817-3824 (2013).   DOI   ScienceOn
7 H. A. Meyer, T. Endermann, C. Stephan, M. Stoedter, T. Behrends, I. Wolff, K. Jung and L. Schomburg, PLOS ONE, issue 10, e46644 (2012).
8 J. Y. Ahn, H. Kwon and Y. N. Pak, Analytical Sci. & Technol., 27(2), 357-363 (2014).   DOI   ScienceOn
9 A. J. Duffield-Lillico, E. H. Slate and M. E. Reid, J. Natl Cancer Inst., 95(19), 1477-1481 (2003).   DOI   ScienceOn
10 D. Juresa, J. Darrouze’s, N. Kienzl, M. Bueno, F. Pannier, M. Potin-Gautier, A. Kevin and D. Kuehnelt, J. Anal. At. Spectrom., 21, 684-690 (2006).   DOI   ScienceOn
11 E. A. Klein, I. M. Thompson and S. M. Lippman, Prostate Cancer Prostatic Dis., 3(3), 145-151 (2000).   DOI
12 S. M. Lippman, E. A. Klein and P. J. Goodman, JAMA, 301(1), 39-51 (2009).   DOI
13 Z. Pedrero and Y. Madrid, Anal. Chim. Acta, 634, 135-152 (2009).   DOI   ScienceOn
14 P. Jitaru, C. Giulio, G. Andrea, C. Paolo and B. Carlo, Anal Bioanal Chem., 391, 661-669 (2008).   DOI
15 M. Roman, P. Jitaru and C. Barbante, Metallomics, 6, 25-54 (2014).   DOI
16 D. H. Vanesa, L. F. S. Maria and S. M. Alfredo, Analytica Chimica Acta, 538, 99-105 (2005).   DOI   ScienceOn
17 F. A. Aborode, A. Raab, S. Foster, E. Lombi, W. Maher, E. M. Kruppa and J. Feldmann, Metallomics, 7(3), Mar 9, in press, (2015).
18 K. Bierla, J. Bianga, L. Ouerdane, J. Szpunar and A. Yiannikouris, R. Lobinski, J. Proteomics., 87, 26-39 (2013).   DOI   ScienceOn
19 J. Bianga, E. Govasmark and J. Szpunar, Anal Chem., 85(4), 2037-2043 (2013).   DOI   ScienceOn
20 S. Palioura, R. L. Sherrer, T. A. Steitz, D. Söll and M. Simonovic, Science, 325, 321-325 (2009).   DOI   ScienceOn
21 F. Ursini and S. Heim, M. Kiess, Science, 285, 1393-1396 (1999).   DOI   ScienceOn
22 L. H. Foster and S. Sumar, Crit Rev Food Sci Nutr., 37(3), 211-228 (1997).   DOI   ScienceOn
23 H.-Y. Jang, H. Min, J. Lee and Y.-N. Pak, Analytical Sci. & Technol., 26(3), 182-189 (2013).   DOI   ScienceOn
24 T. Celine, R. Ann, D. T. Ludwig, S. Yves-Jacques and P. Luc, Food Chemistry, 130, 767-784 (2012).   DOI   ScienceOn
25 O. S. Kwon, J.-W. Hong, B.-J. Min, W.-B. Lee, K.-S. Shon, I.-H. Kim and J.-M. Kim, J. Korean Soc. Food Sci Nutr., 33(7), 1206-1211 (2004).   DOI   ScienceOn
26 J. Bianga, Z. Touat-Hamici, K. Bierla, S. Mounicou, J. Szpunar, L. Chavatte and R. Lobinski, J Proteomics., 108, 316-24 (2014).   DOI   ScienceOn