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http://dx.doi.org/10.5806/AST.2015.28.2.117

Establishment and application of a qualitative real-time polymerase chain reaction method for detecting genetically modified papaya line 55-1 in papaya products  

Kwon, Yu Jihn (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration)
Chung, So Young (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration)
Cho, Kyung Chul (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration)
Park, ji Eun (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration)
Koo, Eun Joo (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration)
Seo, Dong Hyuk (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration)
Kim, Eugene (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration)
Whang, Jehyun (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration)
Park, Seong Soo (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration)
Choi, Sun Ok (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration)
Lim, Chul Joo (Hazardous Substances Analysis Team, Center for Food and Drug Analysis, Gyeongin Regional Food and Drug Administration)
Publication Information
Analytical Science and Technology / v.28, no.2, 2015 , pp. 117-124 More about this Journal
Abstract
Genetically modified (GM) papaya line 55-1, which is resistant to PRSV infection, has been marketed globally. Prompt and sensitive protocols for specific detections are essential for the traceability of this line. Here, an event- and construct-specific real-time polymerase chain reaction (RT-PCR) method was established to detect 55-1. Qualitative detection was possible for fresh papaya fruit up to dilutions of 0.005% and 0.01% for the homozygous SunUp and heterozygous Rainbow cultivars, respectively, in non-GM papaya. The method was applied in the qualitative detection of 55-1 in eight types of commercially processed papaya products. Additionally, papaya products were monitored to distinguish GM papaya using the P35S and T-nos RT-PCR detection methods. As expected, detection capacity was improved via modified sample preparation and the established RT-PCR detection method. Taking these results together, it can be suggested that a suitable method for the extraction and purification of DNA from processed papaya products was established for the detection of GM papaya.
Keywords
genetically modified; real-time polymerase chain reaction; papaya; 55-1; monitoring;
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