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http://dx.doi.org/10.5806/AST.2013.26.6.401

Analysis of respiration gas of a fertile chicken egg during incubation by gas mass spectrometer  

Kim, Hyunjoo (School of Liberal Arts and Sciences, Korea National University of Transportation)
Min, Deullae (Korea Research Institute of Standards and Science)
Kim, Dalho (Korea Research Institute of Standards and Science)
Kim, Jin Seog (Korea Research Institute of Standards and Science)
Publication Information
Analytical Science and Technology / v.26, no.6, 2013 , pp. 401-406 More about this Journal
Abstract
Oxygen($O_2$) consumption and carbon dioxide($CO_2$) excretion of a fertile chicken egg during incubation were measured by a gas mass spectrometer. A closed sample chamber was developed to collect gas samples during the 20 days of artificial incubation of both a fertile and an infertile egg. After leaving an egg in the sample chamber for an hour, using a gas-tight syringe, samples of 2 mL of gas were collected from the closed sample chamber and analyzed using a gas mass spectrometer in 2~4 day intervals. The $O_2$ consumption and $CO_2$ excretion of chicken embryos increased rapidly after 10 days from the starting point of incubation. After 20 days, 23 mL of $O_2$ was consumed and 16 mL of $CO_2$ was excreted per hour. Throughout the whole period of incubation, concentration of $O_2$ decreased 4.3 mol% and $CO_2$ increased only 3.1 mole%, i.e., the mole of consumed $O_2$ and the mole of excreted $CO_2$ were not the same. On the other hand, during the same period, concentration of $N_2$ increased about 1.3 mol% and the increased mole fraction of $N_2$ was comparable with the difference (1.2 mol%) between the mole fraction of consumed $O_2$ and excreted $CO_2$. Therefore, we can attribute the increase of $N_2$ mole% to the difference of mole fraction between consumed $O_2$ and excreted $CO_2$. In this study, through the analysis of gas, we could explain the respiration of a fertile chicken egg during incubation.
Keywords
artificial incubation; gas mass spectrometer; standard gas; oxygen; carbon dioxide; respiration;
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Times Cited By KSCI : 1  (Citation Analysis)
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