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http://dx.doi.org/10.5806/AST.2012.25.4.257

Residue analysis of penicillines in livestock and marine products  

Song, Ji-Young (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration)
Hu, Soo-Jung (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration)
Joo, Hyun-Jin (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration)
Kim, Mi-Ok (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration)
Hwang, Joung-Boon (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration)
Han, Yoon-Jung (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration)
Kwon, Yu-Jihn (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration)
Kang, Shin-Jung (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration)
Cho, Dae-Hyun (Hazardous Substances Analysis Division, Gyeoin Regional Food and Drug Administration)
Publication Information
Analytical Science and Technology / v.25, no.4, 2012 , pp. 257-264 More about this Journal
Abstract
Penicillins belong to the ${\beta}$-lactam class of antibiotics, and are frequently used in human and veterinary medicine. Despite the positive effects of these drugs, improper use of penicillins poses a potential health risk to consumers. This study has been undertaken to determinate multi-residues of penicillins, including amoxicillin, ampicillin, oxacillin, bezylpenicillin, cloxacillin, dicloxacillin, and nafcillin, using liquid chromatographic tandem mass spectrometer (LC-MS/MS). The developed method was validated for specificity, precision, recovery, and linearity in livestock and marine products. The analytes were extracted with 80% acetonitrile and clean-up by a single reversed-phase solid-phase extraction step. Six penicillins presented recoveries higher than 76% with the exception of Amoxicillin. Relative standard deviations (RSDs) were not more than 10%. The method was applied to 225 real samples. Benzylpenicillin was detected in 12 livestock products and 7 marine products. Amoxicillin, ampicillin, cloxacilllin, dicloxacillin, nafcillin and oxacillin were not detected. The detected levels were 0.001~0.009 mg/kg in livestock products excluding eggs and milk. In marine products, the detected levels were under 0.03 mg/kg. They were under the MRL levels. As monitoring results, it is identified to be safe but it is considered that safety management of antibiotics should continue by monitoring.
Keywords
livestock product; marine product; LC-MS/MS; monitoring; penicillins;
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