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http://dx.doi.org/10.5806/AST.2011.24.5.345

Determination of finasteride in human serum by LC-MS/MS  

Nam, Hye-Seon (Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration Osong Health Technology Administration Complex)
Nam, Kyong-Hee (Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration Osong Health Technology Administration Complex)
Jung, Su-Hee (Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration Osong Health Technology Administration Complex)
Lee, Jang-Woo (Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration Osong Health Technology Administration Complex)
Kang, Jin-Yeong (Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration Osong Health Technology Administration Complex)
Hong, Soon-Keun (Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration Osong Health Technology Administration Complex)
Kim, Tae-Sung (Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration Osong Health Technology Administration Complex)
Kang, Tae-Seok (Toxicology Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration Osong Health Technology Administration Complex)
Yoon, Hae-Jung (Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration Osong Health Technology Administration Complex)
Lee, Kwang-Ho (Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration Osong Health Technology Administration Complex)
Rhee, Gyu-Seek (Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration Osong Health Technology Administration Complex)
Publication Information
Analytical Science and Technology / v.24, no.5, 2011 , pp. 345-351 More about this Journal
Abstract
A liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS) method was developed and validated for the determination of finasteride in human serum. Beclomethasone was used as internal standard (IS) and liquid-liquid extraction (LLE) using methyl tert-butyl ether (MTBE) was carried out to isolate analyte. The mass transitions monitored in multiple reaction monitoring (MRM) in positive ion mode were m/z 373.2${\rightarrow}$305.2 for finasteride and m/z 409.3${\rightarrow}$391.2 for IS. Retention times of finasteride and IS were 5.81 and 5.46 min, respectively. The limit of quantitation (LOQ) was 0.1 ng/mL and the calibration curve showed good linearity in the range of 0.1~20.0 ng/mL ($R^2$=0.9997). The intra-day assay precision and accuracy were in the range 6.3~10.6% and 97.3~103.6%, respectively, and the inter-day assay precision and accuracy were in the range 0.8~5.2% and 99.8~102.5%, respectively. The sample extract recovery of the method was 80~83%.
Keywords
Finasteride; LC-MS/MS; human serum;
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