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http://dx.doi.org/10.5806/AST.2014.27.6.277

Effect of concentration, pressure, and cut-off size on removing phospholipase A2 in bee venom by ultrafiltration  

Lee, Ji-Yeon (Chungjin Biotech, Hanyang University)
Kim, Il Kwang (N. CosmeCeutical Co., Ltd., Wonkwang Univ.)
Lee, Jong Soo (College of Veterinary Medicine, Chungnam National University)
Kim, Eui Kyung (Department of Pharmacology and Toxicology, College of Veterinary Medicine, Gyeongsang National University)
Kim, Choul-Goo (Chungjin Biotech, Hanyang University)
Publication Information
Analytical Science and Technology / v.27, no.6, 2014 , pp. 277-283 More about this Journal
Abstract
The effects of concentration, pressure, and molecular sige on removing allergenic substance (phospholipase $A_2$) in bee venom by ultrafiltration were investigated. The membrane pore sizes were selected based on the molecular weight of the main compounds. The conditions of concentration and pressure were selected randomly. As results, we obtained the optimum condition (1 mg/mL, 20 psi, 10,000 dalton) for removing $PLA_2$ at constant concentration of melittin and apamin and confirmed the separation results by HPLC and SDS-PAGE.
Keywords
honey; bee venom; phospholipase $A_2$; ultrafiltration;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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