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http://dx.doi.org/10.5806/AST.2013.26.6.357

Separation and identification of selenoproteins in selenium-enriched yeast  

Kim, Kyong-Mi (Department of Chemistry Education, Korea National University of Education)
Pak, Yong-Nam (Department of Chemistry Education, Korea National University of Education)
Publication Information
Analytical Science and Technology / v.26, no.6, 2013 , pp. 357-363 More about this Journal
Abstract
Selenium-containing proteins were separated from selenium-enriched yeast (SEY) using Trizol$^{(R)}$ reagent followed by anion exchange (AE) chromatography. This method is simpler and less time consuming than electrophoresis. Five selenium containing proteins were identified by on-line AE HPLC-ICP/MS (high performance liquid chromatography-inductively coupled plasma/mass spectrometry). Each protein was enzymatically hydrolyzed to seleno-amino acids and separated with RP (reverse phase) HPLC for the identification of selenoproteins.
Keywords
Se-enriched yeast; HPLC-ICP/MS; selenoprotein; Trizol$^{(R)}$ reagent; anion exchange chromatography;
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