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http://dx.doi.org/10.5806/AST.2010.23.2.172

Study on the immobilization of plant glutathione S-transferase for development of herbicide detection kit  

Cho, Hyun-Young (Department of Chemistry, College of Natural Sciences, Chung-Ang University)
Lee, Jin-Joo (Department of Chemistry, College of Natural Sciences, Chung-Ang University)
Kong, Kwang-Hoon (Department of Chemistry, College of Natural Sciences, Chung-Ang University)
Publication Information
Analytical Science and Technology / v.23, no.2, 2010 , pp. 172-178 More about this Journal
Abstract
Glutathione S-transferase is known to play a crucial role in detoxification in many cases. To develop a herbicide detection biosensor, we in this study attempted to immobilize glutathione S-transferase enzyme on solid supports, polystyrene and agarose, and Na-alginate. These matrixes were attractive materials for the construction of biosensors and might also have utility for the production of immobilized enzyme bioreactors. We also compared the activities of glutathione-S-transferase immobilized OsGSTF3 and free OsGSTF3. The specific activity of the free enzyme in solution was 3.3 higher than the immobilized enzyme. These results suggest that 50% of the enzyme was bound with the catalytic site in polystyrene-alkylamine bead and immobilized enzymes showed 80% remaining activity until 3 times reuse.
Keywords
biosensor; glutathione S-transferase; herbicide detoxification; protein immobilization;
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