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http://dx.doi.org/10.5806/AST.2011.24.1.001

Uncertainty evaluation of the analysis of 11-nor-9-carboxy-Δ9-tetrahydrocannabinol in hair by GC-NCI-MS/MS  

Kim, Jin-Young (Drug Analysis Laboratory, Supreme Prosecutors' Office)
Lee, Jae-Il (Drug Analysis Laboratory, Supreme Prosecutors' Office)
Cheong, Jae-Chul (Drug Analysis Laboratory, Supreme Prosecutors' Office)
Suh, Yong-Jun (Drug Analysis Laboratory, Supreme Prosecutors' Office)
In, Moon-Kyo (Drug Analysis Laboratory, Supreme Prosecutors' Office)
Publication Information
Analytical Science and Technology / v.24, no.1, 2011 , pp. 1-9 More about this Journal
Abstract
We described an estimation of measurement uncertainty in quantitative analysis of 11-nor-9-carboxy-${\Delta}^9$-tetrahydrocannabinol (THC-COOH), the metabolite of ${\Delta}^9$-tetrahydrocannabinol, in hair samples by using the bead-assisted liquid-liquid extraction and gas chromatography-tandem mass spectrometric (GC-NCI-MS/MS) detection. Traceability of measurement was established through the use of reference materials, calibrated volumetric tubes, volume measuring devices, and measuring instruments. The analytical results were compared and the different contributions to the uncertainty were evaluated. Inter-day variation was performed by using statistical analysis of several indicative factors. Measurement uncertainty associated with the analyte in real forensic hair samples were estimated using QC data. The major factor of contribution to combined standard uncertainty was inter-day repeatability, while those associated with preparation of analytical standard and also sample of weight were insignificant considering the degree of contribution. Relative uncertainty of relative extended standard uncertainty divided into the measured concentration of the analyte was 17% in a hair sample. The uncertainty of result evaluation will be invaluable to improve quality of the analysis.
Keywords
Measurement uncertainty; hair analysis; 11-nor-9-carboxy-${\Delta}^9$-tetrahydrocannabinol; GC-NCI-MS/MS;
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  • Reference
1 K. M. Clauwaert, J. F. Van Bocxlaer, W. E. Lambert and A. P. De Leenheer, Forensic Sci. Int., 110, 157-166(2000).   DOI   ScienceOn
2 H. Sachs and P. Kintz, J. Chromatogr. B, 713, 147-161(1998).   DOI   ScienceOn
3 M. Rothe, F. Pragst, K. Spiegel, T. Harrach, K. Fischer and J. Kunkel, Forensic Sci. Int., 89, 111-128(1997).   DOI   ScienceOn
4 J. Y. Kim and M. K. In, Rapid Commun. Mass Spectrom., 21, 1339-1342(2007).   DOI   ScienceOn
5 한국표준과학연구원, "측정불확도 표현지침", 1998.
6 Korea Laboratory Accreditation Scheme (KOLAS), "측정결과의 불확도 산정 및 표현을 위한 지침", 2000.
7 KS Q 4001, "측정결과의 불확도 추정 및 표현을 위한 지침", 2005.
8 ISO, "Guide to the Expression of Uncertainty in Measurement", ISO, Geneva, Switzerland, 1993.
9 EURACHEM/CITAC, "Quantifying Uncertainty in Analytical Measurement", 2nd Ed., EURACHEM/CITAC Guide CG 4, 2000.
10 한국표준과학연구원, "도전! 측정불확도, 기초에서 중급까지", 2008.
11 대검찰청, "2009 마약류범죄백서", 2010.
12 M. E. Wall, D. R. Brine, G. A. Brine, C. G. Pitt, R. I. Freudenthal and H. D. Christensen, J. Am. Chem. Soc., 92, 3466-3468(1970).   DOI
13 S. Agurell, I. M. Nilsson and M. Widman, In Marihuana Chemistry, 'Biochemistry and Cellular Effects', 141, Springer-Verlag, New York, U.S.A., 1976.
14 U.S. Department of Heath and Human Servies, 'NIDA Research Monograph 42', 43, National Institute on Drug Abuse, Rockville, MD, U.S.A., 1982.
15 A. P. Mason and A. J. McBay, J. Forensic Sci., 30, 615-631(1985).
16 T. Saito, I. Yamamoto, T. Kusakabe, X. L. Huang, N. Yukawa and S. Takeichi, Forensic Sci. Int., 112, 65-71(2000).   DOI   ScienceOn