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http://dx.doi.org/10.5806/AST.2013.26.1.019

Analysis of gibberellic acid from fruits using HPLC/UV-vis  

Ma, Kyung Na (Department of Chemistry, Kyonggi University)
Cho, Hyun-Woo (Department of Chemistry, Kyonggi University)
Myung, Seung-Woon (Department of Chemistry, Kyonggi University)
Publication Information
Analytical Science and Technology / v.26, no.1, 2013 , pp. 19-26 More about this Journal
Abstract
Gibberllic acid ($GA_3$) is one of gibberellins (GAs) that are a class of plant growth hormones that exert profound and diverse effects on plant growth and development. $GA_3$ is essentially non-UV absorbing and is difficult to assay by UV-detector. For effective extraction of gibberellic acid from fruits by using liquid-liquid extraction, optimized pH and extraction solvent were established. The selective and sensitive derivative of $GA_3$ for HPLC/UV-vis was derivatized using phenacyl bromide, and the experimental factors, including reaction time, reaction temperature and amount of derivatizing reagent and base were investigated for the effective synthesis. The derivatized $GA_3$ with phenacyl bromide was effectively analyzed by HPLC/UV-vis. The structure of derivatized $GA_3$ was confirmed by HPLC/ESI-MS. For apple, LOD and LOQ were 0.008 mg/kg and 0.027 mg/kg, respectively. For pear, LOD and LOQ were 0.003 mg/kg, 0.012 mg/kg, respectively. The established method can be applied to more effective analysis of $GA_3$ from plant and food.
Keywords
gibberellic acid; plant hormone; HPLC/UV-vis; derivatization;
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